Cargando…

Highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells

As one of the carbon based materials, the potential application of carbon nanoparticles (CNPs) has emerged in the promotion of plant growth. However, knowledge on the biological mechanism of how the CNPs interact with plant cells is limited. In this study, nanostructures of CNPs were examined. The p...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Lijuan, Wang, Hongbo, Li, Xiang, Nie, Cong, Liang, Taibo, Xie, Fuwei, Liu, Kejian, Peng, Xiaojun, Xie, Jianping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9087372/
https://www.ncbi.nlm.nih.gov/pubmed/35547047
http://dx.doi.org/10.1039/c8ra06665e
_version_ 1784704189982572544
author Chen, Lijuan
Wang, Hongbo
Li, Xiang
Nie, Cong
Liang, Taibo
Xie, Fuwei
Liu, Kejian
Peng, Xiaojun
Xie, Jianping
author_facet Chen, Lijuan
Wang, Hongbo
Li, Xiang
Nie, Cong
Liang, Taibo
Xie, Fuwei
Liu, Kejian
Peng, Xiaojun
Xie, Jianping
author_sort Chen, Lijuan
collection PubMed
description As one of the carbon based materials, the potential application of carbon nanoparticles (CNPs) has emerged in the promotion of plant growth. However, knowledge on the biological mechanism of how the CNPs interact with plant cells is limited. In this study, nanostructures of CNPs were examined. The particles exhibited particulate morphology and their size distribution was in the range of 18 to 70 nm, with an average size of 30 nm. Hydrophilic groups of COOH and OH were present on the surface of CNPs, and CNPs showed the common feature of graphitic sp(2) hybridization carbons. The CNPs were determined to be biocompatible with these two cell lines, mammalian cells (A549 cells) and plant cells (BY-2 cells). The COOH groups on the surface of CNPs were functionalized via covalent binding with a fluorescent dye for improvement of the fluorescence. The fluorescent carbon nanoparticles (FCNPs) were found to cross the cell membrane and enter cells (A549 cells and BY-2 cells) in an energy-dependent manner. Subsequently, the mechanism of FCNPs interaction with the cell membrane was evaluated in the presence of inhibitors that specifically affect different endocytosis membrane proteins. The FCNPs mainly entered A549 cells through caveolin-mediated endocytosis and macropinocytosis, and clathrin-dependent endocytosis was also involved in the transportation of the FCNPs. Clathrin-independent endocytosis mediated in the internalization of FCNPs in BY-2 cells. The way FCNPs entering cells will provide a fundamental understanding of the influence of CNPs on cell membrane.
format Online
Article
Text
id pubmed-9087372
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90873722022-05-10 Highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells Chen, Lijuan Wang, Hongbo Li, Xiang Nie, Cong Liang, Taibo Xie, Fuwei Liu, Kejian Peng, Xiaojun Xie, Jianping RSC Adv Chemistry As one of the carbon based materials, the potential application of carbon nanoparticles (CNPs) has emerged in the promotion of plant growth. However, knowledge on the biological mechanism of how the CNPs interact with plant cells is limited. In this study, nanostructures of CNPs were examined. The particles exhibited particulate morphology and their size distribution was in the range of 18 to 70 nm, with an average size of 30 nm. Hydrophilic groups of COOH and OH were present on the surface of CNPs, and CNPs showed the common feature of graphitic sp(2) hybridization carbons. The CNPs were determined to be biocompatible with these two cell lines, mammalian cells (A549 cells) and plant cells (BY-2 cells). The COOH groups on the surface of CNPs were functionalized via covalent binding with a fluorescent dye for improvement of the fluorescence. The fluorescent carbon nanoparticles (FCNPs) were found to cross the cell membrane and enter cells (A549 cells and BY-2 cells) in an energy-dependent manner. Subsequently, the mechanism of FCNPs interaction with the cell membrane was evaluated in the presence of inhibitors that specifically affect different endocytosis membrane proteins. The FCNPs mainly entered A549 cells through caveolin-mediated endocytosis and macropinocytosis, and clathrin-dependent endocytosis was also involved in the transportation of the FCNPs. Clathrin-independent endocytosis mediated in the internalization of FCNPs in BY-2 cells. The way FCNPs entering cells will provide a fundamental understanding of the influence of CNPs on cell membrane. The Royal Society of Chemistry 2018-10-15 /pmc/articles/PMC9087372/ /pubmed/35547047 http://dx.doi.org/10.1039/c8ra06665e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Chen, Lijuan
Wang, Hongbo
Li, Xiang
Nie, Cong
Liang, Taibo
Xie, Fuwei
Liu, Kejian
Peng, Xiaojun
Xie, Jianping
Highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells
title Highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells
title_full Highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells
title_fullStr Highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells
title_full_unstemmed Highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells
title_short Highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells
title_sort highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9087372/
https://www.ncbi.nlm.nih.gov/pubmed/35547047
http://dx.doi.org/10.1039/c8ra06665e
work_keys_str_mv AT chenlijuan highlyhydrophiliccarbonnanoparticlesuptakemechanismbymammalianandplantcells
AT wanghongbo highlyhydrophiliccarbonnanoparticlesuptakemechanismbymammalianandplantcells
AT lixiang highlyhydrophiliccarbonnanoparticlesuptakemechanismbymammalianandplantcells
AT niecong highlyhydrophiliccarbonnanoparticlesuptakemechanismbymammalianandplantcells
AT liangtaibo highlyhydrophiliccarbonnanoparticlesuptakemechanismbymammalianandplantcells
AT xiefuwei highlyhydrophiliccarbonnanoparticlesuptakemechanismbymammalianandplantcells
AT liukejian highlyhydrophiliccarbonnanoparticlesuptakemechanismbymammalianandplantcells
AT pengxiaojun highlyhydrophiliccarbonnanoparticlesuptakemechanismbymammalianandplantcells
AT xiejianping highlyhydrophiliccarbonnanoparticlesuptakemechanismbymammalianandplantcells