Cargando…
Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity
A novel water soluble polysaccharide from dragon fruit peel named DFPWSP-1 was isolated and purified and chemical analysis was performed. The results indicated that DFPWSP-1, with an average molecular weight of 2.98 × 10(2) kDa, mainly contained galacturonic acid, glucose and galactose. Then, a Box–...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092055/ https://www.ncbi.nlm.nih.gov/pubmed/35558788 http://dx.doi.org/10.1039/c8ra06449k |
_version_ | 1784705060770414592 |
---|---|
author | Qian, Shiquan Fang, Xiaohui Dan, Demiao Diao, Enjie Lu, Zhaoxin |
author_facet | Qian, Shiquan Fang, Xiaohui Dan, Demiao Diao, Enjie Lu, Zhaoxin |
author_sort | Qian, Shiquan |
collection | PubMed |
description | A novel water soluble polysaccharide from dragon fruit peel named DFPWSP-1 was isolated and purified and chemical analysis was performed. The results indicated that DFPWSP-1, with an average molecular weight of 2.98 × 10(2) kDa, mainly contained galacturonic acid, glucose and galactose. Then, a Box–Behnken design (BBD) was employed to optimize the ultrasonic-assisted enzymatic extraction (UAEE) of DFPWSP-1. The optimal extraction conditions for the maximum yield of DFPWSP-1 were a cellulase volume of 104 U, an enzymolysis time of 2.06 h, an ultrasonication power of 105 W and a ratio of solution to sample of 8.5 mL g(−1). Under these conditions, the extraction yield of DFPWSP-1 was 20.28%. Furthermore, the polysaccharide DFPWSP-1 exhibited a significant scavenging activity of 2-diphenyl-picrylhydrazyl (DPPH) radical, superoxide anion and hydroxyl radical. DFPWSP-1 may be a potential natural antioxidant in the food industry. |
format | Online Article Text |
id | pubmed-9092055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90920552022-05-11 Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity Qian, Shiquan Fang, Xiaohui Dan, Demiao Diao, Enjie Lu, Zhaoxin RSC Adv Chemistry A novel water soluble polysaccharide from dragon fruit peel named DFPWSP-1 was isolated and purified and chemical analysis was performed. The results indicated that DFPWSP-1, with an average molecular weight of 2.98 × 10(2) kDa, mainly contained galacturonic acid, glucose and galactose. Then, a Box–Behnken design (BBD) was employed to optimize the ultrasonic-assisted enzymatic extraction (UAEE) of DFPWSP-1. The optimal extraction conditions for the maximum yield of DFPWSP-1 were a cellulase volume of 104 U, an enzymolysis time of 2.06 h, an ultrasonication power of 105 W and a ratio of solution to sample of 8.5 mL g(−1). Under these conditions, the extraction yield of DFPWSP-1 was 20.28%. Furthermore, the polysaccharide DFPWSP-1 exhibited a significant scavenging activity of 2-diphenyl-picrylhydrazyl (DPPH) radical, superoxide anion and hydroxyl radical. DFPWSP-1 may be a potential natural antioxidant in the food industry. The Royal Society of Chemistry 2018-12-18 /pmc/articles/PMC9092055/ /pubmed/35558788 http://dx.doi.org/10.1039/c8ra06449k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Qian, Shiquan Fang, Xiaohui Dan, Demiao Diao, Enjie Lu, Zhaoxin Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity |
title | Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity |
title_full | Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity |
title_fullStr | Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity |
title_full_unstemmed | Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity |
title_short | Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity |
title_sort | ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092055/ https://www.ncbi.nlm.nih.gov/pubmed/35558788 http://dx.doi.org/10.1039/c8ra06449k |
work_keys_str_mv | AT qianshiquan ultrasonicassistedenzymaticextractionofawatersolublepolysaccharidefromdragonfruitpeelanditsantioxidantactivity AT fangxiaohui ultrasonicassistedenzymaticextractionofawatersolublepolysaccharidefromdragonfruitpeelanditsantioxidantactivity AT dandemiao ultrasonicassistedenzymaticextractionofawatersolublepolysaccharidefromdragonfruitpeelanditsantioxidantactivity AT diaoenjie ultrasonicassistedenzymaticextractionofawatersolublepolysaccharidefromdragonfruitpeelanditsantioxidantactivity AT luzhaoxin ultrasonicassistedenzymaticextractionofawatersolublepolysaccharidefromdragonfruitpeelanditsantioxidantactivity |