Surface-coated magnetic nanostructured materials for robust bio-catalysis and biomedical applications-A review

BACKGROUND: Enzymes based bio-catalysis has wide range of applications in various chemical and biological processes. Thus, the process of enzymes immobilization on suitable support to obtain highly active and stable bio-catalysts has great potential in industrial applications. Particularly, surface-modified magnetic nanomaterials have garnered a special interest as versatile platforms for biomolecules/enzyme immobilization. AIM OF REVIEW: This review spotlights recent progress in the immobilization of various enzymes onto surface-coated multifunctional magnetic nanostructured materials and their derived nano-constructs for multiple applications. Conclusive remarks, technical challenges, and insightful opinions on this field of research which are helpful to expand the application prospects of these materials are also given with suitable examples. KEY SCIENTIFIC CONCEPTS OF REVIEW: Nanostructured materials, including surface-coated magnetic nanoparticles have recently gained immense significance as suitable support materials for enzyme immobilization, due to their large surface area, unique functionalities, and high chemical and mechanical stability. Besides, magnetic nanoparticles are less expensive and offers great potential in industrial applications due to their easy recovery and separation form their enzyme conjugates with an external magnetic field. Magnetic nanoparticles based biocatalytic systems offer a wide-working temperature, pH range, increased storage and thermal stabilities. So far, several studies have documented the application of a variety of surface modification and functionalization techniques to circumvent the aggregation and oxidation of magnetic nanoparticles. Surface engineering of magnetic nanoparticles (MNPs) helps to improve the dispersion stability, enhance mechanical and physicochemical properties, upgrade the surface activity and also increases enzyme immobilization capabilities and biocompatibility of the materials. However, several challenges still need to be addressed, such as controlled synthesis of MNPs and clinical aspects of these materials require consistent research from multidisciplinary scientists to realize its practical applications.