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Modeling Human Prostate Cancer Metastasis in Mice via Resection of Subcutaneous Allografts

The 5-year survival rate for patients diagnosed with distant metastatic prostate cancer in the United States is 30.6%. Therefore, there is a great need to develop in vivo model systems to study prostate cancer metastasis and to test potential therapeutics. Most murine prostate cancer metastatic mode...

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Autores principales: Peiffer, Lauren B., Hicks, Jessica, Sosa, Rebecca Y., De Marzo, Angelo M., Sfanos, Karen S., Maynard, Janielle P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9093182/
https://www.ncbi.nlm.nih.gov/pubmed/35574356
http://dx.doi.org/10.3389/fonc.2022.877536
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author Peiffer, Lauren B.
Hicks, Jessica
Sosa, Rebecca Y.
De Marzo, Angelo M.
Sfanos, Karen S.
Maynard, Janielle P.
author_facet Peiffer, Lauren B.
Hicks, Jessica
Sosa, Rebecca Y.
De Marzo, Angelo M.
Sfanos, Karen S.
Maynard, Janielle P.
author_sort Peiffer, Lauren B.
collection PubMed
description The 5-year survival rate for patients diagnosed with distant metastatic prostate cancer in the United States is 30.6%. Therefore, there is a great need to develop in vivo model systems to study prostate cancer metastasis and to test potential therapeutics. Most murine prostate cancer metastatic models involve intracardiac or intraosseous implantation of cancer cells, which bypass the early stages of tumor cell migration and invasion. Herein we provide a detailed protocol for a novel method of resecting subcutaneous prostate cancer allografts in immunocompetent mice to produce spontaneous metastases and describe a pilot study using this method of tumor resection. Intact male FVB/NCrl mice (n = 9) were inoculated subcutaneously with Myc-CaP cells. Tumors were surgically resected, and mice were monitored for tumor recurrence. Animals were euthanized or died, and a full set of tissues was collected for histopathologic examination. Tumors took an average of 44 days (range 23–61) to reach 1.7 cm in any direction. All tumors were resectable, and resection of the tumors increased the study length by 70 days (range 30–121). One mouse was euthanized early of an unrelated cause, and of eight remaining mice, four developed tumor recurrence at the site of resection. One mouse developed bone metastases, one mouse developed metastases to the abdominal cavity, and two mice showed signs of local invasion. This study demonstrates that resection of subcutaneous Myc-CaP cell allografts in mice results in local tumor recurrence and the development of distant metastases, providing a new model system to study prostate cancer metastasis in vivo.
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spelling pubmed-90931822022-05-12 Modeling Human Prostate Cancer Metastasis in Mice via Resection of Subcutaneous Allografts Peiffer, Lauren B. Hicks, Jessica Sosa, Rebecca Y. De Marzo, Angelo M. Sfanos, Karen S. Maynard, Janielle P. Front Oncol Oncology The 5-year survival rate for patients diagnosed with distant metastatic prostate cancer in the United States is 30.6%. Therefore, there is a great need to develop in vivo model systems to study prostate cancer metastasis and to test potential therapeutics. Most murine prostate cancer metastatic models involve intracardiac or intraosseous implantation of cancer cells, which bypass the early stages of tumor cell migration and invasion. Herein we provide a detailed protocol for a novel method of resecting subcutaneous prostate cancer allografts in immunocompetent mice to produce spontaneous metastases and describe a pilot study using this method of tumor resection. Intact male FVB/NCrl mice (n = 9) were inoculated subcutaneously with Myc-CaP cells. Tumors were surgically resected, and mice were monitored for tumor recurrence. Animals were euthanized or died, and a full set of tissues was collected for histopathologic examination. Tumors took an average of 44 days (range 23–61) to reach 1.7 cm in any direction. All tumors were resectable, and resection of the tumors increased the study length by 70 days (range 30–121). One mouse was euthanized early of an unrelated cause, and of eight remaining mice, four developed tumor recurrence at the site of resection. One mouse developed bone metastases, one mouse developed metastases to the abdominal cavity, and two mice showed signs of local invasion. This study demonstrates that resection of subcutaneous Myc-CaP cell allografts in mice results in local tumor recurrence and the development of distant metastases, providing a new model system to study prostate cancer metastasis in vivo. Frontiers Media S.A. 2022-04-27 /pmc/articles/PMC9093182/ /pubmed/35574356 http://dx.doi.org/10.3389/fonc.2022.877536 Text en Copyright © 2022 Peiffer, Hicks, Sosa, De Marzo, Sfanos and Maynard https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Peiffer, Lauren B.
Hicks, Jessica
Sosa, Rebecca Y.
De Marzo, Angelo M.
Sfanos, Karen S.
Maynard, Janielle P.
Modeling Human Prostate Cancer Metastasis in Mice via Resection of Subcutaneous Allografts
title Modeling Human Prostate Cancer Metastasis in Mice via Resection of Subcutaneous Allografts
title_full Modeling Human Prostate Cancer Metastasis in Mice via Resection of Subcutaneous Allografts
title_fullStr Modeling Human Prostate Cancer Metastasis in Mice via Resection of Subcutaneous Allografts
title_full_unstemmed Modeling Human Prostate Cancer Metastasis in Mice via Resection of Subcutaneous Allografts
title_short Modeling Human Prostate Cancer Metastasis in Mice via Resection of Subcutaneous Allografts
title_sort modeling human prostate cancer metastasis in mice via resection of subcutaneous allografts
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9093182/
https://www.ncbi.nlm.nih.gov/pubmed/35574356
http://dx.doi.org/10.3389/fonc.2022.877536
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