Cargando…

SARS-CoV-2 RNA detection in Formalin-Fixed Paraffin-Embedded (FFPE) tissue by droplet digital PCR (ddPCR)

BACKGROUND: SARS-CoV-2 is an RNA virus that primarily causes respiratory disease; however, infection of other tissue has been reported. Evaluation of SARS-CoV-2 in tissue specimens may increase understanding of SARS-CoV-2 pathobiology. MATERIALS AND METHODS: A qualitative test for detection of SARS-...

Descripción completa

Detalles Bibliográficos
Autores principales:	Majumdar, Ramanath, Vrana, Julie A., Koepplin, Justin W., Milosevic, Dragana, Roden, Anja C., Garcia, Joaquin J., Kipp, Benjamin R., Moyer, Ann M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier B.V. 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085371/ https://www.ncbi.nlm.nih.gov/pubmed/35550815 http://dx.doi.org/10.1016/j.cca.2022.05.007

Ejemplares similares

Accuracy and Clinical Relevance of Intra-Tumoral Fusobacterium nucleatum Detection in Formalin-Fixed Paraffin-Embedded (FFPE) Tissue by Droplet Digital PCR (ddPCR) in Colorectal Cancer
por: Datorre, José Guilherme, et al.
Publicado: (2022)

Using ddPCR to assess the DNA yield of FFPE samples
por: Lu, X.J. David, et al.
Publicado: (2018)

Droplet digital PCR (ddPCR) for the detection and quantification of Ureaplasma spp.
por: Huang, Yanfang, et al.
Publicado: (2021)

Machine learning algorithm improved automated droplet classification of ddPCR for detection of BRAF V600E in paraffin-embedded samples
por: Colozza-Gama, Gabriel A., et al.
Publicado: (2021)

Maximizing viral detection with SIV droplet digital PCR (ddPCR) assays
por: Long, Samuel, et al.
Publicado: (2020)

Author Correction: Machine learning algorithm improved automated droplet classification of ddPCR for detection of BRAF V600E in paraffin-embedded samples
por: Colozza-Gama, Gabriel A., et al.
Publicado: (2021)

Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)
por: Koepfli, Cristian, et al.
Publicado: (2016)

Ultrasensitive detection and quantification of viral nucleic acids with Raindance droplet digital PCR (ddPCR)
por: Long, Samuel, et al.
Publicado: (2022)

A reverse-transcription droplet digital PCR assay to detect and quantify SARS-CoV-2 RNA in upper respiratory tract specimens
por: Milosevic, Dragana, et al.
Publicado: (2022)

Development and optimization of a simian immunodeficiency virus (SIV) droplet digital PCR (ddPCR) assay
por: Long, Samuel, et al.
Publicado: (2020)

Droplet Digital PCR (ddPCR) Analysis for the Detection and Quantification of Cow DNA in Buffalo Mozzarella Cheese
por: Cutarelli, Anna, et al.
Publicado: (2021)

Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue
por: Robertson, David, et al.
Publicado: (2008)

Toward deciphering proteomes of formalin-fixed paraffin-embedded (FFPE) tissues
por: Magdeldin, Sameh, et al.
Publicado: (2012)

Droplet digital polymerase chain reaction (ddPCR) for the detection of Plasmodium knowlesi and Plasmodium vivax
por: Mahendran, Punitha, et al.
Publicado: (2020)

Respiratory Adenovirus Quantification with a Droplet Digital Polymerase Chain Reaction (ddPCR) Assay
por: Abdullah, Omar, et al.
Publicado: (2023)

A droplet digital PCR (ddPCR) assay to detect Helicoverpa armigera (Lepidoptera: Noctuidae) in bulk trap samples
por: Zink, Frida A., et al.
Publicado: (2017)

Digital droplet PCR (ddPCR) for the detection and quantification of HPV 16, 18, 33 and 45 - a short report
por: Lillsunde Larsson, Gabriella, et al.
Publicado: (2017)

