Cargando…
DNA fiber combing protocol using in-house reagents and coverslips to analyze replication fork dynamics in mammalian cells
DNA fiber combing is a versatile technique that provides insight into replication fork dynamics at single-molecule resolution. DNA fibers are bound to silanized coverslips and combed, which straightens and aligns the fibers along a single axis. Here, we present a DNA fiber combing protocol that does...
Autores principales: | Moore, Gemma, Jimenez Sainz, Judit, Jensen, Ryan B. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092994/ https://www.ncbi.nlm.nih.gov/pubmed/35573479 http://dx.doi.org/10.1016/j.xpro.2022.101371 |
Ejemplares similares
-
DNA replication profiling by molecular combing on single DNA fibers
por: Fu, Haiqing, et al.
Publicado: (2022) -
DNA molecular combing-based replication fork directionality profiling
por: Blin, Marion, et al.
Publicado: (2021) -
Replication fork rescue in mammalian mitochondria
por: Torregrosa-Muñumer, Rubén, et al.
Publicado: (2019) -
DNA Replication Origins and Fork Progression at Mammalian Telomeres
por: Higa, Mitsunori, et al.
Publicado: (2017) -
EMBEDDING AID FOR COVERSLIP MOUNTS IN ELECTRON MICROSCOPY
por: Nebel, B. R., et al.
Publicado: (1956)