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The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization

The production of designer-length tobacco mosaic virus (TMV) nanorods in plants has been problematic in terms of yields, particularly when modified coat protein subunits are incorporated. To address this, we have investigated the use of a replicating potato virus X-based vector (pEff) to express def...

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Autores principales: Saunders, Keith, Thuenemann, Eva C., Shah, Sachin N., Peyret, Hadrien, Kristianingsih, Ruth, Lopez, Sergio G., Richardson, Jake, Lomonossoff, George P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086362/
https://www.ncbi.nlm.nih.gov/pubmed/35557863
http://dx.doi.org/10.3389/fbioe.2022.877361
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author Saunders, Keith
Thuenemann, Eva C.
Shah, Sachin N.
Peyret, Hadrien
Kristianingsih, Ruth
Lopez, Sergio G.
Richardson, Jake
Lomonossoff, George P.
author_facet Saunders, Keith
Thuenemann, Eva C.
Shah, Sachin N.
Peyret, Hadrien
Kristianingsih, Ruth
Lopez, Sergio G.
Richardson, Jake
Lomonossoff, George P.
author_sort Saunders, Keith
collection PubMed
description The production of designer-length tobacco mosaic virus (TMV) nanorods in plants has been problematic in terms of yields, particularly when modified coat protein subunits are incorporated. To address this, we have investigated the use of a replicating potato virus X-based vector (pEff) to express defined length nanorods containing either wild-type or modified versions of the TMV coat protein. This system has previously been shown to be an efficient method for producing virus-like particles of filamentous plant viruses. The length of the resulting TMV nanorods can be controlled by varying the length of the encapsidated RNA. Nanorod lengths were analyzed with a custom-written Python computer script coupled with the Nanorod UI user interface script, thereby generating histograms of particle length. In addition, nanorod variants were produced by incorporating coat protein subunits presenting metal-binding peptides at their C-termini. We demonstrate the utility of this approach by generating nanorods that bind colloidal gold nanoparticles.
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spelling pubmed-90863622022-05-11 The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization Saunders, Keith Thuenemann, Eva C. Shah, Sachin N. Peyret, Hadrien Kristianingsih, Ruth Lopez, Sergio G. Richardson, Jake Lomonossoff, George P. Front Bioeng Biotechnol Bioengineering and Biotechnology The production of designer-length tobacco mosaic virus (TMV) nanorods in plants has been problematic in terms of yields, particularly when modified coat protein subunits are incorporated. To address this, we have investigated the use of a replicating potato virus X-based vector (pEff) to express defined length nanorods containing either wild-type or modified versions of the TMV coat protein. This system has previously been shown to be an efficient method for producing virus-like particles of filamentous plant viruses. The length of the resulting TMV nanorods can be controlled by varying the length of the encapsidated RNA. Nanorod lengths were analyzed with a custom-written Python computer script coupled with the Nanorod UI user interface script, thereby generating histograms of particle length. In addition, nanorod variants were produced by incorporating coat protein subunits presenting metal-binding peptides at their C-termini. We demonstrate the utility of this approach by generating nanorods that bind colloidal gold nanoparticles. Frontiers Media S.A. 2022-04-26 /pmc/articles/PMC9086362/ /pubmed/35557863 http://dx.doi.org/10.3389/fbioe.2022.877361 Text en Copyright © 2022 Saunders, Thuenemann, Shah, Peyret, Kristianingsih, Lopez, Richardson and Lomonossoff. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Saunders, Keith
Thuenemann, Eva C.
Shah, Sachin N.
Peyret, Hadrien
Kristianingsih, Ruth
Lopez, Sergio G.
Richardson, Jake
Lomonossoff, George P.
The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization
title The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization
title_full The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization
title_fullStr The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization
title_full_unstemmed The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization
title_short The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization
title_sort use of a replicating virus vector for in planta generation of tobacco mosaic virus nanorods suitable for metallization
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086362/
https://www.ncbi.nlm.nih.gov/pubmed/35557863
http://dx.doi.org/10.3389/fbioe.2022.877361
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