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Investigation of human parvovirus B19 prevalence in a large healthy umbilical cord blood donors

BACKGROUND AND OBJECTIVES: Umbilical cord blood (UCB) was used to source hematopoietic stem cells in the past. Despite the apparent advantages of UCB transplantation, virus reactivation poses a considerable danger in allogeneic hematopoietic stem cell transplantation (HSCT). Human Parvovirus B19 is...

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Detalles Bibliográficos
Autores principales: Ramezany, Hooman, Kheirandish, Maryam, Samiee, Shahram, Khosravifar, Mina, Hashemi, Seyedeh Melika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085544/
https://www.ncbi.nlm.nih.gov/pubmed/35664722
http://dx.doi.org/10.18502/ijm.v14i1.8813
Descripción
Sumario:BACKGROUND AND OBJECTIVES: Umbilical cord blood (UCB) was used to source hematopoietic stem cells in the past. Despite the apparent advantages of UCB transplantation, virus reactivation poses a considerable danger in allogeneic hematopoietic stem cell transplantation (HSCT). Human Parvovirus B19 is regarded as a potential threat to UCB contamination. This study aimed to evaluate the prevalence of parvovirus B19 in cord blood donors by Semi-Nested PCR. This study is the first largescale report of the B19 DNA in cord blood donors in Iran. MATERIALS AND METHODS: A total of 691 umbilical cord blood were collected under standard procedure. Then, DNA from buffy coat and plasma were extracted, and semi-nested PCR was performed for all samples. RESULTS: Two out of 691 samples (0.29%) indicated viremia in plasma and buffy coat. CONCLUSION: In this line, designing and validating a quantitative PCR assay for detection, quantification, and discrimination of Human B19 DNA genotypes of cord blood donors is necessary to enhance the safety of this source of stem cells.