Cargando…

Determination of major and trace element variability in healthy human urine by ICP-QMS and specific gravity normalisation

Sixty five urine samples obtained during one or two non-consecutive days from 10 healthy individuals were analysed for major (Na, Mg, K, Ca) and trace (Co, Cu, Zn, As, Rb, Sr, Mo and Pb) element concentrations. Following microwave digestion, the analyses were carried out using ICP-QMS (inductively c...

Descripción completa

Detalles Bibliográficos
Autores principales: E. T. Moore, Rebekah, Rehkämper, Mark, Kreissig, Katharina, Strekopytov, Stanislav, Larner, Fiona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089848/
https://www.ncbi.nlm.nih.gov/pubmed/35558613
http://dx.doi.org/10.1039/c8ra06794e
_version_ 1784704601747881984
author E. T. Moore, Rebekah
Rehkämper, Mark
Kreissig, Katharina
Strekopytov, Stanislav
Larner, Fiona
author_facet E. T. Moore, Rebekah
Rehkämper, Mark
Kreissig, Katharina
Strekopytov, Stanislav
Larner, Fiona
author_sort E. T. Moore, Rebekah
collection PubMed
description Sixty five urine samples obtained during one or two non-consecutive days from 10 healthy individuals were analysed for major (Na, Mg, K, Ca) and trace (Co, Cu, Zn, As, Rb, Sr, Mo and Pb) element concentrations. Following microwave digestion, the analyses were carried out using ICP-QMS (inductively coupled plasma quadrupole mass spectrometry) incorporating a collision/reaction cell. Repeat analyses of quality control samples show that the procedure produces unbiased results and is well suited for routine urinalysis of the investigated elements. Concentrations were normalised using specific gravity (SG) and the resultant decrease in variability supports previous conclusions that SG-normalisation appropriately corrects for differences in urine dilution. The elemental concentrations of the individual urine samples show large differences in dispersion. Most variable are As, Co and Zn, with CVs (coefficients of variation) of >75%. The major elements as well as Rb, Sr and Mo display intermediate variability, whilst Cu and Pb have the least elemental dispersion with CV values of about 30%. A detailed assessment shows that the overall elemental variability is governed both by differences between individuals and variations for a single individual over time. Spot urine samples exhibit elemental concentrations that, on average, resemble the daily mean values to within about 30% for all elements except K and Rb. Diet-related changes in urinary element concentration are most prominent for Mg, K, Co, Rb and Pb. The concentrations of Co, As and Rb appear to vary systematically with gender but this may primarily reflect co-variance with specific diets.
format Online
Article
Text
id pubmed-9089848
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90898482022-05-11 Determination of major and trace element variability in healthy human urine by ICP-QMS and specific gravity normalisation E. T. Moore, Rebekah Rehkämper, Mark Kreissig, Katharina Strekopytov, Stanislav Larner, Fiona RSC Adv Chemistry Sixty five urine samples obtained during one or two non-consecutive days from 10 healthy individuals were analysed for major (Na, Mg, K, Ca) and trace (Co, Cu, Zn, As, Rb, Sr, Mo and Pb) element concentrations. Following microwave digestion, the analyses were carried out using ICP-QMS (inductively coupled plasma quadrupole mass spectrometry) incorporating a collision/reaction cell. Repeat analyses of quality control samples show that the procedure produces unbiased results and is well suited for routine urinalysis of the investigated elements. Concentrations were normalised using specific gravity (SG) and the resultant decrease in variability supports previous conclusions that SG-normalisation appropriately corrects for differences in urine dilution. The elemental concentrations of the individual urine samples show large differences in dispersion. Most variable are As, Co and Zn, with CVs (coefficients of variation) of >75%. The major elements as well as Rb, Sr and Mo display intermediate variability, whilst Cu and Pb have the least elemental dispersion with CV values of about 30%. A detailed assessment shows that the overall elemental variability is governed both by differences between individuals and variations for a single individual over time. Spot urine samples exhibit elemental concentrations that, on average, resemble the daily mean values to within about 30% for all elements except K and Rb. Diet-related changes in urinary element concentration are most prominent for Mg, K, Co, Rb and Pb. The concentrations of Co, As and Rb appear to vary systematically with gender but this may primarily reflect co-variance with specific diets. The Royal Society of Chemistry 2018-11-13 /pmc/articles/PMC9089848/ /pubmed/35558613 http://dx.doi.org/10.1039/c8ra06794e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
E. T. Moore, Rebekah
Rehkämper, Mark
Kreissig, Katharina
Strekopytov, Stanislav
Larner, Fiona
Determination of major and trace element variability in healthy human urine by ICP-QMS and specific gravity normalisation
title Determination of major and trace element variability in healthy human urine by ICP-QMS and specific gravity normalisation
title_full Determination of major and trace element variability in healthy human urine by ICP-QMS and specific gravity normalisation
title_fullStr Determination of major and trace element variability in healthy human urine by ICP-QMS and specific gravity normalisation
title_full_unstemmed Determination of major and trace element variability in healthy human urine by ICP-QMS and specific gravity normalisation
title_short Determination of major and trace element variability in healthy human urine by ICP-QMS and specific gravity normalisation
title_sort determination of major and trace element variability in healthy human urine by icp-qms and specific gravity normalisation
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089848/
https://www.ncbi.nlm.nih.gov/pubmed/35558613
http://dx.doi.org/10.1039/c8ra06794e
work_keys_str_mv AT etmoorerebekah determinationofmajorandtraceelementvariabilityinhealthyhumanurinebyicpqmsandspecificgravitynormalisation
AT rehkampermark determinationofmajorandtraceelementvariabilityinhealthyhumanurinebyicpqmsandspecificgravitynormalisation
AT kreissigkatharina determinationofmajorandtraceelementvariabilityinhealthyhumanurinebyicpqmsandspecificgravitynormalisation
AT strekopytovstanislav determinationofmajorandtraceelementvariabilityinhealthyhumanurinebyicpqmsandspecificgravitynormalisation
AT larnerfiona determinationofmajorandtraceelementvariabilityinhealthyhumanurinebyicpqmsandspecificgravitynormalisation