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Metagenomic sequencing characterizes a wide diversity of viruses in field mosquito samples in Nigeria

Mosquito vectors are a tremendous public health threat. One in six diseases worldwide is vector-borne transmitted mainly by mosquitoes. In the last couple of years, there have been active Yellow fever virus (YFV) outbreaks in many settings in Nigeria, and nationwide, entomological surveillance has b...

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Autores principales: Oguzie, Judith U., Nwangwu, Udoka C., Oluniyi, Paul E., Olumade, Testimony J., George, Uwem E., Kazeem, Akano, Bankole, Bolajoko E., Brimmo, Farida O., Asadu, Chukwuemeka C., Chukwuekezie, Okechukwu C., Ochu, Josephine C., Makwe, Catherine O., Dogunro, Festus A., Onwude, Cosmas O., Nwachukwu, William E., Ezihe, Ebuka K., Okonkwo, Gilkenny K., Umazi, Ndubuisi E., Maikere, Jacob, Agashi, Nneka O., Eloy, Emelda I., Anokwu, Stephen O., Okoronkwo, Angela I., Nwosu, Ebuka M., Etiki, Sandra O., Ngwu, Ifeoma M., Ihekweazu, Chikwe, Folarin, Onikepe A., Komolafe, Isaac O. O., Happi, Christian T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090917/
https://www.ncbi.nlm.nih.gov/pubmed/35538241
http://dx.doi.org/10.1038/s41598-022-11797-2
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author Oguzie, Judith U.
Nwangwu, Udoka C.
Oluniyi, Paul E.
Olumade, Testimony J.
George, Uwem E.
Kazeem, Akano
Bankole, Bolajoko E.
Brimmo, Farida O.
Asadu, Chukwuemeka C.
Chukwuekezie, Okechukwu C.
Ochu, Josephine C.
Makwe, Catherine O.
Dogunro, Festus A.
Onwude, Cosmas O.
Nwachukwu, William E.
Ezihe, Ebuka K.
Okonkwo, Gilkenny K.
Umazi, Ndubuisi E.
Maikere, Jacob
Agashi, Nneka O.
Eloy, Emelda I.
Anokwu, Stephen O.
Okoronkwo, Angela I.
Nwosu, Ebuka M.
Etiki, Sandra O.
Ngwu, Ifeoma M.
Ihekweazu, Chikwe
Folarin, Onikepe A.
Komolafe, Isaac O. O.
Happi, Christian T.
author_facet Oguzie, Judith U.
Nwangwu, Udoka C.
Oluniyi, Paul E.
Olumade, Testimony J.
George, Uwem E.
Kazeem, Akano
Bankole, Bolajoko E.
Brimmo, Farida O.
Asadu, Chukwuemeka C.
Chukwuekezie, Okechukwu C.
Ochu, Josephine C.
Makwe, Catherine O.
Dogunro, Festus A.
Onwude, Cosmas O.
Nwachukwu, William E.
Ezihe, Ebuka K.
Okonkwo, Gilkenny K.
Umazi, Ndubuisi E.
Maikere, Jacob
Agashi, Nneka O.
Eloy, Emelda I.
Anokwu, Stephen O.
Okoronkwo, Angela I.
Nwosu, Ebuka M.
Etiki, Sandra O.
Ngwu, Ifeoma M.
Ihekweazu, Chikwe
Folarin, Onikepe A.
Komolafe, Isaac O. O.
Happi, Christian T.
author_sort Oguzie, Judith U.
collection PubMed
description Mosquito vectors are a tremendous public health threat. One in six diseases worldwide is vector-borne transmitted mainly by mosquitoes. In the last couple of years, there have been active Yellow fever virus (YFV) outbreaks in many settings in Nigeria, and nationwide, entomological surveillance has been a significant effort geared towards understanding these outbreaks. In this study, we used a metagenomic sequencing approach to characterize viruses present in vector samples collected during various outbreaks of Yellow fever (YF) in Nigeria between 2017 and 2020. Mosquito samples were grouped into pools of 1 to 50 mosquitoes, each based on species, sex and location. Twenty-five pools of Aedes spp and one pool of Anopheles spp collected from nine states were sequenced and metagenomic analysis was carried out. We identified a wide diversity of viruses belonging to various families in this sample set. Seven different viruses detected included: Fako virus, Phasi Charoen-like virus, Verdadero virus, Chaq like-virus, Aedes aegypti totivirus, cell fusing agent virus and Tesano Aedes virus. Although there are no reports of these viruses being pathogenic, they are an understudied group in the same families and closely related to known pathogenic arboviruses. Our study highlights the power of next generation sequencing in identifying Insect specific viruses (ISVs), and provide insight into mosquito vectors virome in Nigeria.
