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Formation of functional nanobiocatalysts with a novel and encouraging immobilization approach and their versatile bioanalytical applications

The discovery of functional organic–inorganic hybrid nanoflowers (FNFs) consisting of proteins/enzymes as the organic components and Cu(ii) ion as the inorganic component has made an enormous impact on enzyme immobilization studies. The FNFs synthesized by an encouraging and novel approach not only...

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Autores principales: Celik, Cagla, Tasdemir, Didar, Demirbas, Ayse, Katı, Ahmet, Gul, O. Tolga, Cimen, Behzat, Ocsoy, Ismail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082721/
https://www.ncbi.nlm.nih.gov/pubmed/35539799
http://dx.doi.org/10.1039/c8ra03250e
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author Celik, Cagla
Tasdemir, Didar
Demirbas, Ayse
Katı, Ahmet
Gul, O. Tolga
Cimen, Behzat
Ocsoy, Ismail
author_facet Celik, Cagla
Tasdemir, Didar
Demirbas, Ayse
Katı, Ahmet
Gul, O. Tolga
Cimen, Behzat
Ocsoy, Ismail
author_sort Celik, Cagla
collection PubMed
description The discovery of functional organic–inorganic hybrid nanoflowers (FNFs) consisting of proteins/enzymes as the organic components and Cu(ii) ion as the inorganic component has made an enormous impact on enzyme immobilization studies. The FNFs synthesized by an encouraging and novel approach not only showed high stabilities but also much enhanced catalytic activities as compared to free and conventionally immobilized enzymes. A recent development demonstrated that FNF formation has moved beyond the initial discovery in which enzymes and Cu(2+) ions used as the organic and inorganic parts, respectively, are replaced with new organic (chitosan, amino acid and plant extracts) and inorganic (Cu(2+) and Fe(2+)) materials. The new organic materials incorporated into FNFs act as Fenton-like agents and then show peroxidase-like activity owing to the metal ions and the porous structure of FNFs in the presence of hydrogen peroxide (H(2)O(2)). All FNFs have been widely utilized in many different scientific and industrial fields due to their greatly enhanced activities and stabilities. This review focuses primarily on the preparation, characterization, and bioanalytical applications of FNFs and explains the mechanisms of their formation and enhanced activities and stabilities.
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spelling pubmed-90827212022-05-09 Formation of functional nanobiocatalysts with a novel and encouraging immobilization approach and their versatile bioanalytical applications Celik, Cagla Tasdemir, Didar Demirbas, Ayse Katı, Ahmet Gul, O. Tolga Cimen, Behzat Ocsoy, Ismail RSC Adv Chemistry The discovery of functional organic–inorganic hybrid nanoflowers (FNFs) consisting of proteins/enzymes as the organic components and Cu(ii) ion as the inorganic component has made an enormous impact on enzyme immobilization studies. The FNFs synthesized by an encouraging and novel approach not only showed high stabilities but also much enhanced catalytic activities as compared to free and conventionally immobilized enzymes. A recent development demonstrated that FNF formation has moved beyond the initial discovery in which enzymes and Cu(2+) ions used as the organic and inorganic parts, respectively, are replaced with new organic (chitosan, amino acid and plant extracts) and inorganic (Cu(2+) and Fe(2+)) materials. The new organic materials incorporated into FNFs act as Fenton-like agents and then show peroxidase-like activity owing to the metal ions and the porous structure of FNFs in the presence of hydrogen peroxide (H(2)O(2)). All FNFs have been widely utilized in many different scientific and industrial fields due to their greatly enhanced activities and stabilities. This review focuses primarily on the preparation, characterization, and bioanalytical applications of FNFs and explains the mechanisms of their formation and enhanced activities and stabilities. The Royal Society of Chemistry 2018-07-16 /pmc/articles/PMC9082721/ /pubmed/35539799 http://dx.doi.org/10.1039/c8ra03250e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Celik, Cagla
Tasdemir, Didar
Demirbas, Ayse
Katı, Ahmet
Gul, O. Tolga
Cimen, Behzat
Ocsoy, Ismail
Formation of functional nanobiocatalysts with a novel and encouraging immobilization approach and their versatile bioanalytical applications
title Formation of functional nanobiocatalysts with a novel and encouraging immobilization approach and their versatile bioanalytical applications
title_full Formation of functional nanobiocatalysts with a novel and encouraging immobilization approach and their versatile bioanalytical applications
title_fullStr Formation of functional nanobiocatalysts with a novel and encouraging immobilization approach and their versatile bioanalytical applications
title_full_unstemmed Formation of functional nanobiocatalysts with a novel and encouraging immobilization approach and their versatile bioanalytical applications
title_short Formation of functional nanobiocatalysts with a novel and encouraging immobilization approach and their versatile bioanalytical applications
title_sort formation of functional nanobiocatalysts with a novel and encouraging immobilization approach and their versatile bioanalytical applications
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082721/
https://www.ncbi.nlm.nih.gov/pubmed/35539799
http://dx.doi.org/10.1039/c8ra03250e
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