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Reduced graphene oxide-polyaniline film as enhanced sensing interface for the detection of loop-mediated-isothermal-amplification products by open circuit potential measurement
The development of low cost, portable diagnostic tools for in-field detection of viruses and other pathogenic microorganisms is in great demand but remains challenging. In this study, a novel approach based on reduced graphene oxide-polyaniline (rGO-PANi) film for the in situ detection of loop-media...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082585/ https://www.ncbi.nlm.nih.gov/pubmed/35539802 http://dx.doi.org/10.1039/c8ra04050h |
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author | Thu, Vu Thi Tien, Bui Quang Ngoc Nga, Dau Thi Thanh, Ly Cong Sinh, Le Hoang Le, Tu Cam Lam, Tran Dai |
author_facet | Thu, Vu Thi Tien, Bui Quang Ngoc Nga, Dau Thi Thanh, Ly Cong Sinh, Le Hoang Le, Tu Cam Lam, Tran Dai |
author_sort | Thu, Vu Thi |
collection | PubMed |
description | The development of low cost, portable diagnostic tools for in-field detection of viruses and other pathogenic microorganisms is in great demand but remains challenging. In this study, a novel approach based on reduced graphene oxide-polyaniline (rGO-PANi) film for the in situ detection of loop-mediated-isothermal-amplification (LAMP) products by means of open circuit potential measurement is proposed. The pH-sensitive conducting polymer PANi was electro-deposited onto rGO coated screen printed electrodes and tuned to be at the emeraldine state at which the pH sensitivity was maximized. By combining PANi and rGO, the pH sensitivity of the system was modulated up to about −64 mV per pH unit. This enabled the number of amplified amplicons resulting from the isothermal amplification process to be monitored. The sensor was then examined for monitoring LAMP reactions using Hepatitis B virus (HBV) as a model. This simple, low-cost, reproducible and sensitive interfacing layer is expected to provide a new possibility for designing point-of-care sensors under limited-resource conditions. |
format | Online Article Text |
id | pubmed-9082585 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90825852022-05-09 Reduced graphene oxide-polyaniline film as enhanced sensing interface for the detection of loop-mediated-isothermal-amplification products by open circuit potential measurement Thu, Vu Thi Tien, Bui Quang Ngoc Nga, Dau Thi Thanh, Ly Cong Sinh, Le Hoang Le, Tu Cam Lam, Tran Dai RSC Adv Chemistry The development of low cost, portable diagnostic tools for in-field detection of viruses and other pathogenic microorganisms is in great demand but remains challenging. In this study, a novel approach based on reduced graphene oxide-polyaniline (rGO-PANi) film for the in situ detection of loop-mediated-isothermal-amplification (LAMP) products by means of open circuit potential measurement is proposed. The pH-sensitive conducting polymer PANi was electro-deposited onto rGO coated screen printed electrodes and tuned to be at the emeraldine state at which the pH sensitivity was maximized. By combining PANi and rGO, the pH sensitivity of the system was modulated up to about −64 mV per pH unit. This enabled the number of amplified amplicons resulting from the isothermal amplification process to be monitored. The sensor was then examined for monitoring LAMP reactions using Hepatitis B virus (HBV) as a model. This simple, low-cost, reproducible and sensitive interfacing layer is expected to provide a new possibility for designing point-of-care sensors under limited-resource conditions. The Royal Society of Chemistry 2018-07-16 /pmc/articles/PMC9082585/ /pubmed/35539802 http://dx.doi.org/10.1039/c8ra04050h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Thu, Vu Thi Tien, Bui Quang Ngoc Nga, Dau Thi Thanh, Ly Cong Sinh, Le Hoang Le, Tu Cam Lam, Tran Dai Reduced graphene oxide-polyaniline film as enhanced sensing interface for the detection of loop-mediated-isothermal-amplification products by open circuit potential measurement |
title | Reduced graphene oxide-polyaniline film as enhanced sensing interface for the detection of loop-mediated-isothermal-amplification products by open circuit potential measurement |
title_full | Reduced graphene oxide-polyaniline film as enhanced sensing interface for the detection of loop-mediated-isothermal-amplification products by open circuit potential measurement |
title_fullStr | Reduced graphene oxide-polyaniline film as enhanced sensing interface for the detection of loop-mediated-isothermal-amplification products by open circuit potential measurement |
title_full_unstemmed | Reduced graphene oxide-polyaniline film as enhanced sensing interface for the detection of loop-mediated-isothermal-amplification products by open circuit potential measurement |
title_short | Reduced graphene oxide-polyaniline film as enhanced sensing interface for the detection of loop-mediated-isothermal-amplification products by open circuit potential measurement |
title_sort | reduced graphene oxide-polyaniline film as enhanced sensing interface for the detection of loop-mediated-isothermal-amplification products by open circuit potential measurement |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082585/ https://www.ncbi.nlm.nih.gov/pubmed/35539802 http://dx.doi.org/10.1039/c8ra04050h |
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