Rapid and visual readout of vitamin B1 based on the electrostatic interaction induced aggregation of gold nanoparticles

In this work, a simple and rapid colorimetric assay for the quantitative detection of vitamin B1 (VB1) has been fabricated based on citrate-stabilized gold nanoparticles (AuNPs). The UV-Vis spectra of AuNPs varied and the relative color changed from red to purple with the sequential addition of VB1. The characterization results of AuNPs with and without the addition of VB1 confirmed that the observed phenomena were attributed to the aggregation of AuNPs induced by VB1 through electrostatic interaction. The assay was rapid and sensitive to VB1 with a detection limit of 10.9 nM ranging from 30 nM to 650 nM in 15 min. Meanwhile, the developed assay displayed excellent selectivity to VB1 since AuNPs showed negligible response to common metal ions and biological molecules. Moreover, the feasibility for the quantitative detection of VB1 in tablets and human urine samples has also been demonstrated.