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An ultrasensitive fluorescent aptasensor for detection of cancer marker proteins based on graphene oxide–ssDNA

A novel biosensing platform was developed by integrating a new ssDNA aptamer and graphene oxide (GO) for highly sensitive and selective detection of liver cancer biomarkers (alpha-fetoprotein, AFP). The key concept of this biosensing platform is that the fluorescence of dye-modified ssDNA can be eff...

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Autores principales: Wang, Chen-Feng, Wang, Zhen-Guang, Sun, Xin-Yue, Chen, Meng-Jiao, Lv, Yun-Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091683/
https://www.ncbi.nlm.nih.gov/pubmed/35559324
http://dx.doi.org/10.1039/c8ra08078j
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author Wang, Chen-Feng
Wang, Zhen-Guang
Sun, Xin-Yue
Chen, Meng-Jiao
Lv, Yun-Kai
author_facet Wang, Chen-Feng
Wang, Zhen-Guang
Sun, Xin-Yue
Chen, Meng-Jiao
Lv, Yun-Kai
author_sort Wang, Chen-Feng
collection PubMed
description A novel biosensing platform was developed by integrating a new ssDNA aptamer and graphene oxide (GO) for highly sensitive and selective detection of liver cancer biomarkers (alpha-fetoprotein, AFP). The key concept of this biosensing platform is that the fluorescence of dye-modified ssDNA can be effectively quenched by GO after forming the hybrid structure of graphene oxide–ssDNA (GO–ssDNA). The AFP can selectively react with GO–ssDNA and lead to the decomposition of GO–ssDNA, which results in the recovery of fluorescence, and an increase in fluorescence intensity with the increasing concentration of AFP in the range of 0 to 300 pg mL(−1). The linear range was obtained from 1 to 150 pg mL(−1) and the detection limit was 0.909 pg mL(−1). Moreover, this biosensing platform can be applied to serum and cell imaging for the detection of AFP. The results show that the proposed biosensor has great potential application in AFP-related clinical diagnosis and research.
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spelling pubmed-90916832022-05-11 An ultrasensitive fluorescent aptasensor for detection of cancer marker proteins based on graphene oxide–ssDNA Wang, Chen-Feng Wang, Zhen-Guang Sun, Xin-Yue Chen, Meng-Jiao Lv, Yun-Kai RSC Adv Chemistry A novel biosensing platform was developed by integrating a new ssDNA aptamer and graphene oxide (GO) for highly sensitive and selective detection of liver cancer biomarkers (alpha-fetoprotein, AFP). The key concept of this biosensing platform is that the fluorescence of dye-modified ssDNA can be effectively quenched by GO after forming the hybrid structure of graphene oxide–ssDNA (GO–ssDNA). The AFP can selectively react with GO–ssDNA and lead to the decomposition of GO–ssDNA, which results in the recovery of fluorescence, and an increase in fluorescence intensity with the increasing concentration of AFP in the range of 0 to 300 pg mL(−1). The linear range was obtained from 1 to 150 pg mL(−1) and the detection limit was 0.909 pg mL(−1). Moreover, this biosensing platform can be applied to serum and cell imaging for the detection of AFP. The results show that the proposed biosensor has great potential application in AFP-related clinical diagnosis and research. The Royal Society of Chemistry 2018-12-07 /pmc/articles/PMC9091683/ /pubmed/35559324 http://dx.doi.org/10.1039/c8ra08078j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Wang, Chen-Feng
Wang, Zhen-Guang
Sun, Xin-Yue
Chen, Meng-Jiao
Lv, Yun-Kai
An ultrasensitive fluorescent aptasensor for detection of cancer marker proteins based on graphene oxide–ssDNA
title An ultrasensitive fluorescent aptasensor for detection of cancer marker proteins based on graphene oxide–ssDNA
title_full An ultrasensitive fluorescent aptasensor for detection of cancer marker proteins based on graphene oxide–ssDNA
title_fullStr An ultrasensitive fluorescent aptasensor for detection of cancer marker proteins based on graphene oxide–ssDNA
title_full_unstemmed An ultrasensitive fluorescent aptasensor for detection of cancer marker proteins based on graphene oxide–ssDNA
title_short An ultrasensitive fluorescent aptasensor for detection of cancer marker proteins based on graphene oxide–ssDNA
title_sort ultrasensitive fluorescent aptasensor for detection of cancer marker proteins based on graphene oxide–ssdna
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091683/
https://www.ncbi.nlm.nih.gov/pubmed/35559324
http://dx.doi.org/10.1039/c8ra08078j
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