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Activated polymorphonuclear derived extracellular vesicles are potential biomarkers of periprosthetic joint infection

BACKGROUND: Extracellular vesicles (EVs) are considered as crucial players in a wide variety of biological processes. Although their importance in joint diseases or infections has been shown by numerous studies, much less is known about their function in periprosthetic joint infection (PJI). Our aim...

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Autores principales: Sallai, Imre, Marton, Nikolett, Szatmári, Attila, Kittel, Ágnes, Nagy, György, Buzás, Edit I., Khamari, Delaram, Komlósi, Zsolt, Kristóf, Katalin, Drahos, László, Turiák, Lilla, Sugár, Simon, Veres, Dániel Sándor, Kendoff, Daniel, Zahár, Ákos, Skaliczki, Gábor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9084519/
https://www.ncbi.nlm.nih.gov/pubmed/35533148
http://dx.doi.org/10.1371/journal.pone.0268076
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author Sallai, Imre
Marton, Nikolett
Szatmári, Attila
Kittel, Ágnes
Nagy, György
Buzás, Edit I.
Khamari, Delaram
Komlósi, Zsolt
Kristóf, Katalin
Drahos, László
Turiák, Lilla
Sugár, Simon
Veres, Dániel Sándor
Kendoff, Daniel
Zahár, Ákos
Skaliczki, Gábor
author_facet Sallai, Imre
Marton, Nikolett
Szatmári, Attila
Kittel, Ágnes
Nagy, György
Buzás, Edit I.
Khamari, Delaram
Komlósi, Zsolt
Kristóf, Katalin
Drahos, László
Turiák, Lilla
Sugár, Simon
Veres, Dániel Sándor
Kendoff, Daniel
Zahár, Ákos
Skaliczki, Gábor
author_sort Sallai, Imre
collection PubMed
description BACKGROUND: Extracellular vesicles (EVs) are considered as crucial players in a wide variety of biological processes. Although their importance in joint diseases or infections has been shown by numerous studies, much less is known about their function in periprosthetic joint infection (PJI). Our aim was to investigate activated polymorphonuclear (PMN)-derived synovial EVs in patients with PJI. QUESTIONS/PURPOSES: (1) Is there a difference in the number and size of extracellular vesicles between periprosthetic joint aspirates of patients with PJI and aseptic loosening? (2) Are these vesicles morphologically different in the two groups? (3) Are there activated PMN-derived EVs in septic samples evaluated by flow cytometry after CD177 labelling? (4) Is there a difference in the protein composition carried by septic and aseptic vesicles? METHODS: Thirty-four patients (n = 34) were enrolled into our investigation, 17 with PJI and 17 with aseptic prosthesis loosening. Periprosthetic joint fluid was aspirated and EVs were separated. Samples were analysed by nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) and flow cytometry (after Annexin V and CD177 labelling). The protein content of the EVs was studied by mass spectrometry (MS). RESULTS: NTA showed particle size distribution in both groups between 150 nm and 450 nm. The concentration of EVs was significantly higher in the septic samples (p = 0.0105) and showed a different size pattern as compared to the aseptic ones. The vesicular nature of the particles was confirmed by TEM and differential detergent lysis. In the septic group, FC analysis showed a significantly increased event number both after single and double labelling with fluorochrome conjugated Annexin V (p = 0.046) and Annexin V and anti-CD177 (p = 0.0105), respectively. MS detected a significant difference in the abundance of lactotransferrin (p = 0.00646), myeloperoxidase (p = 0.01061), lysozyme C (p = 0.04687), annexin A6 (p = 0.03921) and alpha-2-HS-glycoprotein (p = 0.03146) between the studied groups. CONCLUSIONS: An increased number of activated PMN derived EVs were detected in the synovial fluid of PJI patients with a characteristic size distribution and a specific protein composition. The activated PMNs-derived extracellular vesicles can be potential biomarkers of PJI.
