Cargando…
Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses
Tubby-like proteins (TLPs) are transcription factors that are widely present in eukaryotes and generally participate in growth and developmental processes. Using genome databases, a total of 22 putative TLP genes were identified in the soybean genome, and unevenly distributed across 13 chromosomes....
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083326/ https://www.ncbi.nlm.nih.gov/pubmed/35548296 http://dx.doi.org/10.3389/fpls.2022.844545 |
_version_ | 1784703397093441536 |
---|---|
author | Xu, Hong-Ru Liu, Ying Yu, Tai-Fei Hou, Ze-Hao Zheng, Jia-Cheng Chen, Jun Zhou, Yong-Bin Chen, Ming Fu, Jin-Dong Ma, You-Zhi Wei, Wen-Liang Xu, Zhao-Shi |
author_facet | Xu, Hong-Ru Liu, Ying Yu, Tai-Fei Hou, Ze-Hao Zheng, Jia-Cheng Chen, Jun Zhou, Yong-Bin Chen, Ming Fu, Jin-Dong Ma, You-Zhi Wei, Wen-Liang Xu, Zhao-Shi |
author_sort | Xu, Hong-Ru |
collection | PubMed |
description | Tubby-like proteins (TLPs) are transcription factors that are widely present in eukaryotes and generally participate in growth and developmental processes. Using genome databases, a total of 22 putative TLP genes were identified in the soybean genome, and unevenly distributed across 13 chromosomes. Phylogenetic analysis demonstrated that the predicted GmTLP proteins were divided into five groups (I-V). Gene structure, protein motifs, and conserved domains were analyzed to identify differences and common features among the GmTLPs. A three-dimensional protein model was built to show the typical structure of TLPs. Analysis of publicly available gene expression data showed that GmTLP genes were differentially expressed in response to abiotic stresses. Based on those data, GmTLP8 was selected to further explore the role of TLPs in soybean drought and salt stress responses. GmTLP8 overexpressors had improved tolerance to drought and salt stresses, whereas the opposite was true of GmTLP8-RNAi lines. 3,3-diaminobenzidine and nitro blue tetrazolium staining and physiological indexes also showed that overexpression of GmTLP8 enhanced the tolerance of soybean to drought and salt stresses; in addition, downstream stress-responsive genes were upregulated in response to drought and salt stresses. This study provides new insights into the function of GmTLPs in response to abiotic stresses. |
format | Online Article Text |
id | pubmed-9083326 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90833262022-05-10 Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses Xu, Hong-Ru Liu, Ying Yu, Tai-Fei Hou, Ze-Hao Zheng, Jia-Cheng Chen, Jun Zhou, Yong-Bin Chen, Ming Fu, Jin-Dong Ma, You-Zhi Wei, Wen-Liang Xu, Zhao-Shi Front Plant Sci Plant Science Tubby-like proteins (TLPs) are transcription factors that are widely present in eukaryotes and generally participate in growth and developmental processes. Using genome databases, a total of 22 putative TLP genes were identified in the soybean genome, and unevenly distributed across 13 chromosomes. Phylogenetic analysis demonstrated that the predicted GmTLP proteins were divided into five groups (I-V). Gene structure, protein motifs, and conserved domains were analyzed to identify differences and common features among the GmTLPs. A three-dimensional protein model was built to show the typical structure of TLPs. Analysis of publicly available gene expression data showed that GmTLP genes were differentially expressed in response to abiotic stresses. Based on those data, GmTLP8 was selected to further explore the role of TLPs in soybean drought and salt stress responses. GmTLP8 overexpressors had improved tolerance to drought and salt stresses, whereas the opposite was true of GmTLP8-RNAi lines. 3,3-diaminobenzidine and nitro blue tetrazolium staining and physiological indexes also showed that overexpression of GmTLP8 enhanced the tolerance of soybean to drought and salt stresses; in addition, downstream stress-responsive genes were upregulated in response to drought and salt stresses. This study provides new insights into the function of GmTLPs in response to abiotic stresses. Frontiers Media S.A. 2022-04-25 /pmc/articles/PMC9083326/ /pubmed/35548296 http://dx.doi.org/10.3389/fpls.2022.844545 Text en Copyright © 2022 Xu, Liu, Yu, Hou, Zheng, Chen, Zhou, Chen, Fu, Ma, Wei and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Xu, Hong-Ru Liu, Ying Yu, Tai-Fei Hou, Ze-Hao Zheng, Jia-Cheng Chen, Jun Zhou, Yong-Bin Chen, Ming Fu, Jin-Dong Ma, You-Zhi Wei, Wen-Liang Xu, Zhao-Shi Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses |
title | Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses |
title_full | Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses |
title_fullStr | Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses |
title_full_unstemmed | Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses |
title_short | Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses |
title_sort | comprehensive profiling of tubby-like proteins in soybean and roles of the gmtlp8 gene in abiotic stress responses |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083326/ https://www.ncbi.nlm.nih.gov/pubmed/35548296 http://dx.doi.org/10.3389/fpls.2022.844545 |
work_keys_str_mv | AT xuhongru comprehensiveprofilingoftubbylikeproteinsinsoybeanandrolesofthegmtlp8geneinabioticstressresponses AT liuying comprehensiveprofilingoftubbylikeproteinsinsoybeanandrolesofthegmtlp8geneinabioticstressresponses AT yutaifei comprehensiveprofilingoftubbylikeproteinsinsoybeanandrolesofthegmtlp8geneinabioticstressresponses AT houzehao comprehensiveprofilingoftubbylikeproteinsinsoybeanandrolesofthegmtlp8geneinabioticstressresponses AT zhengjiacheng comprehensiveprofilingoftubbylikeproteinsinsoybeanandrolesofthegmtlp8geneinabioticstressresponses AT chenjun comprehensiveprofilingoftubbylikeproteinsinsoybeanandrolesofthegmtlp8geneinabioticstressresponses AT zhouyongbin comprehensiveprofilingoftubbylikeproteinsinsoybeanandrolesofthegmtlp8geneinabioticstressresponses AT chenming comprehensiveprofilingoftubbylikeproteinsinsoybeanandrolesofthegmtlp8geneinabioticstressresponses AT fujindong comprehensiveprofilingoftubbylikeproteinsinsoybeanandrolesofthegmtlp8geneinabioticstressresponses AT mayouzhi comprehensiveprofilingoftubbylikeproteinsinsoybeanandrolesofthegmtlp8geneinabioticstressresponses AT weiwenliang comprehensiveprofilingoftubbylikeproteinsinsoybeanandrolesofthegmtlp8geneinabioticstressresponses AT xuzhaoshi comprehensiveprofilingoftubbylikeproteinsinsoybeanandrolesofthegmtlp8geneinabioticstressresponses |