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Environmental DNA detection of giant snakehead in Thailand’s major rivers for wild stock assessment

Capture-based aquaculture is now gaining much attention in Southeast Asia. This system was used to produce several fish species with social and economic implications, including the giant snakehead (Channa micropeltes). As wild harvesting of organisms for seed stock is one of main practices in captur...

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Detalles Bibliográficos
Autores principales: Osathanunkul, Maslin, Madesis, Panagiotis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089910/
https://www.ncbi.nlm.nih.gov/pubmed/35536840
http://dx.doi.org/10.1371/journal.pone.0267667
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author Osathanunkul, Maslin
Madesis, Panagiotis
author_facet Osathanunkul, Maslin
Madesis, Panagiotis
author_sort Osathanunkul, Maslin
collection PubMed
description Capture-based aquaculture is now gaining much attention in Southeast Asia. This system was used to produce several fish species with social and economic implications, including the giant snakehead (Channa micropeltes). As wild harvesting of organisms for seed stock is one of main practices in capture-based aquaculture, abundance and distribution of the wild stock are essential for both environmental impact evaluation and stock management. Mark and recapture, visual observation and physical capture of target species are costly, ineffective, and labour intensive for fish surveys in several cases. Detection of target organisms using eDNA (environmental DNA) could be a good alternative as it has proved to be a non-invasive, rapid, and sensitive method for aquatic species monitoring and surveying. Here, we developed a TaqMan assay that targets the 16S region of giant snakehead DNA to amplify eDNA captured in water samples. 300 µl of water samples were collected from 15 sites located in the Chao Phraya River Basin (Ping, Wang, Yom, Nan, and Chao Phraya River) and filtered with 0.7 µm glass fibre membrane filter. Giant snakehead eDNA was detected in most tributaries (60%) with concentrations ranging from 74.0 copies/ml in Wang River sites to 7.4 copies/ml in Nan River sites. As intensification of capture-based aquaculture could lead to depleting of wild fish stocks, urgent management is needed. However, the existing conventional approaches for assessment of fish overexploitation, survey and monitoring have several limitations.
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spelling pubmed-90899102022-05-11 Environmental DNA detection of giant snakehead in Thailand’s major rivers for wild stock assessment Osathanunkul, Maslin Madesis, Panagiotis PLoS One Research Article Capture-based aquaculture is now gaining much attention in Southeast Asia. This system was used to produce several fish species with social and economic implications, including the giant snakehead (Channa micropeltes). As wild harvesting of organisms for seed stock is one of main practices in capture-based aquaculture, abundance and distribution of the wild stock are essential for both environmental impact evaluation and stock management. Mark and recapture, visual observation and physical capture of target species are costly, ineffective, and labour intensive for fish surveys in several cases. Detection of target organisms using eDNA (environmental DNA) could be a good alternative as it has proved to be a non-invasive, rapid, and sensitive method for aquatic species monitoring and surveying. Here, we developed a TaqMan assay that targets the 16S region of giant snakehead DNA to amplify eDNA captured in water samples. 300 µl of water samples were collected from 15 sites located in the Chao Phraya River Basin (Ping, Wang, Yom, Nan, and Chao Phraya River) and filtered with 0.7 µm glass fibre membrane filter. Giant snakehead eDNA was detected in most tributaries (60%) with concentrations ranging from 74.0 copies/ml in Wang River sites to 7.4 copies/ml in Nan River sites. As intensification of capture-based aquaculture could lead to depleting of wild fish stocks, urgent management is needed. However, the existing conventional approaches for assessment of fish overexploitation, survey and monitoring have several limitations. Public Library of Science 2022-05-10 /pmc/articles/PMC9089910/ /pubmed/35536840 http://dx.doi.org/10.1371/journal.pone.0267667 Text en © 2022 Osathanunkul, Madesis https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Osathanunkul, Maslin
Madesis, Panagiotis
Environmental DNA detection of giant snakehead in Thailand’s major rivers for wild stock assessment
title Environmental DNA detection of giant snakehead in Thailand’s major rivers for wild stock assessment
title_full Environmental DNA detection of giant snakehead in Thailand’s major rivers for wild stock assessment
title_fullStr Environmental DNA detection of giant snakehead in Thailand’s major rivers for wild stock assessment
title_full_unstemmed Environmental DNA detection of giant snakehead in Thailand’s major rivers for wild stock assessment
title_short Environmental DNA detection of giant snakehead in Thailand’s major rivers for wild stock assessment
title_sort environmental dna detection of giant snakehead in thailand’s major rivers for wild stock assessment
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089910/
https://www.ncbi.nlm.nih.gov/pubmed/35536840
http://dx.doi.org/10.1371/journal.pone.0267667
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