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spelling pubmed-90845192022-05-10 Activated polymorphonuclear derived extracellular vesicles are potential biomarkers of periprosthetic joint infection Sallai, Imre Marton, Nikolett Szatmári, Attila Kittel, Ágnes Nagy, György Buzás, Edit I. Khamari, Delaram Komlósi, Zsolt Kristóf, Katalin Drahos, László Turiák, Lilla Sugár, Simon Veres, Dániel Sándor Kendoff, Daniel Zahár, Ákos Skaliczki, Gábor PLoS One Research Article BACKGROUND: Extracellular vesicles (EVs) are considered as crucial players in a wide variety of biological processes. Although their importance in joint diseases or infections has been shown by numerous studies, much less is known about their function in periprosthetic joint infection (PJI). Our aim was to investigate activated polymorphonuclear (PMN)-derived synovial EVs in patients with PJI. QUESTIONS/PURPOSES: (1) Is there a difference in the number and size of extracellular vesicles between periprosthetic joint aspirates of patients with PJI and aseptic loosening? (2) Are these vesicles morphologically different in the two groups? (3) Are there activated PMN-derived EVs in septic samples evaluated by flow cytometry after CD177 labelling? (4) Is there a difference in the protein composition carried by septic and aseptic vesicles? METHODS: Thirty-four patients (n = 34) were enrolled into our investigation, 17 with PJI and 17 with aseptic prosthesis loosening. Periprosthetic joint fluid was aspirated and EVs were separated. Samples were analysed by nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) and flow cytometry (after Annexin V and CD177 labelling). The protein content of the EVs was studied by mass spectrometry (MS). RESULTS: NTA showed particle size distribution in both groups between 150 nm and 450 nm. The concentration of EVs was significantly higher in the septic samples (p = 0.0105) and showed a different size pattern as compared to the aseptic ones. The vesicular nature of the particles was confirmed by TEM and differential detergent lysis. In the septic group, FC analysis showed a significantly increased event number both after single and double labelling with fluorochrome conjugated Annexin V (p = 0.046) and Annexin V and anti-CD177 (p = 0.0105), respectively. MS detected a significant difference in the abundance of lactotransferrin (p = 0.00646), myeloperoxidase (p = 0.01061), lysozyme C (p = 0.04687), annexin A6 (p = 0.03921) and alpha-2-HS-glycoprotein (p = 0.03146) between the studied groups. CONCLUSIONS: An increased number of activated PMN derived EVs were detected in the synovial fluid of PJI patients with a characteristic size distribution and a specific protein composition. The activated PMNs-derived extracellular vesicles can be potential biomarkers of PJI. Public Library of Science 2022-05-09 /pmc/articles/PMC9084519/ /pubmed/35533148 http://dx.doi.org/10.1371/journal.pone.0268076 Text en © 2022 Sallai et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sallai, Imre
Marton, Nikolett
Szatmári, Attila
Kittel, Ágnes
Nagy, György
Buzás, Edit I.
Khamari, Delaram
Komlósi, Zsolt
Kristóf, Katalin
Drahos, László
Turiák, Lilla
Sugár, Simon
Veres, Dániel Sándor
Kendoff, Daniel
Zahár, Ákos
Skaliczki, Gábor
Activated polymorphonuclear derived extracellular vesicles are potential biomarkers of periprosthetic joint infection
title Activated polymorphonuclear derived extracellular vesicles are potential biomarkers of periprosthetic joint infection
title_full Activated polymorphonuclear derived extracellular vesicles are potential biomarkers of periprosthetic joint infection
title_fullStr Activated polymorphonuclear derived extracellular vesicles are potential biomarkers of periprosthetic joint infection
title_full_unstemmed Activated polymorphonuclear derived extracellular vesicles are potential biomarkers of periprosthetic joint infection
title_short Activated polymorphonuclear derived extracellular vesicles are potential biomarkers of periprosthetic joint infection
title_sort activated polymorphonuclear derived extracellular vesicles are potential biomarkers of periprosthetic joint infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9084519/
https://www.ncbi.nlm.nih.gov/pubmed/35533148
http://dx.doi.org/10.1371/journal.pone.0268076
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