Ca(V)β-subunit dependence of forward and reverse trafficking of Ca(V)1.2 calcium channels

Auxiliary Ca(V)β subunits interact with the pore forming Ca(V)α(1) subunit to promote the plasma membrane expression of high voltage-activated calcium channels and to modulate the biophysical properties of Ca(2+) currents. However, the effect of Ca(V)β subunits on channel trafficking to and from the...

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Autores principales: Ferron, Laurent, Guderyan, Sydney D., Smith, Ethan J., Zamponi, Gerald W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082888/
https://www.ncbi.nlm.nih.gov/pubmed/35534894
http://dx.doi.org/10.1186/s13041-022-00930-x
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author Ferron, Laurent
Guderyan, Sydney D.
Smith, Ethan J.
Zamponi, Gerald W.
author_facet Ferron, Laurent
Guderyan, Sydney D.
Smith, Ethan J.
Zamponi, Gerald W.
author_sort Ferron, Laurent
collection PubMed
description Auxiliary Ca(V)β subunits interact with the pore forming Ca(V)α(1) subunit to promote the plasma membrane expression of high voltage-activated calcium channels and to modulate the biophysical properties of Ca(2+) currents. However, the effect of Ca(V)β subunits on channel trafficking to and from the plasma membrane is still controversial. Here, we have investigated the impact of Ca(V)β1b and Ca(V)β2a subunits on plasma membrane trafficking of Ca(V)1.2 using a live-labeling strategy. We show that the Ca(V)β1b subunit is more potent in increasing Ca(V)1.2 expression at the plasma membrane than the Ca(V)β2a subunit and that this effect is not related to modification of intracellular trafficking of the channel (i.e. neither forward trafficking, nor recycling, nor endocytosis). We conclude that the differential effect of Ca(V)β subunit subtypes on Ca(V)1.2 surface expression is likely due to their differential ability to protect Ca(V)1.2 from degradation.
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spelling pubmed-90828882022-05-10 Ca(V)β-subunit dependence of forward and reverse trafficking of Ca(V)1.2 calcium channels Ferron, Laurent Guderyan, Sydney D. Smith, Ethan J. Zamponi, Gerald W. Mol Brain Short Report Auxiliary Ca(V)β subunits interact with the pore forming Ca(V)α(1) subunit to promote the plasma membrane expression of high voltage-activated calcium channels and to modulate the biophysical properties of Ca(2+) currents. However, the effect of Ca(V)β subunits on channel trafficking to and from the plasma membrane is still controversial. Here, we have investigated the impact of Ca(V)β1b and Ca(V)β2a subunits on plasma membrane trafficking of Ca(V)1.2 using a live-labeling strategy. We show that the Ca(V)β1b subunit is more potent in increasing Ca(V)1.2 expression at the plasma membrane than the Ca(V)β2a subunit and that this effect is not related to modification of intracellular trafficking of the channel (i.e. neither forward trafficking, nor recycling, nor endocytosis). We conclude that the differential effect of Ca(V)β subunit subtypes on Ca(V)1.2 surface expression is likely due to their differential ability to protect Ca(V)1.2 from degradation. BioMed Central 2022-05-09 /pmc/articles/PMC9082888/ /pubmed/35534894 http://dx.doi.org/10.1186/s13041-022-00930-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Ferron, Laurent
Guderyan, Sydney D.
Smith, Ethan J.
Zamponi, Gerald W.
Ca(V)β-subunit dependence of forward and reverse trafficking of Ca(V)1.2 calcium channels
title Ca(V)β-subunit dependence of forward and reverse trafficking of Ca(V)1.2 calcium channels
title_full Ca(V)β-subunit dependence of forward and reverse trafficking of Ca(V)1.2 calcium channels
title_fullStr Ca(V)β-subunit dependence of forward and reverse trafficking of Ca(V)1.2 calcium channels
title_full_unstemmed Ca(V)β-subunit dependence of forward and reverse trafficking of Ca(V)1.2 calcium channels
title_short Ca(V)β-subunit dependence of forward and reverse trafficking of Ca(V)1.2 calcium channels
title_sort ca(v)β-subunit dependence of forward and reverse trafficking of ca(v)1.2 calcium channels
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082888/
https://www.ncbi.nlm.nih.gov/pubmed/35534894
http://dx.doi.org/10.1186/s13041-022-00930-x
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