Cargando…

Effects of riboflavin and AQS as electron shuttles on U(vi) reduction and precipitation by Shewanella putrefaciens

Understanding the mechanisms for electron shuttles (ESs) in microbial extracellular electron transfer (EET) is important in biogeochemical cycles, bioremediation applications, as well as bioenergy strategies. In this work, we adapted electrochemical techniques to probe electrochemically active and r...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Pingping, Dong, Faqin, Wang, Xuhui, Liu, Mingxue, Nie, Xiaoqin, Zhou, Lei, Huo, Tingting, Zhang, Wei, Wei, Hongfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085505/
https://www.ncbi.nlm.nih.gov/pubmed/35548745
http://dx.doi.org/10.1039/c8ra05715j
_version_ 1784703833915523072
author Wang, Pingping
Dong, Faqin
Wang, Xuhui
Liu, Mingxue
Nie, Xiaoqin
Zhou, Lei
Huo, Tingting
Zhang, Wei
Wei, Hongfu
author_facet Wang, Pingping
Dong, Faqin
Wang, Xuhui
Liu, Mingxue
Nie, Xiaoqin
Zhou, Lei
Huo, Tingting
Zhang, Wei
Wei, Hongfu
author_sort Wang, Pingping
collection PubMed
description Understanding the mechanisms for electron shuttles (ESs) in microbial extracellular electron transfer (EET) is important in biogeochemical cycles, bioremediation applications, as well as bioenergy strategies. In this work, we adapted electrochemical techniques to probe electrochemically active and redox-active Shewanella putrefaciens. This approach detected flavins and humic-like substances of Shewanella putrefaciens, which were involved in electron transfer to the electrode. A combination of three-dimensional excitation-emission (EEM) florescence spectroscopy methods identified a mixture of riboflavin and humic-like substances in supernatants during sustained incubations. The reductive behaviour of U(vi) by Shewanella putrefaciens in the presence of riboflavin (RF) and anthraquinone-2-sulfonate (AQS) was also investigated in this study. The results indicated that RF and AQS significantly accelerated electron transfer from cells to U(vi), thus enhancing reductive U(vi). The precipitate was further evidenced by SEM, FTIR, XPS and XRD, which demonstrated that chernikovite [H(2)(UO(2))(2)(PO(4))(2)·8H(2)O] became the main product on the cell surface of S. putrefaciens. In a contrast, U(iv) mainly existed amorphously on the cell surface of S. putrefaciens with added RF and AQS. This work has significant implications in elucidating RF and AQS as electron shuttles that are efficient in reduction of uranium in geological environments.
format Online
Article
Text
id pubmed-9085505
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90855052022-05-10 Effects of riboflavin and AQS as electron shuttles on U(vi) reduction and precipitation by Shewanella putrefaciens Wang, Pingping Dong, Faqin Wang, Xuhui Liu, Mingxue Nie, Xiaoqin Zhou, Lei Huo, Tingting Zhang, Wei Wei, Hongfu RSC Adv Chemistry Understanding the mechanisms for electron shuttles (ESs) in microbial extracellular electron transfer (EET) is important in biogeochemical cycles, bioremediation applications, as well as bioenergy strategies. In this work, we adapted electrochemical techniques to probe electrochemically active and redox-active Shewanella putrefaciens. This approach detected flavins and humic-like substances of Shewanella putrefaciens, which were involved in electron transfer to the electrode. A combination of three-dimensional excitation-emission (EEM) florescence spectroscopy methods identified a mixture of riboflavin and humic-like substances in supernatants during sustained incubations. The reductive behaviour of U(vi) by Shewanella putrefaciens in the presence of riboflavin (RF) and anthraquinone-2-sulfonate (AQS) was also investigated in this study. The results indicated that RF and AQS significantly accelerated electron transfer from cells to U(vi), thus enhancing reductive U(vi). The precipitate was further evidenced by SEM, FTIR, XPS and XRD, which demonstrated that chernikovite [H(2)(UO(2))(2)(PO(4))(2)·8H(2)O] became the main product on the cell surface of S. putrefaciens. In a contrast, U(iv) mainly existed amorphously on the cell surface of S. putrefaciens with added RF and AQS. This work has significant implications in elucidating RF and AQS as electron shuttles that are efficient in reduction of uranium in geological environments. The Royal Society of Chemistry 2018-08-31 /pmc/articles/PMC9085505/ /pubmed/35548745 http://dx.doi.org/10.1039/c8ra05715j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Wang, Pingping
Dong, Faqin
Wang, Xuhui
Liu, Mingxue
Nie, Xiaoqin
Zhou, Lei
Huo, Tingting
Zhang, Wei
Wei, Hongfu
Effects of riboflavin and AQS as electron shuttles on U(vi) reduction and precipitation by Shewanella putrefaciens
title Effects of riboflavin and AQS as electron shuttles on U(vi) reduction and precipitation by Shewanella putrefaciens
title_full Effects of riboflavin and AQS as electron shuttles on U(vi) reduction and precipitation by Shewanella putrefaciens
title_fullStr Effects of riboflavin and AQS as electron shuttles on U(vi) reduction and precipitation by Shewanella putrefaciens
title_full_unstemmed Effects of riboflavin and AQS as electron shuttles on U(vi) reduction and precipitation by Shewanella putrefaciens
title_short Effects of riboflavin and AQS as electron shuttles on U(vi) reduction and precipitation by Shewanella putrefaciens
title_sort effects of riboflavin and aqs as electron shuttles on u(vi) reduction and precipitation by shewanella putrefaciens
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085505/
https://www.ncbi.nlm.nih.gov/pubmed/35548745
http://dx.doi.org/10.1039/c8ra05715j
work_keys_str_mv AT wangpingping effectsofriboflavinandaqsaselectronshuttlesonuvireductionandprecipitationbyshewanellaputrefaciens
AT dongfaqin effectsofriboflavinandaqsaselectronshuttlesonuvireductionandprecipitationbyshewanellaputrefaciens
AT wangxuhui effectsofriboflavinandaqsaselectronshuttlesonuvireductionandprecipitationbyshewanellaputrefaciens
AT liumingxue effectsofriboflavinandaqsaselectronshuttlesonuvireductionandprecipitationbyshewanellaputrefaciens
AT niexiaoqin effectsofriboflavinandaqsaselectronshuttlesonuvireductionandprecipitationbyshewanellaputrefaciens
AT zhoulei effectsofriboflavinandaqsaselectronshuttlesonuvireductionandprecipitationbyshewanellaputrefaciens
AT huotingting effectsofriboflavinandaqsaselectronshuttlesonuvireductionandprecipitationbyshewanellaputrefaciens
AT zhangwei effectsofriboflavinandaqsaselectronshuttlesonuvireductionandprecipitationbyshewanellaputrefaciens
AT weihongfu effectsofriboflavinandaqsaselectronshuttlesonuvireductionandprecipitationbyshewanellaputrefaciens