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Universally applicable, quantitative PCR method utilizing fluorescent nucleobase analogs

We herein describe a novel quantitative PCR (qPCR) method, which operates in both signal-off and on manners, by utilizing a unique property of fluorescent nucleobase analogs. The first, signal-off method is developed by designing the primers to contain pyrrolo-dC (PdC), one of the most common fluore...

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Detalles Bibliográficos
Autores principales:	Kim, Hyo Yong, Li, Taihua, Jung, Cheulhee, Fu, Rongzhan, Cho, Dae-Yeon, Park, Ki Soo, Park, Hyun Gyu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Royal Society of Chemistry 2018
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089284/ https://www.ncbi.nlm.nih.gov/pubmed/35557795 http://dx.doi.org/10.1039/c8ra06675b

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