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Sonodynamic therapy induces oxidative stress, DNA damage and apoptosis in glioma cells

Malignant glioma remains one of the most challenging diseases to treat because of the invasive growth of glioma cells and the existence of the blood–brain barrier (BBB), which blocks drug delivery to the brain. New strategies are urgently needed to overcome these shortcomings and improve the outcome...

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Autores principales: Sun, Yue, Wang, Haiping, Zhang, Kun, Liu, Jingfei, Wang, Pan, Wang, Xiaobing, Liu, Quanhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9088833/
https://www.ncbi.nlm.nih.gov/pubmed/35558463
http://dx.doi.org/10.1039/c8ra07099g
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author Sun, Yue
Wang, Haiping
Zhang, Kun
Liu, Jingfei
Wang, Pan
Wang, Xiaobing
Liu, Quanhong
author_facet Sun, Yue
Wang, Haiping
Zhang, Kun
Liu, Jingfei
Wang, Pan
Wang, Xiaobing
Liu, Quanhong
author_sort Sun, Yue
collection PubMed
description Malignant glioma remains one of the most challenging diseases to treat because of the invasive growth of glioma cells and the existence of the blood–brain barrier (BBB), which blocks drug delivery to the brain. New strategies are urgently needed to overcome these shortcomings and improve the outcomes. Ultrasound represents a promising noninvasive and reversible BBB opening approach and the related sonodynamic therapy (SDT) is rapidly emerging. This study aims to explore the ultrasound parameters for BBB opening and the cell killing effect of SDT in human glioma U373 cells by using a recently reported sonosensitizer, sinoporphyrin sodium (DVDMS). The in vitro BBB model indicated that SDT caused a time-dependent permeability increase, which peaked at 2 h post treatment and then recovered gradually. The results of toxicology tests showed significant U373 cell viability loss and apoptosis increase after DVDMS-SDT, accompanied by enhanced cleaved-caspase-3 level and DNA fragmentation, in which reactive oxygen species (ROS) were a major triggering intermediate during DVDMS-SDT. Furthermore, DVDMS-SDT produced DNA damage and the underlying mechanisms were evaluated, in order to provide a fundamental basis for DVDMS-SDT application in glioma therapy. The findings indicated that the DNA molecules could be temporarily regulated by SDT and DNA double-strand breaks (DSBs), which increased the difficulty of cellular self-repair, thus aggravating cell apoptosis and inhibiting glioma cell invasive growth. Therefore, this study supports the use of SDT as an alternative approach for glioma therapy.
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spelling pubmed-90888332022-05-11 Sonodynamic therapy induces oxidative stress, DNA damage and apoptosis in glioma cells Sun, Yue Wang, Haiping Zhang, Kun Liu, Jingfei Wang, Pan Wang, Xiaobing Liu, Quanhong RSC Adv Chemistry Malignant glioma remains one of the most challenging diseases to treat because of the invasive growth of glioma cells and the existence of the blood–brain barrier (BBB), which blocks drug delivery to the brain. New strategies are urgently needed to overcome these shortcomings and improve the outcomes. Ultrasound represents a promising noninvasive and reversible BBB opening approach and the related sonodynamic therapy (SDT) is rapidly emerging. This study aims to explore the ultrasound parameters for BBB opening and the cell killing effect of SDT in human glioma U373 cells by using a recently reported sonosensitizer, sinoporphyrin sodium (DVDMS). The in vitro BBB model indicated that SDT caused a time-dependent permeability increase, which peaked at 2 h post treatment and then recovered gradually. The results of toxicology tests showed significant U373 cell viability loss and apoptosis increase after DVDMS-SDT, accompanied by enhanced cleaved-caspase-3 level and DNA fragmentation, in which reactive oxygen species (ROS) were a major triggering intermediate during DVDMS-SDT. Furthermore, DVDMS-SDT produced DNA damage and the underlying mechanisms were evaluated, in order to provide a fundamental basis for DVDMS-SDT application in glioma therapy. The findings indicated that the DNA molecules could be temporarily regulated by SDT and DNA double-strand breaks (DSBs), which increased the difficulty of cellular self-repair, thus aggravating cell apoptosis and inhibiting glioma cell invasive growth. Therefore, this study supports the use of SDT as an alternative approach for glioma therapy. The Royal Society of Chemistry 2018-10-25 /pmc/articles/PMC9088833/ /pubmed/35558463 http://dx.doi.org/10.1039/c8ra07099g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Sun, Yue
Wang, Haiping
Zhang, Kun
Liu, Jingfei
Wang, Pan
Wang, Xiaobing
Liu, Quanhong
Sonodynamic therapy induces oxidative stress, DNA damage and apoptosis in glioma cells
title Sonodynamic therapy induces oxidative stress, DNA damage and apoptosis in glioma cells
title_full Sonodynamic therapy induces oxidative stress, DNA damage and apoptosis in glioma cells
title_fullStr Sonodynamic therapy induces oxidative stress, DNA damage and apoptosis in glioma cells
title_full_unstemmed Sonodynamic therapy induces oxidative stress, DNA damage and apoptosis in glioma cells
title_short Sonodynamic therapy induces oxidative stress, DNA damage and apoptosis in glioma cells
title_sort sonodynamic therapy induces oxidative stress, dna damage and apoptosis in glioma cells
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9088833/
https://www.ncbi.nlm.nih.gov/pubmed/35558463
http://dx.doi.org/10.1039/c8ra07099g
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