Diagnostic, Prognostic, and Therapeutic Value of Droplet Digital PCR (ddPCR) in COVID-19 Patients: A Systematic Review
por: Ishak, Angela, et al.
Publicado: (2021)

Putative Clinical Potential of ERBB2 Amplification Assessment by ddPCR in FFPE-DNA and cfDNA of Gastroesophageal Adenocarcinoma Patients
por: Boldrin, Elisa, et al.
Publicado: (2022)

Somatic Mutation Allelic Ratio Test Using ddPCR (SMART-ddPCR): An Accurate Method for Assessment of Preferential Allelic Imbalance in Tumor DNA
por: de Smith, Adam J., et al.
Publicado: (2015)

Rapid and accurate identification of SARS-CoV-2 Omicron variants using droplet digital PCR (RT-ddPCR)
por: Mills, Margaret G., et al.
Publicado: (2022)

Validation of a Droplet Digital PCR (ddPCR) Assay to Detect Cyanobacterial 16S rDNA in Human Lung Tissue
por: Barney, Rachael E., et al.
Publicado: (2023)

Correction: Droplet digital polymerase chain reaction (ddPCR) for the detection of Plasmodium knowlesi and Plasmodium vivax
por: Mahendran, Punitha, et al.
Publicado: (2023)

Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)
por: Long, Samuel, et al.
Publicado: (2021)

Quantitation and integrity evaluation of RNA genome in lentiviral vectors by direct reverse transcription-droplet digital PCR (direct RT-ddPCR)
por: He, Zhiyong, et al.
Publicado: (2023)

ddPCRclust: an R package and Shiny app for automated analysis of multiplexed ddPCR data
por: Brink, Benedikt G, et al.
Publicado: (2018)

Rapid, precise quantification of large DNA excisions and inversions by ddPCR
por: Watry, Hannah L., et al.
Publicado: (2020)

HIV reservoir quantification using cross-subtype multiplex ddPCR
por: Cassidy, Noah A.J., et al.
Publicado: (2021)

Evaluation of Droplet Digital Polymerase Chain Reaction (ddPCR) for the Absolute Quantification of Aspergillus species in the Human Airway
por: Poh, Tuang Yeow, et al.
Publicado: (2020)

A qPCR Method for AAV Genome Titer with ddPCR-Level of Accuracy and Precision
por: Wang, Yu, et al.
Publicado: (2020)

A Propidium Monoazide (PMAxx)-Droplet Digital PCR (ddPCR) for the Detection of Viable Burkholderia cepacia Complex in Nuclease-Free Water and Antiseptics
por: Daddy Gaoh, Soumana, et al.
Publicado: (2022)

Enhancing the compatibility of BioCaRGOS silica sol-gel technology with ctDNA extraction and droplet digital PCR (ddPCR) analysis
por: Potnis, Chinmay S., et al.
Publicado: (2022)

PCR for the Diagnosis of Abdominal Angiostrongyliasis in Formalin-Fixed Paraffin-Embedded Human Tissue
por: Rodriguez, Rubens, et al.
Publicado: (2014)

Transcriptome Profiling of Archived Sectioned Formalin-Fixed Paraffin-Embedded (AS-FFPE) Tissue for Disease Classification
por: Kojima, Kensuke, et al.
Publicado: (2014)

miRNA expression profiling of formalin-fixed paraffin-embedded (FFPE) hereditary breast tumors
por: Tanić, Miljana, et al.
Publicado: (2014)

Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
por: Park, Changwoo, et al.
Publicado: (2023)

Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics
por: Rose, John C., et al.
Publicado: (2017)

Standardization of the liquid biopsy for pediatric diffuse midline glioma using ddPCR
por: Li, Daphne, et al.
Publicado: (2021)

The Effectiveness of ddPCR for Detection of Point Mutations in Poor-Quality Saliva Samples
por: Riedlova, Petra, et al.
Publicado: (2022)

Identification of rare levels of methylated tumor DNA fragments using an optimized bias based pre-amplification-digital droplet PCR (OBBPA-ddPCR)
por: Menschikowski, Mario, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_fj8q17ll5ucmi7do3riv3v517c