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Tanreqing Injection Attenuates Macrophage Activation and the Inflammatory Response via the lncRNA-SNHG1/HMGB1 Axis in Lipopolysaccharide-Induced Acute Lung Injury

The etiology of acute lung injury (ALI) is not clear, and the treatment of ALI presents a great challenge. This study aimed to investigate the pathogenesis and potential therapeutic targets of ALI and to define the target gene of Tanreqing (TRQ), which is a traditional Chinese medicine formula compo...

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Autores principales: Hu, Chunling, Li, Junlu, Tan, Yingshuai, Liu, Yang, Bai, Chen, Gao, Jing, Zhao, Shilong, Yao, Mengying, Lu, Xiaoxiao, Qiu, Lingxiao, Xing, Lihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9084914/
https://www.ncbi.nlm.nih.gov/pubmed/35547731
http://dx.doi.org/10.3389/fimmu.2022.820718
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author Hu, Chunling
Li, Junlu
Tan, Yingshuai
Liu, Yang
Bai, Chen
Gao, Jing
Zhao, Shilong
Yao, Mengying
Lu, Xiaoxiao
Qiu, Lingxiao
Xing, Lihua
author_facet Hu, Chunling
Li, Junlu
Tan, Yingshuai
Liu, Yang
Bai, Chen
Gao, Jing
Zhao, Shilong
Yao, Mengying
Lu, Xiaoxiao
Qiu, Lingxiao
Xing, Lihua
author_sort Hu, Chunling
collection PubMed
description The etiology of acute lung injury (ALI) is not clear, and the treatment of ALI presents a great challenge. This study aimed to investigate the pathogenesis and potential therapeutic targets of ALI and to define the target gene of Tanreqing (TRQ), which is a traditional Chinese medicine formula composed of five medicines, scutellaria baicalensis, bear bile powder, goat horn powder, honeysuckle and forsythia. Macrophage activation plays a critical role in many pathophysiological processes, such as inflammation. Although the regulation of macrophage activation has been extensively investigated, there is little knowledge of the role of long noncoding RNAs (lncRNAs) in this process. In this study, we found that lncRNA-SNHG1 expression is distinctly regulated in differently activated macrophages in that it is upregulated in LPS. LncRNA-SNHG1 knockdown attenuates LPS-induced M1 macrophage activation. The SNHG1 promoter was bound by NF-κB subunit p65, indicative of SNHG1 being a direct transcriptional target of LPS-induced NF-κB activation. SNHG1 acts as a proinflammatory driver that leads to the production of inflammatory cytokines and the activation of macrophages and cytokine storms by physically interacting with high-mobility group box 1 (HMGB1) in ALI. TRQ inhibited NF-κB signaling activation and binding of NF-κB to the SNHG1 promoter. In conclusion, this study defined TRQ target genes, which can be further elucidated as mechanism(s) of TRQ action, and provides insight into the molecular pathogenesis of ALI. The lncRNA-SNHG1/HMGB1 axis is an ideal therapeutic for ALI treatment.
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spelling pubmed-90849142022-05-10 Tanreqing Injection Attenuates Macrophage Activation and the Inflammatory Response via the lncRNA-SNHG1/HMGB1 Axis in Lipopolysaccharide-Induced Acute Lung Injury Hu, Chunling Li, Junlu Tan, Yingshuai Liu, Yang Bai, Chen Gao, Jing Zhao, Shilong Yao, Mengying Lu, Xiaoxiao Qiu, Lingxiao Xing, Lihua Front Immunol Immunology The etiology of acute lung injury (ALI) is not clear, and the treatment of ALI presents a great challenge. This study aimed to investigate the pathogenesis and potential therapeutic targets of ALI and to define the target gene of Tanreqing (TRQ), which is a traditional Chinese medicine formula composed of five medicines, scutellaria baicalensis, bear bile powder, goat horn powder, honeysuckle and forsythia. Macrophage activation plays a critical role in many pathophysiological processes, such as inflammation. Although the regulation of macrophage activation has been extensively investigated, there is little knowledge of the role of long noncoding RNAs (lncRNAs) in this process. In this study, we found that lncRNA-SNHG1 expression is distinctly regulated in differently activated macrophages in that it is upregulated in LPS. LncRNA-SNHG1 knockdown attenuates LPS-induced M1 macrophage activation. The SNHG1 promoter was bound by NF-κB subunit p65, indicative of SNHG1 being a direct transcriptional target of LPS-induced NF-κB activation. SNHG1 acts as a proinflammatory driver that leads to the production of inflammatory cytokines and the activation of macrophages and cytokine storms by physically interacting with high-mobility group box 1 (HMGB1) in ALI. TRQ inhibited NF-κB signaling activation and binding of NF-κB to the SNHG1 promoter. In conclusion, this study defined TRQ target genes, which can be further elucidated as mechanism(s) of TRQ action, and provides insight into the molecular pathogenesis of ALI. The lncRNA-SNHG1/HMGB1 axis is an ideal therapeutic for ALI treatment. Frontiers Media S.A. 2022-04-25 /pmc/articles/PMC9084914/ /pubmed/35547731 http://dx.doi.org/10.3389/fimmu.2022.820718 Text en Copyright © 2022 Hu, Li, Tan, Liu, Bai, Gao, Zhao, Yao, Lu, Qiu and Xing https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Hu, Chunling
Li, Junlu
Tan, Yingshuai
Liu, Yang
Bai, Chen
Gao, Jing
Zhao, Shilong
Yao, Mengying
Lu, Xiaoxiao
Qiu, Lingxiao
Xing, Lihua
Tanreqing Injection Attenuates Macrophage Activation and the Inflammatory Response via the lncRNA-SNHG1/HMGB1 Axis in Lipopolysaccharide-Induced Acute Lung Injury
title Tanreqing Injection Attenuates Macrophage Activation and the Inflammatory Response via the lncRNA-SNHG1/HMGB1 Axis in Lipopolysaccharide-Induced Acute Lung Injury
title_full Tanreqing Injection Attenuates Macrophage Activation and the Inflammatory Response via the lncRNA-SNHG1/HMGB1 Axis in Lipopolysaccharide-Induced Acute Lung Injury
title_fullStr Tanreqing Injection Attenuates Macrophage Activation and the Inflammatory Response via the lncRNA-SNHG1/HMGB1 Axis in Lipopolysaccharide-Induced Acute Lung Injury
title_full_unstemmed Tanreqing Injection Attenuates Macrophage Activation and the Inflammatory Response via the lncRNA-SNHG1/HMGB1 Axis in Lipopolysaccharide-Induced Acute Lung Injury
title_short Tanreqing Injection Attenuates Macrophage Activation and the Inflammatory Response via the lncRNA-SNHG1/HMGB1 Axis in Lipopolysaccharide-Induced Acute Lung Injury
title_sort tanreqing injection attenuates macrophage activation and the inflammatory response via the lncrna-snhg1/hmgb1 axis in lipopolysaccharide-induced acute lung injury
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9084914/
https://www.ncbi.nlm.nih.gov/pubmed/35547731
http://dx.doi.org/10.3389/fimmu.2022.820718
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