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Label-free and real-time monitoring of human mesenchymal stem cell differentiation in 2D and 3D cell culture systems using impedance cell sensors

Three dimensional (3D) stem cell culture has recently received considerable attention because it may enable the development of in vitro 3D tissue models. In particular, label-free and real-time monitoring of stem cell differentiation is of importance for tissue engineering applications; however, onl...

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Detalles Bibliográficos
Autores principales: Song, Jun Ho, Lee, Sun-Mi, Yoo, Kyung-Hwa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085567/
https://www.ncbi.nlm.nih.gov/pubmed/35548770
http://dx.doi.org/10.1039/c8ra05273e
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author Song, Jun Ho
Lee, Sun-Mi
Yoo, Kyung-Hwa
author_facet Song, Jun Ho
Lee, Sun-Mi
Yoo, Kyung-Hwa
author_sort Song, Jun Ho
collection PubMed
description Three dimensional (3D) stem cell culture has recently received considerable attention because it may enable the development of in vitro 3D tissue models. In particular, label-free and real-time monitoring of stem cell differentiation is of importance for tissue engineering applications; however, only a few non-invasive monitoring methods are available, especially for 3D cell culture. Here, we describe impedance cell sensors that allowed the monitoring of cellular behaviors in 2D and 3D cell cultures in real-time. Specifically, apparent capacitance peaks appeared in both 2D and 3D cell culture systems when human mesenchymal stem cells (hMSCs) were cultured in osteogenic induction medium. In contrast, when hMSCs were cultured in adipogenic induction medium, the capacitance increased monotonically. In addition, distinct characteristics were noted in the plots of capacitance versus conductance for the cells cultured in osteogenic and adipocyte induction media. These results demonstrated that the differentiation of hMSCs toward osteoblasts and adipocytes in 2D and 3D cell culture systems could be discriminated non-invasively by measuring the real-time capacitance and conductance. Furthermore, the vertical distribution of cellular activities in 3D cell cultures could be monitored in real-time using the 3D impedance cell sensors. Thus, these sensors may be suitable for monitoring the differentiation of various stem cells into different types of cells with distinct dielectric properties for tissue engineering applications.
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spelling pubmed-90855672022-05-10 Label-free and real-time monitoring of human mesenchymal stem cell differentiation in 2D and 3D cell culture systems using impedance cell sensors Song, Jun Ho Lee, Sun-Mi Yoo, Kyung-Hwa RSC Adv Chemistry Three dimensional (3D) stem cell culture has recently received considerable attention because it may enable the development of in vitro 3D tissue models. In particular, label-free and real-time monitoring of stem cell differentiation is of importance for tissue engineering applications; however, only a few non-invasive monitoring methods are available, especially for 3D cell culture. Here, we describe impedance cell sensors that allowed the monitoring of cellular behaviors in 2D and 3D cell cultures in real-time. Specifically, apparent capacitance peaks appeared in both 2D and 3D cell culture systems when human mesenchymal stem cells (hMSCs) were cultured in osteogenic induction medium. In contrast, when hMSCs were cultured in adipogenic induction medium, the capacitance increased monotonically. In addition, distinct characteristics were noted in the plots of capacitance versus conductance for the cells cultured in osteogenic and adipocyte induction media. These results demonstrated that the differentiation of hMSCs toward osteoblasts and adipocytes in 2D and 3D cell culture systems could be discriminated non-invasively by measuring the real-time capacitance and conductance. Furthermore, the vertical distribution of cellular activities in 3D cell cultures could be monitored in real-time using the 3D impedance cell sensors. Thus, these sensors may be suitable for monitoring the differentiation of various stem cells into different types of cells with distinct dielectric properties for tissue engineering applications. The Royal Society of Chemistry 2018-09-04 /pmc/articles/PMC9085567/ /pubmed/35548770 http://dx.doi.org/10.1039/c8ra05273e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Song, Jun Ho
Lee, Sun-Mi
Yoo, Kyung-Hwa
Label-free and real-time monitoring of human mesenchymal stem cell differentiation in 2D and 3D cell culture systems using impedance cell sensors
title Label-free and real-time monitoring of human mesenchymal stem cell differentiation in 2D and 3D cell culture systems using impedance cell sensors
title_full Label-free and real-time monitoring of human mesenchymal stem cell differentiation in 2D and 3D cell culture systems using impedance cell sensors
title_fullStr Label-free and real-time monitoring of human mesenchymal stem cell differentiation in 2D and 3D cell culture systems using impedance cell sensors
title_full_unstemmed Label-free and real-time monitoring of human mesenchymal stem cell differentiation in 2D and 3D cell culture systems using impedance cell sensors
title_short Label-free and real-time monitoring of human mesenchymal stem cell differentiation in 2D and 3D cell culture systems using impedance cell sensors
title_sort label-free and real-time monitoring of human mesenchymal stem cell differentiation in 2d and 3d cell culture systems using impedance cell sensors
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085567/
https://www.ncbi.nlm.nih.gov/pubmed/35548770
http://dx.doi.org/10.1039/c8ra05273e
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