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Acetylcholinesterase-functionalized two-dimensional photonic crystals for the detection of organophosphates
Due to the indiscriminate usage of organophosphates in military and agriculture, there is an increasing demand to develop sensors for monitoring organophosphorus nerve agents and pesticides. Herein, a sensitive two-dimensional photonic crystal (2D-PC) biosensor as a part of a portable device for the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9084495/ https://www.ncbi.nlm.nih.gov/pubmed/35548014 http://dx.doi.org/10.1039/c8ra04953j |
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author | Qi, Fenglian Lan, Yunhe Meng, Zihui Yan, Chunxiao Li, Shuguang Xue, Min Wang, Yifei Qiu, Lili He, Xuan Liu, Xueyong |
author_facet | Qi, Fenglian Lan, Yunhe Meng, Zihui Yan, Chunxiao Li, Shuguang Xue, Min Wang, Yifei Qiu, Lili He, Xuan Liu, Xueyong |
author_sort | Qi, Fenglian |
collection | PubMed |
description | Due to the indiscriminate usage of organophosphates in military and agriculture, there is an increasing demand to develop sensors for monitoring organophosphorus nerve agents and pesticides. Herein, a sensitive two-dimensional photonic crystal (2D-PC) biosensor as a part of a portable device for the detection of Dipterex is presented. The 2D-PC array was self-organized on the water–air interface using 600 nm polystyrene (PS) colloidal particles, and it was further embedded into a polyacrylamide-acrylic acid (PAM-AA) hydrogel. After condensation between the amine groups of acetylcholinesterase (AChE) and the carboxyl groups of the hydrogel, a novel sensing platform of 2D-PC hydrogel with AChE as the recognition agent was constructed. The lattice spacing of AChE-functionalized 2D-PC was inversely proportional to the logarithm of the Dipterex concentration from 10(−14) mol L(−1) to 10(−4) mol L(−1) with estimated limit of detection (LOD) of 7.7 × 10(−15) mol L(−1). Simultaneously, the structural color of AChE-functionalized 2D-PC varied from yellow to blue. The biosensor achieved “naked-eye” detection due to which the use of AChE-functionalized 2D-PC is a promising technique for onsite and fast screening of organophosphates. |
format | Online Article Text |
id | pubmed-9084495 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90844952022-05-10 Acetylcholinesterase-functionalized two-dimensional photonic crystals for the detection of organophosphates Qi, Fenglian Lan, Yunhe Meng, Zihui Yan, Chunxiao Li, Shuguang Xue, Min Wang, Yifei Qiu, Lili He, Xuan Liu, Xueyong RSC Adv Chemistry Due to the indiscriminate usage of organophosphates in military and agriculture, there is an increasing demand to develop sensors for monitoring organophosphorus nerve agents and pesticides. Herein, a sensitive two-dimensional photonic crystal (2D-PC) biosensor as a part of a portable device for the detection of Dipterex is presented. The 2D-PC array was self-organized on the water–air interface using 600 nm polystyrene (PS) colloidal particles, and it was further embedded into a polyacrylamide-acrylic acid (PAM-AA) hydrogel. After condensation between the amine groups of acetylcholinesterase (AChE) and the carboxyl groups of the hydrogel, a novel sensing platform of 2D-PC hydrogel with AChE as the recognition agent was constructed. The lattice spacing of AChE-functionalized 2D-PC was inversely proportional to the logarithm of the Dipterex concentration from 10(−14) mol L(−1) to 10(−4) mol L(−1) with estimated limit of detection (LOD) of 7.7 × 10(−15) mol L(−1). Simultaneously, the structural color of AChE-functionalized 2D-PC varied from yellow to blue. The biosensor achieved “naked-eye” detection due to which the use of AChE-functionalized 2D-PC is a promising technique for onsite and fast screening of organophosphates. The Royal Society of Chemistry 2018-08-20 /pmc/articles/PMC9084495/ /pubmed/35548014 http://dx.doi.org/10.1039/c8ra04953j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Qi, Fenglian Lan, Yunhe Meng, Zihui Yan, Chunxiao Li, Shuguang Xue, Min Wang, Yifei Qiu, Lili He, Xuan Liu, Xueyong Acetylcholinesterase-functionalized two-dimensional photonic crystals for the detection of organophosphates |
title | Acetylcholinesterase-functionalized two-dimensional photonic crystals for the detection of organophosphates |
title_full | Acetylcholinesterase-functionalized two-dimensional photonic crystals for the detection of organophosphates |
title_fullStr | Acetylcholinesterase-functionalized two-dimensional photonic crystals for the detection of organophosphates |
title_full_unstemmed | Acetylcholinesterase-functionalized two-dimensional photonic crystals for the detection of organophosphates |
title_short | Acetylcholinesterase-functionalized two-dimensional photonic crystals for the detection of organophosphates |
title_sort | acetylcholinesterase-functionalized two-dimensional photonic crystals for the detection of organophosphates |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9084495/ https://www.ncbi.nlm.nih.gov/pubmed/35548014 http://dx.doi.org/10.1039/c8ra04953j |
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