Cargando…

Decorating a single giant DNA with gold nanoparticles

We decorated a single giant DNA (1.66 × 10(5) base pairs) with gold nanoparticles through the simple procedure of mild warming, without denaturation of the DNA molecule. Single-molecule observation with fluorescence microscopy revealed that individual decorated DNA molecules stay in the bulk solutio...

Descripción completa

Detalles Bibliográficos
Autores principales: Carnerero, Jose M., Masuoka, Shinsuke, Baba, Hikari, Yoshikawa, Yuko, Prado-Gotor, Rafael, Yoshikawa, Kenichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083275/
https://www.ncbi.nlm.nih.gov/pubmed/35541036
http://dx.doi.org/10.1039/c8ra05088k
_version_ 1784703387834515456
author Carnerero, Jose M.
Masuoka, Shinsuke
Baba, Hikari
Yoshikawa, Yuko
Prado-Gotor, Rafael
Yoshikawa, Kenichi
author_facet Carnerero, Jose M.
Masuoka, Shinsuke
Baba, Hikari
Yoshikawa, Yuko
Prado-Gotor, Rafael
Yoshikawa, Kenichi
author_sort Carnerero, Jose M.
collection PubMed
description We decorated a single giant DNA (1.66 × 10(5) base pairs) with gold nanoparticles through the simple procedure of mild warming, without denaturation of the DNA molecule. Single-molecule observation with fluorescence microscopy revealed that individual decorated DNA molecules stay in the bulk solution by avoiding aggregation and precipitation, and exhibit translational and conformational fluctuation, i.e., Brownian motion. An analysis of the intra-chain fluctuation of single DNA molecules revealed that the apparent spring constant and damping coefficient of a DNA chain increased by ca. 13- and 5-fold, respectively, upon decoration with gold nanoparticles. Observation by transmission electron microscopy revealed that gold nanoparticles were stably attached to the DNA skeleton. UV-visible measurements revealed the absence of any detectable change in surface plasmon resonance, suggesting that the gold nanoparticles assemble without the formation of a densely packed aggregate. CD measurements showed that the secondary structure of decorated DNA is still essentially the B-form.
format Online
Article
Text
id pubmed-9083275
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90832752022-05-09 Decorating a single giant DNA with gold nanoparticles Carnerero, Jose M. Masuoka, Shinsuke Baba, Hikari Yoshikawa, Yuko Prado-Gotor, Rafael Yoshikawa, Kenichi RSC Adv Chemistry We decorated a single giant DNA (1.66 × 10(5) base pairs) with gold nanoparticles through the simple procedure of mild warming, without denaturation of the DNA molecule. Single-molecule observation with fluorescence microscopy revealed that individual decorated DNA molecules stay in the bulk solution by avoiding aggregation and precipitation, and exhibit translational and conformational fluctuation, i.e., Brownian motion. An analysis of the intra-chain fluctuation of single DNA molecules revealed that the apparent spring constant and damping coefficient of a DNA chain increased by ca. 13- and 5-fold, respectively, upon decoration with gold nanoparticles. Observation by transmission electron microscopy revealed that gold nanoparticles were stably attached to the DNA skeleton. UV-visible measurements revealed the absence of any detectable change in surface plasmon resonance, suggesting that the gold nanoparticles assemble without the formation of a densely packed aggregate. CD measurements showed that the secondary structure of decorated DNA is still essentially the B-form. The Royal Society of Chemistry 2018-07-25 /pmc/articles/PMC9083275/ /pubmed/35541036 http://dx.doi.org/10.1039/c8ra05088k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Carnerero, Jose M.
Masuoka, Shinsuke
Baba, Hikari
Yoshikawa, Yuko
Prado-Gotor, Rafael
Yoshikawa, Kenichi
Decorating a single giant DNA with gold nanoparticles
title Decorating a single giant DNA with gold nanoparticles
title_full Decorating a single giant DNA with gold nanoparticles
title_fullStr Decorating a single giant DNA with gold nanoparticles
title_full_unstemmed Decorating a single giant DNA with gold nanoparticles
title_short Decorating a single giant DNA with gold nanoparticles
title_sort decorating a single giant dna with gold nanoparticles
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083275/
https://www.ncbi.nlm.nih.gov/pubmed/35541036
http://dx.doi.org/10.1039/c8ra05088k
work_keys_str_mv AT carnererojosem decoratingasinglegiantdnawithgoldnanoparticles
AT masuokashinsuke decoratingasinglegiantdnawithgoldnanoparticles
AT babahikari decoratingasinglegiantdnawithgoldnanoparticles
AT yoshikawayuko decoratingasinglegiantdnawithgoldnanoparticles
AT pradogotorrafael decoratingasinglegiantdnawithgoldnanoparticles
AT yoshikawakenichi decoratingasinglegiantdnawithgoldnanoparticles