A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon
Lead is a highly toxic heavy metal, and various functional nucleic acid (FNA)-based biosensors have been developed for the detection of Pb(2+) in environmental monitoring. However, most fluorescence biosensors that have been reported were designed on the basis of a double-labeled (fluorophore and qu...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090614/ https://www.ncbi.nlm.nih.gov/pubmed/35558300 http://dx.doi.org/10.1039/c8ra07903j |
_version_ | 1784704762529185792 |
---|---|
author | Li, Qianyun Jia, Yongmei Feng, Zongcai Liu, Fang |
author_facet | Li, Qianyun Jia, Yongmei Feng, Zongcai Liu, Fang |
author_sort | Li, Qianyun |
collection | PubMed |
description | Lead is a highly toxic heavy metal, and various functional nucleic acid (FNA)-based biosensors have been developed for the detection of Pb(2+) in environmental monitoring. However, most fluorescence biosensors that have been reported were designed on the basis of a double-labeled (fluorophore and quencher group) DNA sequence, which not only involved an inconvenient organic synthesis but also restricted their wider use in practical applications. Here, we utilized a G-rich DNA sequence as a recognition probe and conjugated fluorene (CF) to develop a fluorescence sensor without a quencher based on the aggregation-caused quenching (ACQ) effect. In the presence of Pb(2+), the degree of aggregation of CF was reduced because Pb(2+) induced the formation of a G-quadruplex structure of the CF-DNA probe, and the fluorescence signal increased with the concentration of Pb(2+) (0–1 μM), with a limit of detection of 0.36 nM. This fluorescent probe without a quencher enables the sensitive and selective detection of Pb(2+). On the basis of these advantages, the CF-DNA probe represents a promising analytical method for detecting Pb(2+). |
format | Online Article Text |
id | pubmed-9090614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90906142022-05-11 A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon Li, Qianyun Jia, Yongmei Feng, Zongcai Liu, Fang RSC Adv Chemistry Lead is a highly toxic heavy metal, and various functional nucleic acid (FNA)-based biosensors have been developed for the detection of Pb(2+) in environmental monitoring. However, most fluorescence biosensors that have been reported were designed on the basis of a double-labeled (fluorophore and quencher group) DNA sequence, which not only involved an inconvenient organic synthesis but also restricted their wider use in practical applications. Here, we utilized a G-rich DNA sequence as a recognition probe and conjugated fluorene (CF) to develop a fluorescence sensor without a quencher based on the aggregation-caused quenching (ACQ) effect. In the presence of Pb(2+), the degree of aggregation of CF was reduced because Pb(2+) induced the formation of a G-quadruplex structure of the CF-DNA probe, and the fluorescence signal increased with the concentration of Pb(2+) (0–1 μM), with a limit of detection of 0.36 nM. This fluorescent probe without a quencher enables the sensitive and selective detection of Pb(2+). On the basis of these advantages, the CF-DNA probe represents a promising analytical method for detecting Pb(2+). The Royal Society of Chemistry 2018-11-19 /pmc/articles/PMC9090614/ /pubmed/35558300 http://dx.doi.org/10.1039/c8ra07903j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Li, Qianyun Jia, Yongmei Feng, Zongcai Liu, Fang A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon |
title | A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon |
title_full | A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon |
title_fullStr | A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon |
title_full_unstemmed | A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon |
title_short | A highly sensitive and selective fluorescent probe without quencher for detection of Pb(2+) ions based on aggregation-caused quenching phenomenon |
title_sort | highly sensitive and selective fluorescent probe without quencher for detection of pb(2+) ions based on aggregation-caused quenching phenomenon |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090614/ https://www.ncbi.nlm.nih.gov/pubmed/35558300 http://dx.doi.org/10.1039/c8ra07903j |
work_keys_str_mv | AT liqianyun ahighlysensitiveandselectivefluorescentprobewithoutquencherfordetectionofpb2ionsbasedonaggregationcausedquenchingphenomenon AT jiayongmei ahighlysensitiveandselectivefluorescentprobewithoutquencherfordetectionofpb2ionsbasedonaggregationcausedquenchingphenomenon AT fengzongcai ahighlysensitiveandselectivefluorescentprobewithoutquencherfordetectionofpb2ionsbasedonaggregationcausedquenchingphenomenon AT liufang ahighlysensitiveandselectivefluorescentprobewithoutquencherfordetectionofpb2ionsbasedonaggregationcausedquenchingphenomenon AT liqianyun highlysensitiveandselectivefluorescentprobewithoutquencherfordetectionofpb2ionsbasedonaggregationcausedquenchingphenomenon AT jiayongmei highlysensitiveandselectivefluorescentprobewithoutquencherfordetectionofpb2ionsbasedonaggregationcausedquenchingphenomenon AT fengzongcai highlysensitiveandselectivefluorescentprobewithoutquencherfordetectionofpb2ionsbasedonaggregationcausedquenchingphenomenon AT liufang highlysensitiveandselectivefluorescentprobewithoutquencherfordetectionofpb2ionsbasedonaggregationcausedquenchingphenomenon |