Cargando…
The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization
The production of designer-length tobacco mosaic virus (TMV) nanorods in plants has been problematic in terms of yields, particularly when modified coat protein subunits are incorporated. To address this, we have investigated the use of a replicating potato virus X-based vector (pEff) to express def...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086362/ https://www.ncbi.nlm.nih.gov/pubmed/35557863 http://dx.doi.org/10.3389/fbioe.2022.877361 |
_version_ | 1784703982193606656 |
---|---|
author | Saunders, Keith Thuenemann, Eva C. Shah, Sachin N. Peyret, Hadrien Kristianingsih, Ruth Lopez, Sergio G. Richardson, Jake Lomonossoff, George P. |
author_facet | Saunders, Keith Thuenemann, Eva C. Shah, Sachin N. Peyret, Hadrien Kristianingsih, Ruth Lopez, Sergio G. Richardson, Jake Lomonossoff, George P. |
author_sort | Saunders, Keith |
collection | PubMed |
description | The production of designer-length tobacco mosaic virus (TMV) nanorods in plants has been problematic in terms of yields, particularly when modified coat protein subunits are incorporated. To address this, we have investigated the use of a replicating potato virus X-based vector (pEff) to express defined length nanorods containing either wild-type or modified versions of the TMV coat protein. This system has previously been shown to be an efficient method for producing virus-like particles of filamentous plant viruses. The length of the resulting TMV nanorods can be controlled by varying the length of the encapsidated RNA. Nanorod lengths were analyzed with a custom-written Python computer script coupled with the Nanorod UI user interface script, thereby generating histograms of particle length. In addition, nanorod variants were produced by incorporating coat protein subunits presenting metal-binding peptides at their C-termini. We demonstrate the utility of this approach by generating nanorods that bind colloidal gold nanoparticles. |
format | Online Article Text |
id | pubmed-9086362 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90863622022-05-11 The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization Saunders, Keith Thuenemann, Eva C. Shah, Sachin N. Peyret, Hadrien Kristianingsih, Ruth Lopez, Sergio G. Richardson, Jake Lomonossoff, George P. Front Bioeng Biotechnol Bioengineering and Biotechnology The production of designer-length tobacco mosaic virus (TMV) nanorods in plants has been problematic in terms of yields, particularly when modified coat protein subunits are incorporated. To address this, we have investigated the use of a replicating potato virus X-based vector (pEff) to express defined length nanorods containing either wild-type or modified versions of the TMV coat protein. This system has previously been shown to be an efficient method for producing virus-like particles of filamentous plant viruses. The length of the resulting TMV nanorods can be controlled by varying the length of the encapsidated RNA. Nanorod lengths were analyzed with a custom-written Python computer script coupled with the Nanorod UI user interface script, thereby generating histograms of particle length. In addition, nanorod variants were produced by incorporating coat protein subunits presenting metal-binding peptides at their C-termini. We demonstrate the utility of this approach by generating nanorods that bind colloidal gold nanoparticles. Frontiers Media S.A. 2022-04-26 /pmc/articles/PMC9086362/ /pubmed/35557863 http://dx.doi.org/10.3389/fbioe.2022.877361 Text en Copyright © 2022 Saunders, Thuenemann, Shah, Peyret, Kristianingsih, Lopez, Richardson and Lomonossoff. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Saunders, Keith Thuenemann, Eva C. Shah, Sachin N. Peyret, Hadrien Kristianingsih, Ruth Lopez, Sergio G. Richardson, Jake Lomonossoff, George P. The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization |
title | The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization |
title_full | The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization |
title_fullStr | The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization |
title_full_unstemmed | The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization |
title_short | The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization |
title_sort | use of a replicating virus vector for in planta generation of tobacco mosaic virus nanorods suitable for metallization |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086362/ https://www.ncbi.nlm.nih.gov/pubmed/35557863 http://dx.doi.org/10.3389/fbioe.2022.877361 |
work_keys_str_mv | AT saunderskeith theuseofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT thuenemannevac theuseofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT shahsachinn theuseofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT peyrethadrien theuseofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT kristianingsihruth theuseofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT lopezsergiog theuseofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT richardsonjake theuseofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT lomonossoffgeorgep theuseofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT saunderskeith useofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT thuenemannevac useofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT shahsachinn useofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT peyrethadrien useofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT kristianingsihruth useofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT lopezsergiog useofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT richardsonjake useofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization AT lomonossoffgeorgep useofareplicatingvirusvectorforinplantagenerationoftobaccomosaicvirusnanorodssuitableformetallization |