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Targeting double-strand break indel byproducts with secondary guide RNAs improves Cas9 HDR-mediated genome editing efficiencies

Programmable double-strand DNA breaks (DSBs) can be harnessed for precision genome editing through manipulation of the homology-directed repair (HDR) pathway. However, end-joining repair pathways often outcompete HDR and introduce insertions and deletions of bases (indels) at the DSB site, decreasin...

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Detalles Bibliográficos
Autores principales:	Bodai, Zsolt, Bishop, Alena L., Gantz, Valentino M., Komor, Alexis C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085776/ https://www.ncbi.nlm.nih.gov/pubmed/35534455 http://dx.doi.org/10.1038/s41467-022-29989-9

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