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Nanocharacterization of liposomes for the encapsulation of water soluble compounds from Cordyceps sinensis CS1197 by a supercritical gas anti-solvent technique

Nano-liposomes were designed for the sustained release of water soluble compounds from C. sinensis CS1197 using a supercritical gas anti-solvent (SC-GAS) method at various pressures, temperatures and Tween 80 concentrations. The SC-GAS method was compared to the Bangham method of liposome production...

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Detalles Bibliográficos
Autores principales: Shashidhar, G. M., Manohar, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086942/
https://www.ncbi.nlm.nih.gov/pubmed/35548621
http://dx.doi.org/10.1039/c8ra07601d
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author Shashidhar, G. M.
Manohar, B.
author_facet Shashidhar, G. M.
Manohar, B.
author_sort Shashidhar, G. M.
collection PubMed
description Nano-liposomes were designed for the sustained release of water soluble compounds from C. sinensis CS1197 using a supercritical gas anti-solvent (SC-GAS) method at various pressures, temperatures and Tween 80 concentrations. The SC-GAS method was compared to the Bangham method of liposome production in terms of mean diameter, coefficient of uniformity (C(u)), encapsulation efficiency, morphology, viscosity and actual energy required for liposome formation. Liposome production via the SC-GAS method under optimized formulation conditions, i.e., 180 bar; 50 °C; 0.75% Tween 80; and a depressurization rate of 25 bar min(−1), yielded nano-liposomes exhibiting the lowest C(u) value (1.10 ± 0.012) with a mean diameter of 0.072 ± 0.002 μm and better encapsulation efficiencies of 75.48 ± 2.5, 74.9 ± 2.1 and 70.23 ± 2.9% for adenosine, cordycepin and polysaccharides, respectively. Nano-liposomes were characterized using FTIR, XRD, DSC and TGA techniques. The stability indices and viscosities of the prepared liposome suspensions indicated good stability of up to 2 months and near-Newtonian behavior. The in vitro release of CS1197 water soluble compounds exhibited biphasic and sustained release patterns.
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spelling pubmed-90869422022-05-10 Nanocharacterization of liposomes for the encapsulation of water soluble compounds from Cordyceps sinensis CS1197 by a supercritical gas anti-solvent technique Shashidhar, G. M. Manohar, B. RSC Adv Chemistry Nano-liposomes were designed for the sustained release of water soluble compounds from C. sinensis CS1197 using a supercritical gas anti-solvent (SC-GAS) method at various pressures, temperatures and Tween 80 concentrations. The SC-GAS method was compared to the Bangham method of liposome production in terms of mean diameter, coefficient of uniformity (C(u)), encapsulation efficiency, morphology, viscosity and actual energy required for liposome formation. Liposome production via the SC-GAS method under optimized formulation conditions, i.e., 180 bar; 50 °C; 0.75% Tween 80; and a depressurization rate of 25 bar min(−1), yielded nano-liposomes exhibiting the lowest C(u) value (1.10 ± 0.012) with a mean diameter of 0.072 ± 0.002 μm and better encapsulation efficiencies of 75.48 ± 2.5, 74.9 ± 2.1 and 70.23 ± 2.9% for adenosine, cordycepin and polysaccharides, respectively. Nano-liposomes were characterized using FTIR, XRD, DSC and TGA techniques. The stability indices and viscosities of the prepared liposome suspensions indicated good stability of up to 2 months and near-Newtonian behavior. The in vitro release of CS1197 water soluble compounds exhibited biphasic and sustained release patterns. The Royal Society of Chemistry 2018-10-09 /pmc/articles/PMC9086942/ /pubmed/35548621 http://dx.doi.org/10.1039/c8ra07601d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Shashidhar, G. M.
Manohar, B.
Nanocharacterization of liposomes for the encapsulation of water soluble compounds from Cordyceps sinensis CS1197 by a supercritical gas anti-solvent technique
title Nanocharacterization of liposomes for the encapsulation of water soluble compounds from Cordyceps sinensis CS1197 by a supercritical gas anti-solvent technique
title_full Nanocharacterization of liposomes for the encapsulation of water soluble compounds from Cordyceps sinensis CS1197 by a supercritical gas anti-solvent technique
title_fullStr Nanocharacterization of liposomes for the encapsulation of water soluble compounds from Cordyceps sinensis CS1197 by a supercritical gas anti-solvent technique
title_full_unstemmed Nanocharacterization of liposomes for the encapsulation of water soluble compounds from Cordyceps sinensis CS1197 by a supercritical gas anti-solvent technique
title_short Nanocharacterization of liposomes for the encapsulation of water soluble compounds from Cordyceps sinensis CS1197 by a supercritical gas anti-solvent technique
title_sort nanocharacterization of liposomes for the encapsulation of water soluble compounds from cordyceps sinensis cs1197 by a supercritical gas anti-solvent technique
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086942/
https://www.ncbi.nlm.nih.gov/pubmed/35548621
http://dx.doi.org/10.1039/c8ra07601d
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