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Polydopamine-based nanoparticles with excellent biocompatibility for photothermally enhanced gene delivery
For non-viral gene delivery systems, desirable endosomal release is crucial for the achievement of optimum therapeutic efficacy. In this work, polyethylenimine-modified polydopamine-based nanoparticles (PPNPs) with excellent biocompatibility were prepared. These PPNPs showed an average diameter of 1...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086945/ https://www.ncbi.nlm.nih.gov/pubmed/35548626 http://dx.doi.org/10.1039/c8ra06916f |
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author | Zhang, Peng Xu, Qinan Du, Jianwei Wang, Youxiang |
author_facet | Zhang, Peng Xu, Qinan Du, Jianwei Wang, Youxiang |
author_sort | Zhang, Peng |
collection | PubMed |
description | For non-viral gene delivery systems, desirable endosomal release is crucial for the achievement of optimum therapeutic efficacy. In this work, polyethylenimine-modified polydopamine-based nanoparticles (PPNPs) with excellent biocompatibility were prepared. These PPNPs showed an average diameter of 13 nm with narrow size distribution. Besides, they could load pGL3 DNA effectively at a mass ratio of PPNPs to DNA above 5 and form complexes with spherical morphology (60–80 nm). And PPNPs/DNA complexes demonstrated good photothermal conversion ability. Due to the excellent biocompatibility of polydopamine, these PPNPs/DNA complexes showed low cytotoxicity to HepG2 cells, even after 15 minutes of NIR light irradiation. Furthermore, the PPNPs/DNA complexes with mass ratios of 23 and 30 showed higher transfection levels than Lipofectamine 2000. After exposing these complexes to near infrared (NIR) light with a power density of 2.6 W cm(−2) for 15 min, the transfection level of PPNPs/DNA complexes tripled in HepG2 cells. The rise in gene transfection was attributed to the locally induced heat produced by the PPNPs/DNA complexes, which promoted endosomal membrane disruption and led to better endosomal escape. This result was also confirmed by confocal laser scanning microscope observation. Moreover, PPNPs/DNA complexes demonstrated excellent biocompatibility in hemolysis assays. At the mass ratio of 23 and DNA concentration of 20 μg mL(−1), the hemolysis ratio of the PPNPs/DNA complexes was only 1%, lower than that of the PEI/DNA complexes. This PPNP nanocarrier was inspiring for the design of non-viral gene delivery systems with promoted therapeutic efficacy. |
format | Online Article Text |
id | pubmed-9086945 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90869452022-05-10 Polydopamine-based nanoparticles with excellent biocompatibility for photothermally enhanced gene delivery Zhang, Peng Xu, Qinan Du, Jianwei Wang, Youxiang RSC Adv Chemistry For non-viral gene delivery systems, desirable endosomal release is crucial for the achievement of optimum therapeutic efficacy. In this work, polyethylenimine-modified polydopamine-based nanoparticles (PPNPs) with excellent biocompatibility were prepared. These PPNPs showed an average diameter of 13 nm with narrow size distribution. Besides, they could load pGL3 DNA effectively at a mass ratio of PPNPs to DNA above 5 and form complexes with spherical morphology (60–80 nm). And PPNPs/DNA complexes demonstrated good photothermal conversion ability. Due to the excellent biocompatibility of polydopamine, these PPNPs/DNA complexes showed low cytotoxicity to HepG2 cells, even after 15 minutes of NIR light irradiation. Furthermore, the PPNPs/DNA complexes with mass ratios of 23 and 30 showed higher transfection levels than Lipofectamine 2000. After exposing these complexes to near infrared (NIR) light with a power density of 2.6 W cm(−2) for 15 min, the transfection level of PPNPs/DNA complexes tripled in HepG2 cells. The rise in gene transfection was attributed to the locally induced heat produced by the PPNPs/DNA complexes, which promoted endosomal membrane disruption and led to better endosomal escape. This result was also confirmed by confocal laser scanning microscope observation. Moreover, PPNPs/DNA complexes demonstrated excellent biocompatibility in hemolysis assays. At the mass ratio of 23 and DNA concentration of 20 μg mL(−1), the hemolysis ratio of the PPNPs/DNA complexes was only 1%, lower than that of the PEI/DNA complexes. This PPNP nanocarrier was inspiring for the design of non-viral gene delivery systems with promoted therapeutic efficacy. The Royal Society of Chemistry 2018-10-09 /pmc/articles/PMC9086945/ /pubmed/35548626 http://dx.doi.org/10.1039/c8ra06916f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Zhang, Peng Xu, Qinan Du, Jianwei Wang, Youxiang Polydopamine-based nanoparticles with excellent biocompatibility for photothermally enhanced gene delivery |
title | Polydopamine-based nanoparticles with excellent biocompatibility for photothermally enhanced gene delivery |
title_full | Polydopamine-based nanoparticles with excellent biocompatibility for photothermally enhanced gene delivery |
title_fullStr | Polydopamine-based nanoparticles with excellent biocompatibility for photothermally enhanced gene delivery |
title_full_unstemmed | Polydopamine-based nanoparticles with excellent biocompatibility for photothermally enhanced gene delivery |
title_short | Polydopamine-based nanoparticles with excellent biocompatibility for photothermally enhanced gene delivery |
title_sort | polydopamine-based nanoparticles with excellent biocompatibility for photothermally enhanced gene delivery |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086945/ https://www.ncbi.nlm.nih.gov/pubmed/35548626 http://dx.doi.org/10.1039/c8ra06916f |
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