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Missense Variant rs28362680 in BTNL2 Reduces Risk of Coronary Heart Disease

BACKGROUND: The pathological basis of coronary heart disease (CHD) is atherosclerosis. BTNL2 can inhibit the activation of T cells. We aimed to explore the association between BTNL2 genetic variants and CHD risk in the southern Chinese Han population. METHODS: We recruited 1419 participants to perfo...

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Autores principales: Zhuo, Jian, Wu, Yingchun, Li, Wei, Li, Zerong, Ding, Yipeng, Jin, Tianbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091699/
https://www.ncbi.nlm.nih.gov/pubmed/35572349
http://dx.doi.org/10.2147/PGPM.S353085
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author Zhuo, Jian
Wu, Yingchun
Li, Wei
Li, Zerong
Ding, Yipeng
Jin, Tianbo
author_facet Zhuo, Jian
Wu, Yingchun
Li, Wei
Li, Zerong
Ding, Yipeng
Jin, Tianbo
author_sort Zhuo, Jian
collection PubMed
description BACKGROUND: The pathological basis of coronary heart disease (CHD) is atherosclerosis. BTNL2 can inhibit the activation of T cells. We aimed to explore the association between BTNL2 genetic variants and CHD risk in the southern Chinese Han population. METHODS: We recruited 1419 participants to perform an association analysis between missense variants in BTNL2 and CHD risk through SNPStats online software. Genotyping of all candidate SNPs were completed by the Agena MassARRAY. In addition, we used false-positive report probability analysis to detect whether the positive findings were noteworthy observations. We also used Haploview 4.2 software and SNPStats online software to conduct the haplotype analysis and analysis of linkage disequilibrium (LD). Finally, the interaction of SNP-SNP in CHD risk was evaluated by multi-factor dimensionality reduction (MDR). RESULTS: The results showed that BTNL2-rs35624343, -rs117896888, -rs41441651, -rs41417449, -rs28362680 and -rs2076523 were significantly associated with the CHD susceptibility. Especially for BTNL2-rs28362680, the allele A (OR = 0.68, p < 0.0001), genotype AA (OR = 0.40, p = 0.001) or GA (OR = 0.68, p < 0.0001) were associated with the reducing CHD risk. And -rs28362680 significantly reduced the CHD risk under all genetic models (dominant: OR = 0.64, p < 0.0001; recessive: OR = 0.47, p = 0.003; overdominant: OR = 0.73, p = 0.004; log-additive: OR = 0.66, p < 0.0001). And -rs28362680 was also closely associated with CHD risk reduction in all stratified analyses (age, gender, smoking, drinking, hypertension and diabetes). In addition, haplotype analysis showed that the “C(rs117896888)C(rs41441651)T(rs41417449)A(rs28362680)” (OR = 0.65, p < 0.0001) and “G(rs117896888)T(rs41441651)C(rs41417449)A(rs28362680)” (OR = 0.68, p = 0.013) may reduce CHD risk. CONCLUSION: Missense variants (rs35624343, rs117896888, rs41441651, rs41417449, rs28362680, rs2076523) may be protective factors for the CHD risk. In particular, there were sufficient evidences that BTNL2-rs28362680 can protective CHD risk.
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spelling pubmed-90916992022-05-12 Missense Variant rs28362680 in BTNL2 Reduces Risk of Coronary Heart Disease Zhuo, Jian Wu, Yingchun Li, Wei Li, Zerong Ding, Yipeng Jin, Tianbo Pharmgenomics Pers Med Original Research BACKGROUND: The pathological basis of coronary heart disease (CHD) is atherosclerosis. BTNL2 can inhibit the activation of T cells. We aimed to explore the association between BTNL2 genetic variants and CHD risk in the southern Chinese Han population. METHODS: We recruited 1419 participants to perform an association analysis between missense variants in BTNL2 and CHD risk through SNPStats online software. Genotyping of all candidate SNPs were completed by the Agena MassARRAY. In addition, we used false-positive report probability analysis to detect whether the positive findings were noteworthy observations. We also used Haploview 4.2 software and SNPStats online software to conduct the haplotype analysis and analysis of linkage disequilibrium (LD). Finally, the interaction of SNP-SNP in CHD risk was evaluated by multi-factor dimensionality reduction (MDR). RESULTS: The results showed that BTNL2-rs35624343, -rs117896888, -rs41441651, -rs41417449, -rs28362680 and -rs2076523 were significantly associated with the CHD susceptibility. Especially for BTNL2-rs28362680, the allele A (OR = 0.68, p < 0.0001), genotype AA (OR = 0.40, p = 0.001) or GA (OR = 0.68, p < 0.0001) were associated with the reducing CHD risk. And -rs28362680 significantly reduced the CHD risk under all genetic models (dominant: OR = 0.64, p < 0.0001; recessive: OR = 0.47, p = 0.003; overdominant: OR = 0.73, p = 0.004; log-additive: OR = 0.66, p < 0.0001). And -rs28362680 was also closely associated with CHD risk reduction in all stratified analyses (age, gender, smoking, drinking, hypertension and diabetes). In addition, haplotype analysis showed that the “C(rs117896888)C(rs41441651)T(rs41417449)A(rs28362680)” (OR = 0.65, p < 0.0001) and “G(rs117896888)T(rs41441651)C(rs41417449)A(rs28362680)” (OR = 0.68, p = 0.013) may reduce CHD risk. CONCLUSION: Missense variants (rs35624343, rs117896888, rs41441651, rs41417449, rs28362680, rs2076523) may be protective factors for the CHD risk. In particular, there were sufficient evidences that BTNL2-rs28362680 can protective CHD risk. Dove 2022-05-06 /pmc/articles/PMC9091699/ /pubmed/35572349 http://dx.doi.org/10.2147/PGPM.S353085 Text en © 2022 Zhuo et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Zhuo, Jian
Wu, Yingchun
Li, Wei
Li, Zerong
Ding, Yipeng
Jin, Tianbo
Missense Variant rs28362680 in BTNL2 Reduces Risk of Coronary Heart Disease
title Missense Variant rs28362680 in BTNL2 Reduces Risk of Coronary Heart Disease
title_full Missense Variant rs28362680 in BTNL2 Reduces Risk of Coronary Heart Disease
title_fullStr Missense Variant rs28362680 in BTNL2 Reduces Risk of Coronary Heart Disease
title_full_unstemmed Missense Variant rs28362680 in BTNL2 Reduces Risk of Coronary Heart Disease
title_short Missense Variant rs28362680 in BTNL2 Reduces Risk of Coronary Heart Disease
title_sort missense variant rs28362680 in btnl2 reduces risk of coronary heart disease
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091699/
https://www.ncbi.nlm.nih.gov/pubmed/35572349
http://dx.doi.org/10.2147/PGPM.S353085
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