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An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation

Even though a detailed understanding of the proliferative characteristics of T lymphocytes is imperative in many research fields, prior studies have never reached a consensus on these characteristics, and on the corresponding cell cycle kinetics specifically. In this study, the general proliferative...

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Autores principales: Duthoo, Evi, Vral, Anne, Baeyens, Ans
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090834/
https://www.ncbi.nlm.nih.gov/pubmed/35538107
http://dx.doi.org/10.1038/s41598-022-11364-9
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author Duthoo, Evi
Vral, Anne
Baeyens, Ans
author_facet Duthoo, Evi
Vral, Anne
Baeyens, Ans
author_sort Duthoo, Evi
collection PubMed
description Even though a detailed understanding of the proliferative characteristics of T lymphocytes is imperative in many research fields, prior studies have never reached a consensus on these characteristics, and on the corresponding cell cycle kinetics specifically. In this study, the general proliferative response of human T lymphocytes to phytohaemagglutinin (PHA) stimulation was characterized using a carboxyfluorescein succinimidyl ester-based flow cytometric assay. We were able to determine when PHA-stimulated T lymphocytes complete their first division, the proportion of cells that initiate proliferation, the subsequent division rate of the cells, and the impact of irradiation on these proliferative properties. Next, we accurately visualized the cell cycle progression of dividing T lymphocytes cultured in whole blood using an adapted 5-ethynyl-2’-deoxyuridine pulse-chase method. Furthermore, through multiple downstream analysis methods, we were able to make an estimation of the corresponding cell cycle kinetics. We also visualized the impact of X-rays on the progression of the cells through the cell cycle. Our results showed dose-dependent G2 arrest after exposure to irradiation, and a corresponding delay in G1 phase-entry of the cells. In conclusion, utilizing various flow cytometric assays, we provided valuable information on T lymphocyte proliferation characteristics starting from first division to fully dividing cells.
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spelling pubmed-90908342022-05-12 An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation Duthoo, Evi Vral, Anne Baeyens, Ans Sci Rep Article Even though a detailed understanding of the proliferative characteristics of T lymphocytes is imperative in many research fields, prior studies have never reached a consensus on these characteristics, and on the corresponding cell cycle kinetics specifically. In this study, the general proliferative response of human T lymphocytes to phytohaemagglutinin (PHA) stimulation was characterized using a carboxyfluorescein succinimidyl ester-based flow cytometric assay. We were able to determine when PHA-stimulated T lymphocytes complete their first division, the proportion of cells that initiate proliferation, the subsequent division rate of the cells, and the impact of irradiation on these proliferative properties. Next, we accurately visualized the cell cycle progression of dividing T lymphocytes cultured in whole blood using an adapted 5-ethynyl-2’-deoxyuridine pulse-chase method. Furthermore, through multiple downstream analysis methods, we were able to make an estimation of the corresponding cell cycle kinetics. We also visualized the impact of X-rays on the progression of the cells through the cell cycle. Our results showed dose-dependent G2 arrest after exposure to irradiation, and a corresponding delay in G1 phase-entry of the cells. In conclusion, utilizing various flow cytometric assays, we provided valuable information on T lymphocyte proliferation characteristics starting from first division to fully dividing cells. Nature Publishing Group UK 2022-05-10 /pmc/articles/PMC9090834/ /pubmed/35538107 http://dx.doi.org/10.1038/s41598-022-11364-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Duthoo, Evi
Vral, Anne
Baeyens, Ans
An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation
title An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation
title_full An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation
title_fullStr An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation
title_full_unstemmed An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation
title_short An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation
title_sort updated view into the cell cycle kinetics of human t lymphocytes and the impact of irradiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090834/
https://www.ncbi.nlm.nih.gov/pubmed/35538107
http://dx.doi.org/10.1038/s41598-022-11364-9
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