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AtRsmD Is Required for Chloroplast Development and Chloroplast Function in Arabidopsis thaliana

AtRsmD was recently demonstrated to be a chloroplast 16S rRNA methyltransferase (MTase) for the m(2)G915 modification in Arabidopsis. Here, its function of AtRsmD for chloroplast development and photosynthesis was further analyzed. The AtRsmD gene is highly expressed in green photosynthetic tissues....

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Autores principales: Wang, Zi-Yuan, Qu, Wan-Tong, Mei, Tong, Zhang, Nan, Yang, Nai-Ying, Xu, Xiao-Feng, Xiong, Hai-Bo, Yang, Zhong-Nan, Yu, Qing-Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083416/
https://www.ncbi.nlm.nih.gov/pubmed/35548310
http://dx.doi.org/10.3389/fpls.2022.860945
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author Wang, Zi-Yuan
Qu, Wan-Tong
Mei, Tong
Zhang, Nan
Yang, Nai-Ying
Xu, Xiao-Feng
Xiong, Hai-Bo
Yang, Zhong-Nan
Yu, Qing-Bo
author_facet Wang, Zi-Yuan
Qu, Wan-Tong
Mei, Tong
Zhang, Nan
Yang, Nai-Ying
Xu, Xiao-Feng
Xiong, Hai-Bo
Yang, Zhong-Nan
Yu, Qing-Bo
author_sort Wang, Zi-Yuan
collection PubMed
description AtRsmD was recently demonstrated to be a chloroplast 16S rRNA methyltransferase (MTase) for the m(2)G915 modification in Arabidopsis. Here, its function of AtRsmD for chloroplast development and photosynthesis was further analyzed. The AtRsmD gene is highly expressed in green photosynthetic tissues. AtRsmD is associated with the thylakoid in chloroplasts. The atrsmd-2 mutant exhibited impaired photosynthetic efficiency in emerging leaves under normal growth conditions. A few thylakoid lamellas could be observed in the chloroplast from the atrsmd-2 mutant, and these thylakoids were loosely organized. Knockout of the AtRsmD gene had minor effects on chloroplast ribosome biogenesis and RNA loading on chloroplast ribosomes, but it reduced the amounts of chloroplast-encoded photosynthesis-related proteins in the emerging leaves, for example, D1, D2, CP43, and CP47, which reduced the accumulation of the photosynthetic complex. Nevertheless, knockout of the AtRsmD gene did not cause a general reduction in chloroplast-encoded proteins in Arabidopsis grown under normal growth conditions. Additionally, the atrsmd-2 mutant exhibited more sensitivity to lincomycin, which specifically inhibits the elongation of nascent polypeptide chains. Cold stress exacerbated the effect on chloroplast ribosome biogenesis in the atrsmd-2 mutant. All these data suggest that the AtRsmD protein plays distinct regulatory roles in chloroplast translation, which is required for chloroplast development and chloroplast function.
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spelling pubmed-90834162022-05-10 AtRsmD Is Required for Chloroplast Development and Chloroplast Function in Arabidopsis thaliana Wang, Zi-Yuan Qu, Wan-Tong Mei, Tong Zhang, Nan Yang, Nai-Ying Xu, Xiao-Feng Xiong, Hai-Bo Yang, Zhong-Nan Yu, Qing-Bo Front Plant Sci Plant Science AtRsmD was recently demonstrated to be a chloroplast 16S rRNA methyltransferase (MTase) for the m(2)G915 modification in Arabidopsis. Here, its function of AtRsmD for chloroplast development and photosynthesis was further analyzed. The AtRsmD gene is highly expressed in green photosynthetic tissues. AtRsmD is associated with the thylakoid in chloroplasts. The atrsmd-2 mutant exhibited impaired photosynthetic efficiency in emerging leaves under normal growth conditions. A few thylakoid lamellas could be observed in the chloroplast from the atrsmd-2 mutant, and these thylakoids were loosely organized. Knockout of the AtRsmD gene had minor effects on chloroplast ribosome biogenesis and RNA loading on chloroplast ribosomes, but it reduced the amounts of chloroplast-encoded photosynthesis-related proteins in the emerging leaves, for example, D1, D2, CP43, and CP47, which reduced the accumulation of the photosynthetic complex. Nevertheless, knockout of the AtRsmD gene did not cause a general reduction in chloroplast-encoded proteins in Arabidopsis grown under normal growth conditions. Additionally, the atrsmd-2 mutant exhibited more sensitivity to lincomycin, which specifically inhibits the elongation of nascent polypeptide chains. Cold stress exacerbated the effect on chloroplast ribosome biogenesis in the atrsmd-2 mutant. All these data suggest that the AtRsmD protein plays distinct regulatory roles in chloroplast translation, which is required for chloroplast development and chloroplast function. Frontiers Media S.A. 2022-04-25 /pmc/articles/PMC9083416/ /pubmed/35548310 http://dx.doi.org/10.3389/fpls.2022.860945 Text en Copyright © 2022 Wang, Qu, Mei, Zhang, Yang, Xu, Xiong, Yang and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Zi-Yuan
Qu, Wan-Tong
Mei, Tong
Zhang, Nan
Yang, Nai-Ying
Xu, Xiao-Feng
Xiong, Hai-Bo
Yang, Zhong-Nan
Yu, Qing-Bo
AtRsmD Is Required for Chloroplast Development and Chloroplast Function in Arabidopsis thaliana
title AtRsmD Is Required for Chloroplast Development and Chloroplast Function in Arabidopsis thaliana
title_full AtRsmD Is Required for Chloroplast Development and Chloroplast Function in Arabidopsis thaliana
title_fullStr AtRsmD Is Required for Chloroplast Development and Chloroplast Function in Arabidopsis thaliana
title_full_unstemmed AtRsmD Is Required for Chloroplast Development and Chloroplast Function in Arabidopsis thaliana
title_short AtRsmD Is Required for Chloroplast Development and Chloroplast Function in Arabidopsis thaliana
title_sort atrsmd is required for chloroplast development and chloroplast function in arabidopsis thaliana
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083416/
https://www.ncbi.nlm.nih.gov/pubmed/35548310
http://dx.doi.org/10.3389/fpls.2022.860945
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