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Simultaneous analysis of antigen‐specific B and T cells after SARS‐CoV‐2 infection and vaccination

Conventional methods for quantifying and phenotyping antigen‐specific lymphocytes can rapidly deplete irreplaceable specimens. This is due to the fact that antigen‐specific T and B cells have historically been analyzed in independent assays each requiring millions of cells. A technique that facilita...

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Detalles Bibliográficos
Autores principales: Newell, Krista L., Waldran, Mitchell J., Thomas, Stephen J., Endy, Timothy P., Waickman, Adam T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9087145/
https://www.ncbi.nlm.nih.gov/pubmed/35468250
http://dx.doi.org/10.1002/cyto.a.24563
Descripción
Sumario:Conventional methods for quantifying and phenotyping antigen‐specific lymphocytes can rapidly deplete irreplaceable specimens. This is due to the fact that antigen‐specific T and B cells have historically been analyzed in independent assays each requiring millions of cells. A technique that facilitates the simultaneous detection of antigen‐specific T and B cells would allow for more thorough immune profiling with significantly reduced sample requirements. To this end, we developed the B and T cell tandem lymphocyte evaluation (BATTLE) assay, which allows for the simultaneous identification of SARS‐CoV‐2 Spike reactive T and B cells using an activation induced marker (AIM) T cell assay and dual‐color B cell antigen probes. Using this assay, we demonstrate that antigen‐specific B and T cell subsets can be identified simultaneously using conventional flow cytometry platforms and provide insight into the differential effects of mRNA vaccination on B and T cell populations following natural SARS‐CoV‐2 infection.