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Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process

Reconstitution of biologically active polymersomes from the frozen or solid state into any fluid state is still a challenging issue for the design of new biological experiments and for the formulation of therapeutic agents. To gain knowledge about the reconstitution of pH-responsive and photo-crossl...

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Autores principales: Ccorahua, Robert, Moreno, Silvia, Gumz, Hannes, Sahre, Karin, Voit, Brigitte, Appelhans, Dietmar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082561/
https://www.ncbi.nlm.nih.gov/pubmed/35539787
http://dx.doi.org/10.1039/c8ra03964j
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author Ccorahua, Robert
Moreno, Silvia
Gumz, Hannes
Sahre, Karin
Voit, Brigitte
Appelhans, Dietmar
author_facet Ccorahua, Robert
Moreno, Silvia
Gumz, Hannes
Sahre, Karin
Voit, Brigitte
Appelhans, Dietmar
author_sort Ccorahua, Robert
collection PubMed
description Reconstitution of biologically active polymersomes from the frozen or solid state into any fluid state is still a challenging issue for the design of new biological experiments and for the formulation of therapeutic agents. To gain knowledge about the reconstitution of pH-responsive and photo-crosslinked polymersomes, surface-functionalized and enzyme-containing polymersomers were cryogenically frozen (−20 °C) or freeze-dried with inulin as the lyoprotectant (0.1% w/v) and stored for a defined time period. Reconstituting those polymersomes in solution by thawing or a re-dispersing process revealed their original physical properties as well as their function as a pH-switchable enzymatic nanoreactor.
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spelling pubmed-90825612022-05-09 Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process Ccorahua, Robert Moreno, Silvia Gumz, Hannes Sahre, Karin Voit, Brigitte Appelhans, Dietmar RSC Adv Chemistry Reconstitution of biologically active polymersomes from the frozen or solid state into any fluid state is still a challenging issue for the design of new biological experiments and for the formulation of therapeutic agents. To gain knowledge about the reconstitution of pH-responsive and photo-crosslinked polymersomes, surface-functionalized and enzyme-containing polymersomers were cryogenically frozen (−20 °C) or freeze-dried with inulin as the lyoprotectant (0.1% w/v) and stored for a defined time period. Reconstituting those polymersomes in solution by thawing or a re-dispersing process revealed their original physical properties as well as their function as a pH-switchable enzymatic nanoreactor. The Royal Society of Chemistry 2018-07-17 /pmc/articles/PMC9082561/ /pubmed/35539787 http://dx.doi.org/10.1039/c8ra03964j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Ccorahua, Robert
Moreno, Silvia
Gumz, Hannes
Sahre, Karin
Voit, Brigitte
Appelhans, Dietmar
Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process
title Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process
title_full Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process
title_fullStr Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process
title_full_unstemmed Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process
title_short Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process
title_sort reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082561/
https://www.ncbi.nlm.nih.gov/pubmed/35539787
http://dx.doi.org/10.1039/c8ra03964j
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