A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon

Lead is a highly toxic heavy metal, and various functional nucleic acid (FNA)-based biosensors have been developed for the detection of Pb(2+) in environmental monitoring. However, most fluorescence biosensors that have been reported were designed on the basis of a double-labeled (fluorophore and qu...

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Autores principales: Li, Qianyun, Jia, Yongmei, Feng, Zongcai, Liu, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090614/
https://www.ncbi.nlm.nih.gov/pubmed/35558300
http://dx.doi.org/10.1039/c8ra07903j
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author Li, Qianyun
Jia, Yongmei
Feng, Zongcai
Liu, Fang
author_facet Li, Qianyun
Jia, Yongmei
Feng, Zongcai
Liu, Fang
author_sort Li, Qianyun
collection PubMed
description Lead is a highly toxic heavy metal, and various functional nucleic acid (FNA)-based biosensors have been developed for the detection of Pb(2+) in environmental monitoring. However, most fluorescence biosensors that have been reported were designed on the basis of a double-labeled (fluorophore and quencher group) DNA sequence, which not only involved an inconvenient organic synthesis but also restricted their wider use in practical applications. Here, we utilized a G-rich DNA sequence as a recognition probe and conjugated fluorene (CF) to develop a fluorescence sensor without a quencher based on the aggregation-caused quenching (ACQ) effect. In the presence of Pb(2+), the degree of aggregation of CF was reduced because Pb(2+) induced the formation of a G-quadruplex structure of the CF-DNA probe, and the fluorescence signal increased with the concentration of Pb(2+) (0–1 μM), with a limit of detection of 0.36 nM. This fluorescent probe without a quencher enables the sensitive and selective detection of Pb(2+). On the basis of these advantages, the CF-DNA probe represents a promising analytical method for detecting Pb(2+).
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spelling pubmed-90906142022-05-11 A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon Li, Qianyun Jia, Yongmei Feng, Zongcai Liu, Fang RSC Adv Chemistry Lead is a highly toxic heavy metal, and various functional nucleic acid (FNA)-based biosensors have been developed for the detection of Pb(2+) in environmental monitoring. However, most fluorescence biosensors that have been reported were designed on the basis of a double-labeled (fluorophore and quencher group) DNA sequence, which not only involved an inconvenient organic synthesis but also restricted their wider use in practical applications. Here, we utilized a G-rich DNA sequence as a recognition probe and conjugated fluorene (CF) to develop a fluorescence sensor without a quencher based on the aggregation-caused quenching (ACQ) effect. In the presence of Pb(2+), the degree of aggregation of CF was reduced because Pb(2+) induced the formation of a G-quadruplex structure of the CF-DNA probe, and the fluorescence signal increased with the concentration of Pb(2+) (0–1 μM), with a limit of detection of 0.36 nM. This fluorescent probe without a quencher enables the sensitive and selective detection of Pb(2+). On the basis of these advantages, the CF-DNA probe represents a promising analytical method for detecting Pb(2+). The Royal Society of Chemistry 2018-11-19 /pmc/articles/PMC9090614/ /pubmed/35558300 http://dx.doi.org/10.1039/c8ra07903j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Li, Qianyun
Jia, Yongmei
Feng, Zongcai
Liu, Fang
A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon
title A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon
title_full A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon
title_fullStr A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon
title_full_unstemmed A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon
title_short A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon
title_sort highly sensitive and selective fluorescent probe without quencher for detection of pb(2+) ions based on aggregation-caused quenching phenomenon
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090614/
https://www.ncbi.nlm.nih.gov/pubmed/35558300
http://dx.doi.org/10.1039/c8ra07903j
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