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A Combined Human in Silico and CRISPR/Cas9-Mediated in Vivo Zebrafish Based Approach to Provide Phenotypic Data for Supporting Early Target Validation

The clinical heterogeneity of heart failure has challenged our understanding of the underlying genetic mechanisms of this disease. In this respect, large-scale patient DNA sequencing studies have become an invaluable strategy for identifying potential genetic contributing factors. The complex aetiol...

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Autores principales: Winter, Matthew J., Ono, Yosuke, Ball, Jonathan S., Walentinsson, Anna, Michaelsson, Erik, Tochwin, Anna, Scholpp, Steffen, Tyler, Charles R., Rees, Steve, Hetheridge, Malcolm J, Bohlooly-Y, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082939/
https://www.ncbi.nlm.nih.gov/pubmed/35548346
http://dx.doi.org/10.3389/fphar.2022.827686
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author Winter, Matthew J.
Ono, Yosuke
Ball, Jonathan S.
Walentinsson, Anna
Michaelsson, Erik
Tochwin, Anna
Scholpp, Steffen
Tyler, Charles R.
Rees, Steve
Hetheridge, Malcolm J
Bohlooly-Y, Mohammad
author_facet Winter, Matthew J.
Ono, Yosuke
Ball, Jonathan S.
Walentinsson, Anna
Michaelsson, Erik
Tochwin, Anna
Scholpp, Steffen
Tyler, Charles R.
Rees, Steve
Hetheridge, Malcolm J
Bohlooly-Y, Mohammad
author_sort Winter, Matthew J.
collection PubMed
description The clinical heterogeneity of heart failure has challenged our understanding of the underlying genetic mechanisms of this disease. In this respect, large-scale patient DNA sequencing studies have become an invaluable strategy for identifying potential genetic contributing factors. The complex aetiology of heart failure, however, also means that in vivo models are vital to understand the links between genetic perturbations and functional impacts as part of the process for validating potential new drug targets. Traditional approaches (e.g., genetically-modified mice) are optimal for assessing small numbers of genes, but less practical when multiple genes are identified. The zebrafish, in contrast, offers great potential for higher throughput in vivo gene functional assessment to aid target prioritisation, by providing more confidence in target relevance and facilitating gene selection for definitive loss of function studies undertaken in mice. Here we used whole-exome sequencing and bioinformatics on human patient data to identify 3 genes (API5, HSPB7, and LMO2) suggestively associated with heart failure that were also predicted to play a broader role in disease aetiology. The role of these genes in cardiovascular system development and function was then further investigated using in vivo CRISPR/Cas9-mediated gene mutation analysis in zebrafish. We observed multiple impacts in F0 knockout zebrafish embryos (crispants) following effective somatic mutation, including changes in ventricle size, pericardial oedema, and chamber malformation. In the case of lmo2, there was also a significant impact on cardiovascular function as well as an expected reduction in erythropoiesis. The data generated from both the human in silico and zebrafish in vivo assessments undertaken supports further investigation of the potential roles of API5, HSPB7, and LMO2 in human cardiovascular disease. The data presented also supports the use of human in silico genetic variant analysis, in combination with zebrafish crispant phenotyping, as a powerful approach for assessing gene function as part of an integrated multi-level drug target validation strategy.
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spelling pubmed-90829392022-05-10 A Combined Human in Silico and CRISPR/Cas9-Mediated in Vivo Zebrafish Based Approach to Provide Phenotypic Data for Supporting Early Target Validation Winter, Matthew J. Ono, Yosuke Ball, Jonathan S. Walentinsson, Anna Michaelsson, Erik Tochwin, Anna Scholpp, Steffen Tyler, Charles R. Rees, Steve Hetheridge, Malcolm J Bohlooly-Y, Mohammad Front Pharmacol Pharmacology The clinical heterogeneity of heart failure has challenged our understanding of the underlying genetic mechanisms of this disease. In this respect, large-scale patient DNA sequencing studies have become an invaluable strategy for identifying potential genetic contributing factors. The complex aetiology of heart failure, however, also means that in vivo models are vital to understand the links between genetic perturbations and functional impacts as part of the process for validating potential new drug targets. Traditional approaches (e.g., genetically-modified mice) are optimal for assessing small numbers of genes, but less practical when multiple genes are identified. The zebrafish, in contrast, offers great potential for higher throughput in vivo gene functional assessment to aid target prioritisation, by providing more confidence in target relevance and facilitating gene selection for definitive loss of function studies undertaken in mice. Here we used whole-exome sequencing and bioinformatics on human patient data to identify 3 genes (API5, HSPB7, and LMO2) suggestively associated with heart failure that were also predicted to play a broader role in disease aetiology. The role of these genes in cardiovascular system development and function was then further investigated using in vivo CRISPR/Cas9-mediated gene mutation analysis in zebrafish. We observed multiple impacts in F0 knockout zebrafish embryos (crispants) following effective somatic mutation, including changes in ventricle size, pericardial oedema, and chamber malformation. In the case of lmo2, there was also a significant impact on cardiovascular function as well as an expected reduction in erythropoiesis. The data generated from both the human in silico and zebrafish in vivo assessments undertaken supports further investigation of the potential roles of API5, HSPB7, and LMO2 in human cardiovascular disease. The data presented also supports the use of human in silico genetic variant analysis, in combination with zebrafish crispant phenotyping, as a powerful approach for assessing gene function as part of an integrated multi-level drug target validation strategy. Frontiers Media S.A. 2022-04-25 /pmc/articles/PMC9082939/ /pubmed/35548346 http://dx.doi.org/10.3389/fphar.2022.827686 Text en Copyright © 2022 Winter, Ono, Ball, Walentinsson, Michaelsson, Tochwin, Scholpp, Tyler, Rees, Hetheridge and Bohlooly-Y. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Winter, Matthew J.
Ono, Yosuke
Ball, Jonathan S.
Walentinsson, Anna
Michaelsson, Erik
Tochwin, Anna
Scholpp, Steffen
Tyler, Charles R.
Rees, Steve
Hetheridge, Malcolm J
Bohlooly-Y, Mohammad
A Combined Human in Silico and CRISPR/Cas9-Mediated in Vivo Zebrafish Based Approach to Provide Phenotypic Data for Supporting Early Target Validation
title A Combined Human in Silico and CRISPR/Cas9-Mediated in Vivo Zebrafish Based Approach to Provide Phenotypic Data for Supporting Early Target Validation
title_full A Combined Human in Silico and CRISPR/Cas9-Mediated in Vivo Zebrafish Based Approach to Provide Phenotypic Data for Supporting Early Target Validation
title_fullStr A Combined Human in Silico and CRISPR/Cas9-Mediated in Vivo Zebrafish Based Approach to Provide Phenotypic Data for Supporting Early Target Validation
title_full_unstemmed A Combined Human in Silico and CRISPR/Cas9-Mediated in Vivo Zebrafish Based Approach to Provide Phenotypic Data for Supporting Early Target Validation
title_short A Combined Human in Silico and CRISPR/Cas9-Mediated in Vivo Zebrafish Based Approach to Provide Phenotypic Data for Supporting Early Target Validation
title_sort combined human in silico and crispr/cas9-mediated in vivo zebrafish based approach to provide phenotypic data for supporting early target validation
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082939/
https://www.ncbi.nlm.nih.gov/pubmed/35548346
http://dx.doi.org/10.3389/fphar.2022.827686
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