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Simultaneous analysis of antigen‐specific B and T cells after SARS‐CoV‐2 infection and vaccination
Conventional methods for quantifying and phenotyping antigen‐specific lymphocytes can rapidly deplete irreplaceable specimens. This is due to the fact that antigen‐specific T and B cells have historically been analyzed in independent assays each requiring millions of cells. A technique that facilita...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9087145/ https://www.ncbi.nlm.nih.gov/pubmed/35468250 http://dx.doi.org/10.1002/cyto.a.24563 |
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author | Newell, Krista L. Waldran, Mitchell J. Thomas, Stephen J. Endy, Timothy P. Waickman, Adam T. |
author_facet | Newell, Krista L. Waldran, Mitchell J. Thomas, Stephen J. Endy, Timothy P. Waickman, Adam T. |
author_sort | Newell, Krista L. |
collection | PubMed |
description | Conventional methods for quantifying and phenotyping antigen‐specific lymphocytes can rapidly deplete irreplaceable specimens. This is due to the fact that antigen‐specific T and B cells have historically been analyzed in independent assays each requiring millions of cells. A technique that facilitates the simultaneous detection of antigen‐specific T and B cells would allow for more thorough immune profiling with significantly reduced sample requirements. To this end, we developed the B and T cell tandem lymphocyte evaluation (BATTLE) assay, which allows for the simultaneous identification of SARS‐CoV‐2 Spike reactive T and B cells using an activation induced marker (AIM) T cell assay and dual‐color B cell antigen probes. Using this assay, we demonstrate that antigen‐specific B and T cell subsets can be identified simultaneously using conventional flow cytometry platforms and provide insight into the differential effects of mRNA vaccination on B and T cell populations following natural SARS‐CoV‐2 infection. |
format | Online Article Text |
id | pubmed-9087145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90871452022-05-10 Simultaneous analysis of antigen‐specific B and T cells after SARS‐CoV‐2 infection and vaccination Newell, Krista L. Waldran, Mitchell J. Thomas, Stephen J. Endy, Timothy P. Waickman, Adam T. Cytometry A Covid‐19 Conventional methods for quantifying and phenotyping antigen‐specific lymphocytes can rapidly deplete irreplaceable specimens. This is due to the fact that antigen‐specific T and B cells have historically been analyzed in independent assays each requiring millions of cells. A technique that facilitates the simultaneous detection of antigen‐specific T and B cells would allow for more thorough immune profiling with significantly reduced sample requirements. To this end, we developed the B and T cell tandem lymphocyte evaluation (BATTLE) assay, which allows for the simultaneous identification of SARS‐CoV‐2 Spike reactive T and B cells using an activation induced marker (AIM) T cell assay and dual‐color B cell antigen probes. Using this assay, we demonstrate that antigen‐specific B and T cell subsets can be identified simultaneously using conventional flow cytometry platforms and provide insight into the differential effects of mRNA vaccination on B and T cell populations following natural SARS‐CoV‐2 infection. John Wiley & Sons, Inc. 2022-05-05 2022-06 /pmc/articles/PMC9087145/ /pubmed/35468250 http://dx.doi.org/10.1002/cyto.a.24563 Text en © 2022 The Authors. Cytometry Part A published by Wiley Periodicals LLC on behalf of International Society for Advancement of Cytometry. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Covid‐19 Newell, Krista L. Waldran, Mitchell J. Thomas, Stephen J. Endy, Timothy P. Waickman, Adam T. Simultaneous analysis of antigen‐specific B and T cells after SARS‐CoV‐2 infection and vaccination |
title | Simultaneous analysis of antigen‐specific B and T cells after SARS‐CoV‐2 infection and vaccination |
title_full | Simultaneous analysis of antigen‐specific B and T cells after SARS‐CoV‐2 infection and vaccination |
title_fullStr | Simultaneous analysis of antigen‐specific B and T cells after SARS‐CoV‐2 infection and vaccination |
title_full_unstemmed | Simultaneous analysis of antigen‐specific B and T cells after SARS‐CoV‐2 infection and vaccination |
title_short | Simultaneous analysis of antigen‐specific B and T cells after SARS‐CoV‐2 infection and vaccination |
title_sort | simultaneous analysis of antigen‐specific b and t cells after sars‐cov‐2 infection and vaccination |
topic | Covid‐19 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9087145/ https://www.ncbi.nlm.nih.gov/pubmed/35468250 http://dx.doi.org/10.1002/cyto.a.24563 |
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