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The Evidence of SARS-CoV-2 Human-to-Pets Transmission in Household Settings in Bosnia and Herzegovina

The infection with SARS-CoV-2 virus in cats and dogs raised issue of human-to-animal transmission of SARS-CoV-2 in domestic pets in close contacts with their owners. Our study was designed to research this in the framework of Bosnia and Herzegovina. Using ELISA, AFIAS fluorescent immunoassay, RT-qPC...

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Autores principales: Goletic, Sejla, Goletic, Teufik, Softic, Adis, Zahirovic, Amir, Rukavina, Dunja, Kavazovic, Aida, Omeragic, Jasmin, Umihanic, Sekib, Hukic, Mirsada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086552/
https://www.ncbi.nlm.nih.gov/pubmed/35559041
http://dx.doi.org/10.3389/fgene.2022.839205
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author Goletic, Sejla
Goletic, Teufik
Softic, Adis
Zahirovic, Amir
Rukavina, Dunja
Kavazovic, Aida
Omeragic, Jasmin
Umihanic, Sekib
Hukic, Mirsada
author_facet Goletic, Sejla
Goletic, Teufik
Softic, Adis
Zahirovic, Amir
Rukavina, Dunja
Kavazovic, Aida
Omeragic, Jasmin
Umihanic, Sekib
Hukic, Mirsada
author_sort Goletic, Sejla
collection PubMed
description The infection with SARS-CoV-2 virus in cats and dogs raised issue of human-to-animal transmission of SARS-CoV-2 in domestic pets in close contacts with their owners. Our study was designed to research this in the framework of Bosnia and Herzegovina. Using ELISA, AFIAS fluorescent immunoassay, RT-qPCR and WGS on Nanopore MinION platform with ARTIC Network Amplicon sequencing protocol for SARS-CoV-2, we showed that three out of thirteen dogs and one out of five cats from the households with confirmed human cases of COVID-19 in Bosnia-Herzegovina were infected with SARS-CoV-2. The high viral RNA load was detected in samples collected from a 4-year-old male Havanese (Ct = 12.52), a 6-year-old German Shepherd (Ct = 21.36) and a 9-year-old female American Staffordshire terrier (Ct = 25.74). The antibody response in dogs and one cat was observed. The viral genetic sequences from dogs were identical to the sequences detected in the owners suggesting the human-to-animal transmission of the virus. These findings, especially the low initial Ct values detected, from the public health perspective additionally stress the need for precautionary measures to protect both humans and animals.
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spelling pubmed-90865522022-05-11 The Evidence of SARS-CoV-2 Human-to-Pets Transmission in Household Settings in Bosnia and Herzegovina Goletic, Sejla Goletic, Teufik Softic, Adis Zahirovic, Amir Rukavina, Dunja Kavazovic, Aida Omeragic, Jasmin Umihanic, Sekib Hukic, Mirsada Front Genet Genetics The infection with SARS-CoV-2 virus in cats and dogs raised issue of human-to-animal transmission of SARS-CoV-2 in domestic pets in close contacts with their owners. Our study was designed to research this in the framework of Bosnia and Herzegovina. Using ELISA, AFIAS fluorescent immunoassay, RT-qPCR and WGS on Nanopore MinION platform with ARTIC Network Amplicon sequencing protocol for SARS-CoV-2, we showed that three out of thirteen dogs and one out of five cats from the households with confirmed human cases of COVID-19 in Bosnia-Herzegovina were infected with SARS-CoV-2. The high viral RNA load was detected in samples collected from a 4-year-old male Havanese (Ct = 12.52), a 6-year-old German Shepherd (Ct = 21.36) and a 9-year-old female American Staffordshire terrier (Ct = 25.74). The antibody response in dogs and one cat was observed. The viral genetic sequences from dogs were identical to the sequences detected in the owners suggesting the human-to-animal transmission of the virus. These findings, especially the low initial Ct values detected, from the public health perspective additionally stress the need for precautionary measures to protect both humans and animals. Frontiers Media S.A. 2022-04-26 /pmc/articles/PMC9086552/ /pubmed/35559041 http://dx.doi.org/10.3389/fgene.2022.839205 Text en Copyright © 2022 Goletic, Goletic, Softic, Zahirovic, Rukavina, Kavazovic, Omeragic, Umihanic and Hukic. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Goletic, Sejla
Goletic, Teufik
Softic, Adis
Zahirovic, Amir
Rukavina, Dunja
Kavazovic, Aida
Omeragic, Jasmin
Umihanic, Sekib
Hukic, Mirsada
The Evidence of SARS-CoV-2 Human-to-Pets Transmission in Household Settings in Bosnia and Herzegovina
title The Evidence of SARS-CoV-2 Human-to-Pets Transmission in Household Settings in Bosnia and Herzegovina
title_full The Evidence of SARS-CoV-2 Human-to-Pets Transmission in Household Settings in Bosnia and Herzegovina
title_fullStr The Evidence of SARS-CoV-2 Human-to-Pets Transmission in Household Settings in Bosnia and Herzegovina
title_full_unstemmed The Evidence of SARS-CoV-2 Human-to-Pets Transmission in Household Settings in Bosnia and Herzegovina
title_short The Evidence of SARS-CoV-2 Human-to-Pets Transmission in Household Settings in Bosnia and Herzegovina
title_sort evidence of sars-cov-2 human-to-pets transmission in household settings in bosnia and herzegovina
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086552/
https://www.ncbi.nlm.nih.gov/pubmed/35559041
http://dx.doi.org/10.3389/fgene.2022.839205
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