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spelling pubmed-90909172022-05-12 Metagenomic sequencing characterizes a wide diversity of viruses in field mosquito samples in Nigeria Oguzie, Judith U. Nwangwu, Udoka C. Oluniyi, Paul E. Olumade, Testimony J. George, Uwem E. Kazeem, Akano Bankole, Bolajoko E. Brimmo, Farida O. Asadu, Chukwuemeka C. Chukwuekezie, Okechukwu C. Ochu, Josephine C. Makwe, Catherine O. Dogunro, Festus A. Onwude, Cosmas O. Nwachukwu, William E. Ezihe, Ebuka K. Okonkwo, Gilkenny K. Umazi, Ndubuisi E. Maikere, Jacob Agashi, Nneka O. Eloy, Emelda I. Anokwu, Stephen O. Okoronkwo, Angela I. Nwosu, Ebuka M. Etiki, Sandra O. Ngwu, Ifeoma M. Ihekweazu, Chikwe Folarin, Onikepe A. Komolafe, Isaac O. O. Happi, Christian T. Sci Rep Article Mosquito vectors are a tremendous public health threat. One in six diseases worldwide is vector-borne transmitted mainly by mosquitoes. In the last couple of years, there have been active Yellow fever virus (YFV) outbreaks in many settings in Nigeria, and nationwide, entomological surveillance has been a significant effort geared towards understanding these outbreaks. In this study, we used a metagenomic sequencing approach to characterize viruses present in vector samples collected during various outbreaks of Yellow fever (YF) in Nigeria between 2017 and 2020. Mosquito samples were grouped into pools of 1 to 50 mosquitoes, each based on species, sex and location. Twenty-five pools of Aedes spp and one pool of Anopheles spp collected from nine states were sequenced and metagenomic analysis was carried out. We identified a wide diversity of viruses belonging to various families in this sample set. Seven different viruses detected included: Fako virus, Phasi Charoen-like virus, Verdadero virus, Chaq like-virus, Aedes aegypti totivirus, cell fusing agent virus and Tesano Aedes virus. Although there are no reports of these viruses being pathogenic, they are an understudied group in the same families and closely related to known pathogenic arboviruses. Our study highlights the power of next generation sequencing in identifying Insect specific viruses (ISVs), and provide insight into mosquito vectors virome in Nigeria. Nature Publishing Group UK 2022-05-10 /pmc/articles/PMC9090917/ /pubmed/35538241 http://dx.doi.org/10.1038/s41598-022-11797-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Oguzie, Judith U.
Nwangwu, Udoka C.
Oluniyi, Paul E.
Olumade, Testimony J.
George, Uwem E.
Kazeem, Akano
Bankole, Bolajoko E.
Brimmo, Farida O.
Asadu, Chukwuemeka C.
Chukwuekezie, Okechukwu C.
Ochu, Josephine C.
Makwe, Catherine O.
Dogunro, Festus A.
Onwude, Cosmas O.
Nwachukwu, William E.
Ezihe, Ebuka K.
Okonkwo, Gilkenny K.
Umazi, Ndubuisi E.
Maikere, Jacob
Agashi, Nneka O.
Eloy, Emelda I.
Anokwu, Stephen O.
Okoronkwo, Angela I.
Nwosu, Ebuka M.
Etiki, Sandra O.
Ngwu, Ifeoma M.
Ihekweazu, Chikwe
Folarin, Onikepe A.
Komolafe, Isaac O. O.
Happi, Christian T.
Metagenomic sequencing characterizes a wide diversity of viruses in field mosquito samples in Nigeria
title Metagenomic sequencing characterizes a wide diversity of viruses in field mosquito samples in Nigeria
title_full Metagenomic sequencing characterizes a wide diversity of viruses in field mosquito samples in Nigeria
title_fullStr Metagenomic sequencing characterizes a wide diversity of viruses in field mosquito samples in Nigeria
title_full_unstemmed Metagenomic sequencing characterizes a wide diversity of viruses in field mosquito samples in Nigeria
title_short Metagenomic sequencing characterizes a wide diversity of viruses in field mosquito samples in Nigeria
title_sort metagenomic sequencing characterizes a wide diversity of viruses in field mosquito samples in nigeria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090917/
https://www.ncbi.nlm.nih.gov/pubmed/35538241
http://dx.doi.org/10.1038/s41598-022-11797-2
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