A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation

A rapid biosensor for the detection of cyromazine in milk is reported based on a fluorescence quenching result. When an FAM labelled G-rich ssDNA Tcy2 is treated with cyromazine, it can form a G-quadruplex-CYR complex and cause a change in fluorescence. As a result, the presence of cyromazine can be...

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Detalles Bibliográficos
Autores principales: Xing, Haibo, Gu, Wenchao, Xu, Dang, Tian, Fuxiang, Yao, Linyun, Wang, Zhenwei, Hu, Xiaojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9088062/
https://www.ncbi.nlm.nih.gov/pubmed/35546964
http://dx.doi.org/10.1039/c7ra12970j
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author Xing, Haibo
Gu, Wenchao
Xu, Dang
Tian, Fuxiang
Yao, Linyun
Wang, Zhenwei
Hu, Xiaojun
author_facet Xing, Haibo
Gu, Wenchao
Xu, Dang
Tian, Fuxiang
Yao, Linyun
Wang, Zhenwei
Hu, Xiaojun
author_sort Xing, Haibo
collection PubMed
description A rapid biosensor for the detection of cyromazine in milk is reported based on a fluorescence quenching result. When an FAM labelled G-rich ssDNA Tcy2 is treated with cyromazine, it can form a G-quadruplex-CYR complex and cause a change in fluorescence. As a result, the presence of cyromazine can be determined by fluorescence quenching. This sensor is selective for the detection of cyromazine in raw milk and has a limit of detection of 0.68 ppb and a detection range from 0 to 200 ppb.
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spelling pubmed-90880622022-05-10 A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation Xing, Haibo Gu, Wenchao Xu, Dang Tian, Fuxiang Yao, Linyun Wang, Zhenwei Hu, Xiaojun RSC Adv Chemistry A rapid biosensor for the detection of cyromazine in milk is reported based on a fluorescence quenching result. When an FAM labelled G-rich ssDNA Tcy2 is treated with cyromazine, it can form a G-quadruplex-CYR complex and cause a change in fluorescence. As a result, the presence of cyromazine can be determined by fluorescence quenching. This sensor is selective for the detection of cyromazine in raw milk and has a limit of detection of 0.68 ppb and a detection range from 0 to 200 ppb. The Royal Society of Chemistry 2018-01-10 /pmc/articles/PMC9088062/ /pubmed/35546964 http://dx.doi.org/10.1039/c7ra12970j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Xing, Haibo
Gu, Wenchao
Xu, Dang
Tian, Fuxiang
Yao, Linyun
Wang, Zhenwei
Hu, Xiaojun
A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation
title A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation
title_full A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation
title_fullStr A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation
title_full_unstemmed A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation
title_short A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation
title_sort simple fluorescent assay for cyromazine detection in raw milk by using cyr-stabilized g-quadruplex formation
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9088062/
https://www.ncbi.nlm.nih.gov/pubmed/35546964
http://dx.doi.org/10.1039/c7ra12970j
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