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Transformation of Stilbene Glucosides From Reynoutria multiflora During Processing
The root of Reynoutria multiflora Thunb. Moldenke (RM, syn.: Polygonum multiflorum Thunb.) has been widely used in TCM clinical practice for centuries. The raw R. multiflora (RRM) should be processed before use, in order to reduce toxicity and increase efficiency. However, the content of trans-2, 3,...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082504/ https://www.ncbi.nlm.nih.gov/pubmed/35548344 http://dx.doi.org/10.3389/fphar.2022.757490 |
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author | Bai, Junqi Chen, Wanting Huang, Juan Su, He Zhang, Danchun Xu, Wen Zhang, Jing Huang, Zhihai Qiu, Xiaohui |
author_facet | Bai, Junqi Chen, Wanting Huang, Juan Su, He Zhang, Danchun Xu, Wen Zhang, Jing Huang, Zhihai Qiu, Xiaohui |
author_sort | Bai, Junqi |
collection | PubMed |
description | The root of Reynoutria multiflora Thunb. Moldenke (RM, syn.: Polygonum multiflorum Thunb.) has been widely used in TCM clinical practice for centuries. The raw R. multiflora (RRM) should be processed before use, in order to reduce toxicity and increase efficiency. However, the content of trans-2, 3, 5, 4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside (trans-THSG), which is considered to be the main medicinal ingredient, decreases in this process. In order to understand the changes of stilbene glycosides raw R. multiflora (RRM) and processed R. multiflora (PRM), a simple and effective method was developed by ultra high performance liquid chromatography tandem quadrupole/electrostatic field orbitrap high-resolution mass spectrometry (UHPLC-Q-Exactive plus orbitrap MS/MS). The content and quantity of stilbene glycosideshave undergone tremendous changes during the process. Seven parent nucleus of stilbene glycosides and 55 substituents, including 5-HMF and a series of derivatives, were identified in PM. 146 stilbene glycosides were detected in RRM, The number of detected compounds increased from 198 to 219 as the processing time increased from 4 to 32 h. Among the detected compounds, 102 stilbene glycosides may be potential new compounds. And the changing trend of the compounds can be summarized in 3 forms: gradually increased, gradually decreased, first increased and then decreased or decreased first. The content of trans-THSG was indeed decreased during processing, as it was converted into a series of derivatives through the esterification reaction with small molecular compounds. The clarification of secondary metabolite group can provide a basis for the follow-up study on the mechanism of pharmacodynamics and toxicity of PM, and for screening of relevant quality markers. |
format | Online Article Text |
id | pubmed-9082504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90825042022-05-10 Transformation of Stilbene Glucosides From Reynoutria multiflora During Processing Bai, Junqi Chen, Wanting Huang, Juan Su, He Zhang, Danchun Xu, Wen Zhang, Jing Huang, Zhihai Qiu, Xiaohui Front Pharmacol Pharmacology The root of Reynoutria multiflora Thunb. Moldenke (RM, syn.: Polygonum multiflorum Thunb.) has been widely used in TCM clinical practice for centuries. The raw R. multiflora (RRM) should be processed before use, in order to reduce toxicity and increase efficiency. However, the content of trans-2, 3, 5, 4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside (trans-THSG), which is considered to be the main medicinal ingredient, decreases in this process. In order to understand the changes of stilbene glycosides raw R. multiflora (RRM) and processed R. multiflora (PRM), a simple and effective method was developed by ultra high performance liquid chromatography tandem quadrupole/electrostatic field orbitrap high-resolution mass spectrometry (UHPLC-Q-Exactive plus orbitrap MS/MS). The content and quantity of stilbene glycosideshave undergone tremendous changes during the process. Seven parent nucleus of stilbene glycosides and 55 substituents, including 5-HMF and a series of derivatives, were identified in PM. 146 stilbene glycosides were detected in RRM, The number of detected compounds increased from 198 to 219 as the processing time increased from 4 to 32 h. Among the detected compounds, 102 stilbene glycosides may be potential new compounds. And the changing trend of the compounds can be summarized in 3 forms: gradually increased, gradually decreased, first increased and then decreased or decreased first. The content of trans-THSG was indeed decreased during processing, as it was converted into a series of derivatives through the esterification reaction with small molecular compounds. The clarification of secondary metabolite group can provide a basis for the follow-up study on the mechanism of pharmacodynamics and toxicity of PM, and for screening of relevant quality markers. Frontiers Media S.A. 2022-04-25 /pmc/articles/PMC9082504/ /pubmed/35548344 http://dx.doi.org/10.3389/fphar.2022.757490 Text en Copyright © 2022 Bai, Chen, Huang, Su, Zhang, Xu, Zhang, Huang and Qiu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Bai, Junqi Chen, Wanting Huang, Juan Su, He Zhang, Danchun Xu, Wen Zhang, Jing Huang, Zhihai Qiu, Xiaohui Transformation of Stilbene Glucosides From Reynoutria multiflora During Processing |
title | Transformation of Stilbene Glucosides From Reynoutria multiflora During Processing |
title_full | Transformation of Stilbene Glucosides From Reynoutria multiflora During Processing |
title_fullStr | Transformation of Stilbene Glucosides From Reynoutria multiflora During Processing |
title_full_unstemmed | Transformation of Stilbene Glucosides From Reynoutria multiflora During Processing |
title_short | Transformation of Stilbene Glucosides From Reynoutria multiflora During Processing |
title_sort | transformation of stilbene glucosides from reynoutria multiflora during processing |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082504/ https://www.ncbi.nlm.nih.gov/pubmed/35548344 http://dx.doi.org/10.3389/fphar.2022.757490 |
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