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Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity

A novel water soluble polysaccharide from dragon fruit peel named DFPWSP-1 was isolated and purified and chemical analysis was performed. The results indicated that DFPWSP-1, with an average molecular weight of 2.98 × 10(2) kDa, mainly contained galacturonic acid, glucose and galactose. Then, a Box–...

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Detalles Bibliográficos
Autores principales: Qian, Shiquan, Fang, Xiaohui, Dan, Demiao, Diao, Enjie, Lu, Zhaoxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092055/
https://www.ncbi.nlm.nih.gov/pubmed/35558788
http://dx.doi.org/10.1039/c8ra06449k
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author Qian, Shiquan
Fang, Xiaohui
Dan, Demiao
Diao, Enjie
Lu, Zhaoxin
author_facet Qian, Shiquan
Fang, Xiaohui
Dan, Demiao
Diao, Enjie
Lu, Zhaoxin
author_sort Qian, Shiquan
collection PubMed
description A novel water soluble polysaccharide from dragon fruit peel named DFPWSP-1 was isolated and purified and chemical analysis was performed. The results indicated that DFPWSP-1, with an average molecular weight of 2.98 × 10(2) kDa, mainly contained galacturonic acid, glucose and galactose. Then, a Box–Behnken design (BBD) was employed to optimize the ultrasonic-assisted enzymatic extraction (UAEE) of DFPWSP-1. The optimal extraction conditions for the maximum yield of DFPWSP-1 were a cellulase volume of 104 U, an enzymolysis time of 2.06 h, an ultrasonication power of 105 W and a ratio of solution to sample of 8.5 mL g(−1). Under these conditions, the extraction yield of DFPWSP-1 was 20.28%. Furthermore, the polysaccharide DFPWSP-1 exhibited a significant scavenging activity of 2-diphenyl-picrylhydrazyl (DPPH) radical, superoxide anion and hydroxyl radical. DFPWSP-1 may be a potential natural antioxidant in the food industry.
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spelling pubmed-90920552022-05-11 Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity Qian, Shiquan Fang, Xiaohui Dan, Demiao Diao, Enjie Lu, Zhaoxin RSC Adv Chemistry A novel water soluble polysaccharide from dragon fruit peel named DFPWSP-1 was isolated and purified and chemical analysis was performed. The results indicated that DFPWSP-1, with an average molecular weight of 2.98 × 10(2) kDa, mainly contained galacturonic acid, glucose and galactose. Then, a Box–Behnken design (BBD) was employed to optimize the ultrasonic-assisted enzymatic extraction (UAEE) of DFPWSP-1. The optimal extraction conditions for the maximum yield of DFPWSP-1 were a cellulase volume of 104 U, an enzymolysis time of 2.06 h, an ultrasonication power of 105 W and a ratio of solution to sample of 8.5 mL g(−1). Under these conditions, the extraction yield of DFPWSP-1 was 20.28%. Furthermore, the polysaccharide DFPWSP-1 exhibited a significant scavenging activity of 2-diphenyl-picrylhydrazyl (DPPH) radical, superoxide anion and hydroxyl radical. DFPWSP-1 may be a potential natural antioxidant in the food industry. The Royal Society of Chemistry 2018-12-18 /pmc/articles/PMC9092055/ /pubmed/35558788 http://dx.doi.org/10.1039/c8ra06449k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Qian, Shiquan
Fang, Xiaohui
Dan, Demiao
Diao, Enjie
Lu, Zhaoxin
Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity
title Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity
title_full Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity
title_fullStr Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity
title_full_unstemmed Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity
title_short Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity
title_sort ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092055/
https://www.ncbi.nlm.nih.gov/pubmed/35558788
http://dx.doi.org/10.1039/c8ra06449k
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