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Effect of lncRNA MALAT1 on the Granulosa Cell Proliferation and Pregnancy Outcome in Patients With PCOS

Follicle arrest is one of the main characteristics of polycystic ovary syndrome (PCOS), the most common endocrinological disorder in reproductive-aged women. Increasing evidence proves that high anti-Mullerian hormone (AMH) levels may play an important role in follicular development. Long noncoding...

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Autores principales: Tu, Mixue, Wu, Yiqing, Wang, Feixia, Huang, Yun, Qian, Yuli, Li, Jingyi, Lv, Pingping, Ying, Yanyun, Liu, Juan, Liu, Yifeng, Zhang, Runju, Zhao, Wei, Zhang, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094420/
https://www.ncbi.nlm.nih.gov/pubmed/35573984
http://dx.doi.org/10.3389/fendo.2022.825431
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author Tu, Mixue
Wu, Yiqing
Wang, Feixia
Huang, Yun
Qian, Yuli
Li, Jingyi
Lv, Pingping
Ying, Yanyun
Liu, Juan
Liu, Yifeng
Zhang, Runju
Zhao, Wei
Zhang, Dan
author_facet Tu, Mixue
Wu, Yiqing
Wang, Feixia
Huang, Yun
Qian, Yuli
Li, Jingyi
Lv, Pingping
Ying, Yanyun
Liu, Juan
Liu, Yifeng
Zhang, Runju
Zhao, Wei
Zhang, Dan
author_sort Tu, Mixue
collection PubMed
description Follicle arrest is one of the main characteristics of polycystic ovary syndrome (PCOS), the most common endocrinological disorder in reproductive-aged women. Increasing evidence proves that high anti-Mullerian hormone (AMH) levels may play an important role in follicular development. Long noncoding RNA (lncRNA) with a length of more than 200 nt is widely involved in the directional differentiation, growth, and development of cells, whereas whether lncRNA is involved in AMH’s role in follicular development is unknown. In this study, we analyzed lncRNA expression in ovarian granulosa cells (GCs) collected from women with and without PCOS via high-throughput sequencing. The results showed that a total of 79 noncoding transcripts were differently expressed in GCs of PCOS patients, including upregulated lncRNA MALAT1. The upregulation of MALAT1 was further confirmed by RT-qPCR in GCs from a larger cohort of PCOS patients. Furthermore, knockdown MALAT1 can promote the proliferation of KGN cell in vitro. These data suggested a role for MALAT1 in the development of PCOS. Meanwhile, MALAT1 and phosphorylated SMAD 1/5 (Ser463/465) protein were upregulated in KGN cells after exogenous AMH stimulation, which identified AMH perhaps as a regulator for the expression of MALAT1. We also found that MALAT1 can predict clinical pregnancy outcome to a certain extent by ROC curve analysis (area: 0.771, p = 0.007, 95% CI: 0.617–0.925, sensitivity: 57.1%, specificity: 91.7%). Thus, our findings revealed a role of lncRNA MALAT1 in inhibiting granulosa cell proliferation and may be correlated with pregnancy outcome in PCOS.
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spelling pubmed-90944202022-05-12 Effect of lncRNA MALAT1 on the Granulosa Cell Proliferation and Pregnancy Outcome in Patients With PCOS Tu, Mixue Wu, Yiqing Wang, Feixia Huang, Yun Qian, Yuli Li, Jingyi Lv, Pingping Ying, Yanyun Liu, Juan Liu, Yifeng Zhang, Runju Zhao, Wei Zhang, Dan Front Endocrinol (Lausanne) Endocrinology Follicle arrest is one of the main characteristics of polycystic ovary syndrome (PCOS), the most common endocrinological disorder in reproductive-aged women. Increasing evidence proves that high anti-Mullerian hormone (AMH) levels may play an important role in follicular development. Long noncoding RNA (lncRNA) with a length of more than 200 nt is widely involved in the directional differentiation, growth, and development of cells, whereas whether lncRNA is involved in AMH’s role in follicular development is unknown. In this study, we analyzed lncRNA expression in ovarian granulosa cells (GCs) collected from women with and without PCOS via high-throughput sequencing. The results showed that a total of 79 noncoding transcripts were differently expressed in GCs of PCOS patients, including upregulated lncRNA MALAT1. The upregulation of MALAT1 was further confirmed by RT-qPCR in GCs from a larger cohort of PCOS patients. Furthermore, knockdown MALAT1 can promote the proliferation of KGN cell in vitro. These data suggested a role for MALAT1 in the development of PCOS. Meanwhile, MALAT1 and phosphorylated SMAD 1/5 (Ser463/465) protein were upregulated in KGN cells after exogenous AMH stimulation, which identified AMH perhaps as a regulator for the expression of MALAT1. We also found that MALAT1 can predict clinical pregnancy outcome to a certain extent by ROC curve analysis (area: 0.771, p = 0.007, 95% CI: 0.617–0.925, sensitivity: 57.1%, specificity: 91.7%). Thus, our findings revealed a role of lncRNA MALAT1 in inhibiting granulosa cell proliferation and may be correlated with pregnancy outcome in PCOS. Frontiers Media S.A. 2022-04-27 /pmc/articles/PMC9094420/ /pubmed/35573984 http://dx.doi.org/10.3389/fendo.2022.825431 Text en Copyright © 2022 Tu, Wu, Wang, Huang, Qian, Li, Lv, Ying, Liu, Liu, Zhang, Zhao and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Tu, Mixue
Wu, Yiqing
Wang, Feixia
Huang, Yun
Qian, Yuli
Li, Jingyi
Lv, Pingping
Ying, Yanyun
Liu, Juan
Liu, Yifeng
Zhang, Runju
Zhao, Wei
Zhang, Dan
Effect of lncRNA MALAT1 on the Granulosa Cell Proliferation and Pregnancy Outcome in Patients With PCOS
title Effect of lncRNA MALAT1 on the Granulosa Cell Proliferation and Pregnancy Outcome in Patients With PCOS
title_full Effect of lncRNA MALAT1 on the Granulosa Cell Proliferation and Pregnancy Outcome in Patients With PCOS
title_fullStr Effect of lncRNA MALAT1 on the Granulosa Cell Proliferation and Pregnancy Outcome in Patients With PCOS
title_full_unstemmed Effect of lncRNA MALAT1 on the Granulosa Cell Proliferation and Pregnancy Outcome in Patients With PCOS
title_short Effect of lncRNA MALAT1 on the Granulosa Cell Proliferation and Pregnancy Outcome in Patients With PCOS
title_sort effect of lncrna malat1 on the granulosa cell proliferation and pregnancy outcome in patients with pcos
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094420/
https://www.ncbi.nlm.nih.gov/pubmed/35573984
http://dx.doi.org/10.3389/fendo.2022.825431
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