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Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process
Reconstitution of biologically active polymersomes from the frozen or solid state into any fluid state is still a challenging issue for the design of new biological experiments and for the formulation of therapeutic agents. To gain knowledge about the reconstitution of pH-responsive and photo-crossl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082561/ https://www.ncbi.nlm.nih.gov/pubmed/35539787 http://dx.doi.org/10.1039/c8ra03964j |
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author | Ccorahua, Robert Moreno, Silvia Gumz, Hannes Sahre, Karin Voit, Brigitte Appelhans, Dietmar |
author_facet | Ccorahua, Robert Moreno, Silvia Gumz, Hannes Sahre, Karin Voit, Brigitte Appelhans, Dietmar |
author_sort | Ccorahua, Robert |
collection | PubMed |
description | Reconstitution of biologically active polymersomes from the frozen or solid state into any fluid state is still a challenging issue for the design of new biological experiments and for the formulation of therapeutic agents. To gain knowledge about the reconstitution of pH-responsive and photo-crosslinked polymersomes, surface-functionalized and enzyme-containing polymersomers were cryogenically frozen (−20 °C) or freeze-dried with inulin as the lyoprotectant (0.1% w/v) and stored for a defined time period. Reconstituting those polymersomes in solution by thawing or a re-dispersing process revealed their original physical properties as well as their function as a pH-switchable enzymatic nanoreactor. |
format | Online Article Text |
id | pubmed-9082561 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90825612022-05-09 Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process Ccorahua, Robert Moreno, Silvia Gumz, Hannes Sahre, Karin Voit, Brigitte Appelhans, Dietmar RSC Adv Chemistry Reconstitution of biologically active polymersomes from the frozen or solid state into any fluid state is still a challenging issue for the design of new biological experiments and for the formulation of therapeutic agents. To gain knowledge about the reconstitution of pH-responsive and photo-crosslinked polymersomes, surface-functionalized and enzyme-containing polymersomers were cryogenically frozen (−20 °C) or freeze-dried with inulin as the lyoprotectant (0.1% w/v) and stored for a defined time period. Reconstituting those polymersomes in solution by thawing or a re-dispersing process revealed their original physical properties as well as their function as a pH-switchable enzymatic nanoreactor. The Royal Society of Chemistry 2018-07-17 /pmc/articles/PMC9082561/ /pubmed/35539787 http://dx.doi.org/10.1039/c8ra03964j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Ccorahua, Robert Moreno, Silvia Gumz, Hannes Sahre, Karin Voit, Brigitte Appelhans, Dietmar Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process |
title | Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process |
title_full | Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process |
title_fullStr | Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process |
title_full_unstemmed | Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process |
title_short | Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process |
title_sort | reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082561/ https://www.ncbi.nlm.nih.gov/pubmed/35539787 http://dx.doi.org/10.1039/c8ra03964j |
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