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Efficient production of sugar-derived aldonic acids by Pseudomonas fragi TCCC11892

Aldonic acids are receiving increased interest due to their applications in nanotechnology, food, pharmaceutical and chemical industries. Microbes with aldose-oxidizing activity, rather than purified enzymes, are used for commercial production with limited success. Thus it is still very important to...

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Autores principales: Mao, Shuhong, Liu, Yanna, Hou, Yali, Ma, Xiaoyu, Yang, Juanjuan, Han, Haichao, Wu, Jianlin, Jia, Longgang, Qin, Huimin, Lu, Fuping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091311/
https://www.ncbi.nlm.nih.gov/pubmed/35558231
http://dx.doi.org/10.1039/c8ra07556e
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author Mao, Shuhong
Liu, Yanna
Hou, Yali
Ma, Xiaoyu
Yang, Juanjuan
Han, Haichao
Wu, Jianlin
Jia, Longgang
Qin, Huimin
Lu, Fuping
author_facet Mao, Shuhong
Liu, Yanna
Hou, Yali
Ma, Xiaoyu
Yang, Juanjuan
Han, Haichao
Wu, Jianlin
Jia, Longgang
Qin, Huimin
Lu, Fuping
author_sort Mao, Shuhong
collection PubMed
description Aldonic acids are receiving increased interest due to their applications in nanotechnology, food, pharmaceutical and chemical industries. Microbes with aldose-oxidizing activity, rather than purified enzymes, are used for commercial production with limited success. Thus it is still very important to develop new processes using strains with more efficient and novel biocatalytic activities for the production of adonic acids. In the present study, Pseudomonas fragi TCCC11892 was found to be an efficient producer of aldonic acids, with the production of galactonic and l-rhamnonic acid by P. fragi reported for the first time. The semi-continuous production of maltobionic acid and lactobionic acid was developed for P. fragi TCCC11892, achieving a yield of over 90 g L(−1) for the first 7 cycles. The excellent performance of P. fragi in the production of lactobionic acid (119 g L(−1)) was also observed when using waste cheese whey as an inexpensive fermentation medium. Scaling up of the above process for production of aldonic acids with P. fragi TCCC11892 cells should facilitate their commercial applications.
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spelling pubmed-90913112022-05-11 Efficient production of sugar-derived aldonic acids by Pseudomonas fragi TCCC11892 Mao, Shuhong Liu, Yanna Hou, Yali Ma, Xiaoyu Yang, Juanjuan Han, Haichao Wu, Jianlin Jia, Longgang Qin, Huimin Lu, Fuping RSC Adv Chemistry Aldonic acids are receiving increased interest due to their applications in nanotechnology, food, pharmaceutical and chemical industries. Microbes with aldose-oxidizing activity, rather than purified enzymes, are used for commercial production with limited success. Thus it is still very important to develop new processes using strains with more efficient and novel biocatalytic activities for the production of adonic acids. In the present study, Pseudomonas fragi TCCC11892 was found to be an efficient producer of aldonic acids, with the production of galactonic and l-rhamnonic acid by P. fragi reported for the first time. The semi-continuous production of maltobionic acid and lactobionic acid was developed for P. fragi TCCC11892, achieving a yield of over 90 g L(−1) for the first 7 cycles. The excellent performance of P. fragi in the production of lactobionic acid (119 g L(−1)) was also observed when using waste cheese whey as an inexpensive fermentation medium. Scaling up of the above process for production of aldonic acids with P. fragi TCCC11892 cells should facilitate their commercial applications. The Royal Society of Chemistry 2018-11-30 /pmc/articles/PMC9091311/ /pubmed/35558231 http://dx.doi.org/10.1039/c8ra07556e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Mao, Shuhong
Liu, Yanna
Hou, Yali
Ma, Xiaoyu
Yang, Juanjuan
Han, Haichao
Wu, Jianlin
Jia, Longgang
Qin, Huimin
Lu, Fuping
Efficient production of sugar-derived aldonic acids by Pseudomonas fragi TCCC11892
title Efficient production of sugar-derived aldonic acids by Pseudomonas fragi TCCC11892
title_full Efficient production of sugar-derived aldonic acids by Pseudomonas fragi TCCC11892
title_fullStr Efficient production of sugar-derived aldonic acids by Pseudomonas fragi TCCC11892
title_full_unstemmed Efficient production of sugar-derived aldonic acids by Pseudomonas fragi TCCC11892
title_short Efficient production of sugar-derived aldonic acids by Pseudomonas fragi TCCC11892
title_sort efficient production of sugar-derived aldonic acids by pseudomonas fragi tccc11892
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091311/
https://www.ncbi.nlm.nih.gov/pubmed/35558231
http://dx.doi.org/10.1039/c8ra07556e
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