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Melatonin Rescues Photosynthesis and Triggers Antioxidant Defense Response in Cucumis sativus Plants Challenged by Low Temperature and High Humidity

Environmental factors such as low temperature (LT) and high humidity (HH) hinder plant growth and development in plastic tunnels and solar greenhouses in the cold season. In this study, we examined the effect of melatonin (MT) on shoot-based tolerance to LT and HH conditions in cucumber (Cucumis sat...

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Autores principales: Amin, Bakht, Atif, Muhammad Jawaad, Meng, Huanwen, Ali, Muhammad, Li, Shuju, Alharby, Hesham F., Majrashi, Ali, Hakeem, Khalid Rehman, Cheng, Zhihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094117/
https://www.ncbi.nlm.nih.gov/pubmed/35574101
http://dx.doi.org/10.3389/fpls.2022.855900
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author Amin, Bakht
Atif, Muhammad Jawaad
Meng, Huanwen
Ali, Muhammad
Li, Shuju
Alharby, Hesham F.
Majrashi, Ali
Hakeem, Khalid Rehman
Cheng, Zhihui
author_facet Amin, Bakht
Atif, Muhammad Jawaad
Meng, Huanwen
Ali, Muhammad
Li, Shuju
Alharby, Hesham F.
Majrashi, Ali
Hakeem, Khalid Rehman
Cheng, Zhihui
author_sort Amin, Bakht
collection PubMed
description Environmental factors such as low temperature (LT) and high humidity (HH) hinder plant growth and development in plastic tunnels and solar greenhouses in the cold season. In this study, we examined the effect of melatonin (MT) on shoot-based tolerance to LT and HH conditions in cucumber (Cucumis sativus) seedlings and explored its underlying mechanism. LT and HH stress inhibited growth and biomass accumulation, produced leaf chlorosis, led to oxidative stress, lowered chlorophyll and carotenoid contents, reduced photosynthetic and photosystem II (PSII) activities, and increased the level of intercellular carbon dioxide and the non-photochemical quenching of photosystem I (PSI) and PSII. However, foliar application of MT significantly improved the morphological indices and photosynthetic efficiency of cucumber seedlings, which entailed the elevation of electrolyte leakage, lipid peroxidation, and reactive oxygen species accumulation by boosting the antioxidant enzyme defense system under LT and HH conditions. Additionally, the measurement of nitrogen (N), magnesium (Mg), and iron (Fe) contents in roots and leaves showed that MT significantly augmented the nutrient uptake of cucumber seedlings exposed to LT and HH stresses. Furthermore, MT application increased the transcripts levels of genes encoding antioxidant enzymes under LT and HH conditions, whereas treatment with LT and HH suppressed these genes, suggesting that MT application increases the LT and HH tolerance of cucumber seedlings. Overall, our results suggest that MT application increases the tolerance of cucumber seedlings to LT and HH stress by enhancing the plant morphometric parameters, regulating PSI and PSII, and activating the antioxidant defense mechanism. Thus, the exogenous application of MT could be potentially employed as a strategy to improve the LT and HH tolerance of cucumber.
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spelling pubmed-90941172022-05-12 Melatonin Rescues Photosynthesis and Triggers Antioxidant Defense Response in Cucumis sativus Plants Challenged by Low Temperature and High Humidity Amin, Bakht Atif, Muhammad Jawaad Meng, Huanwen Ali, Muhammad Li, Shuju Alharby, Hesham F. Majrashi, Ali Hakeem, Khalid Rehman Cheng, Zhihui Front Plant Sci Plant Science Environmental factors such as low temperature (LT) and high humidity (HH) hinder plant growth and development in plastic tunnels and solar greenhouses in the cold season. In this study, we examined the effect of melatonin (MT) on shoot-based tolerance to LT and HH conditions in cucumber (Cucumis sativus) seedlings and explored its underlying mechanism. LT and HH stress inhibited growth and biomass accumulation, produced leaf chlorosis, led to oxidative stress, lowered chlorophyll and carotenoid contents, reduced photosynthetic and photosystem II (PSII) activities, and increased the level of intercellular carbon dioxide and the non-photochemical quenching of photosystem I (PSI) and PSII. However, foliar application of MT significantly improved the morphological indices and photosynthetic efficiency of cucumber seedlings, which entailed the elevation of electrolyte leakage, lipid peroxidation, and reactive oxygen species accumulation by boosting the antioxidant enzyme defense system under LT and HH conditions. Additionally, the measurement of nitrogen (N), magnesium (Mg), and iron (Fe) contents in roots and leaves showed that MT significantly augmented the nutrient uptake of cucumber seedlings exposed to LT and HH stresses. Furthermore, MT application increased the transcripts levels of genes encoding antioxidant enzymes under LT and HH conditions, whereas treatment with LT and HH suppressed these genes, suggesting that MT application increases the LT and HH tolerance of cucumber seedlings. Overall, our results suggest that MT application increases the tolerance of cucumber seedlings to LT and HH stress by enhancing the plant morphometric parameters, regulating PSI and PSII, and activating the antioxidant defense mechanism. Thus, the exogenous application of MT could be potentially employed as a strategy to improve the LT and HH tolerance of cucumber. Frontiers Media S.A. 2022-04-27 /pmc/articles/PMC9094117/ /pubmed/35574101 http://dx.doi.org/10.3389/fpls.2022.855900 Text en Copyright © 2022 Amin, Atif, Meng, Ali, Li, Alharby, Majrashi, Hakeem and Cheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Amin, Bakht
Atif, Muhammad Jawaad
Meng, Huanwen
Ali, Muhammad
Li, Shuju
Alharby, Hesham F.
Majrashi, Ali
Hakeem, Khalid Rehman
Cheng, Zhihui
Melatonin Rescues Photosynthesis and Triggers Antioxidant Defense Response in Cucumis sativus Plants Challenged by Low Temperature and High Humidity
title Melatonin Rescues Photosynthesis and Triggers Antioxidant Defense Response in Cucumis sativus Plants Challenged by Low Temperature and High Humidity
title_full Melatonin Rescues Photosynthesis and Triggers Antioxidant Defense Response in Cucumis sativus Plants Challenged by Low Temperature and High Humidity
title_fullStr Melatonin Rescues Photosynthesis and Triggers Antioxidant Defense Response in Cucumis sativus Plants Challenged by Low Temperature and High Humidity
title_full_unstemmed Melatonin Rescues Photosynthesis and Triggers Antioxidant Defense Response in Cucumis sativus Plants Challenged by Low Temperature and High Humidity
title_short Melatonin Rescues Photosynthesis and Triggers Antioxidant Defense Response in Cucumis sativus Plants Challenged by Low Temperature and High Humidity
title_sort melatonin rescues photosynthesis and triggers antioxidant defense response in cucumis sativus plants challenged by low temperature and high humidity
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094117/
https://www.ncbi.nlm.nih.gov/pubmed/35574101
http://dx.doi.org/10.3389/fpls.2022.855900
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