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Interactive analysis of biosurfactants in fruit-waste fermentation samples using BioSurfDB and MEGAN
Agroindustrial waste, such as fruit residues, are a renewable, abundant, low-cost, commonly-used carbon source. Biosurfactants are molecules of increasing interest due to their multifunctional properties, biodegradable nature and low toxicity, in comparison to synthetic surfactants. A better underst...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095615/ https://www.ncbi.nlm.nih.gov/pubmed/35546170 http://dx.doi.org/10.1038/s41598-022-11753-0 |
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author | Silva, Gabriela Fiori da Gautam, Anupam Duarte, Iolanda Cristina Silveira Delforno, Tiago Palladino Oliveira, Valéria Maia de Huson, Daniel H. |
author_facet | Silva, Gabriela Fiori da Gautam, Anupam Duarte, Iolanda Cristina Silveira Delforno, Tiago Palladino Oliveira, Valéria Maia de Huson, Daniel H. |
author_sort | Silva, Gabriela Fiori da |
collection | PubMed |
description | Agroindustrial waste, such as fruit residues, are a renewable, abundant, low-cost, commonly-used carbon source. Biosurfactants are molecules of increasing interest due to their multifunctional properties, biodegradable nature and low toxicity, in comparison to synthetic surfactants. A better understanding of the associated microbial communities will aid prospecting for biosurfactant-producing microorganisms. In this study, six samples of fruit waste, from oranges, mangoes and mixed fruits, were subjected to autochthonous fermentation, so as to promote the growth of their associated microbiota, followed by short-read metagenomic sequencing. Using the DIAMOND+MEGAN analysis pipeline, taxonomic analysis shows that all six samples are dominated by Proteobacteria, in particular, a common core consisting of the genera Klebsiella, Enterobacter, Stenotrophomonas, Acinetobacter and Escherichia. Functional analysis indicates high similarity among samples and a significant number of reads map to genes that are involved in the biosynthesis of lipopeptide-class biosurfactants. Gene-centric analysis reveals Klebsiella as the main assignment for genes related to putisolvins biosynthesis. To simplify the interactive visualization and exploration of the surfactant-related genes in such samples, we have integrated the BiosurfDB classification into MEGAN and make this available. These results indicate that microbiota obtained from autochthonous fermentation have the genetic potential for biosynthesis of biosurfactants, suggesting that fruit wastes may provide a source of biosurfactant-producing microorganisms, with applications in the agricultural, chemical, food and pharmaceutical industries. |
format | Online Article Text |
id | pubmed-9095615 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90956152022-05-13 Interactive analysis of biosurfactants in fruit-waste fermentation samples using BioSurfDB and MEGAN Silva, Gabriela Fiori da Gautam, Anupam Duarte, Iolanda Cristina Silveira Delforno, Tiago Palladino Oliveira, Valéria Maia de Huson, Daniel H. Sci Rep Article Agroindustrial waste, such as fruit residues, are a renewable, abundant, low-cost, commonly-used carbon source. Biosurfactants are molecules of increasing interest due to their multifunctional properties, biodegradable nature and low toxicity, in comparison to synthetic surfactants. A better understanding of the associated microbial communities will aid prospecting for biosurfactant-producing microorganisms. In this study, six samples of fruit waste, from oranges, mangoes and mixed fruits, were subjected to autochthonous fermentation, so as to promote the growth of their associated microbiota, followed by short-read metagenomic sequencing. Using the DIAMOND+MEGAN analysis pipeline, taxonomic analysis shows that all six samples are dominated by Proteobacteria, in particular, a common core consisting of the genera Klebsiella, Enterobacter, Stenotrophomonas, Acinetobacter and Escherichia. Functional analysis indicates high similarity among samples and a significant number of reads map to genes that are involved in the biosynthesis of lipopeptide-class biosurfactants. Gene-centric analysis reveals Klebsiella as the main assignment for genes related to putisolvins biosynthesis. To simplify the interactive visualization and exploration of the surfactant-related genes in such samples, we have integrated the BiosurfDB classification into MEGAN and make this available. These results indicate that microbiota obtained from autochthonous fermentation have the genetic potential for biosynthesis of biosurfactants, suggesting that fruit wastes may provide a source of biosurfactant-producing microorganisms, with applications in the agricultural, chemical, food and pharmaceutical industries. Nature Publishing Group UK 2022-05-11 /pmc/articles/PMC9095615/ /pubmed/35546170 http://dx.doi.org/10.1038/s41598-022-11753-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Silva, Gabriela Fiori da Gautam, Anupam Duarte, Iolanda Cristina Silveira Delforno, Tiago Palladino Oliveira, Valéria Maia de Huson, Daniel H. Interactive analysis of biosurfactants in fruit-waste fermentation samples using BioSurfDB and MEGAN |
title | Interactive analysis of biosurfactants in fruit-waste fermentation samples using BioSurfDB and MEGAN |
title_full | Interactive analysis of biosurfactants in fruit-waste fermentation samples using BioSurfDB and MEGAN |
title_fullStr | Interactive analysis of biosurfactants in fruit-waste fermentation samples using BioSurfDB and MEGAN |
title_full_unstemmed | Interactive analysis of biosurfactants in fruit-waste fermentation samples using BioSurfDB and MEGAN |
title_short | Interactive analysis of biosurfactants in fruit-waste fermentation samples using BioSurfDB and MEGAN |
title_sort | interactive analysis of biosurfactants in fruit-waste fermentation samples using biosurfdb and megan |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095615/ https://www.ncbi.nlm.nih.gov/pubmed/35546170 http://dx.doi.org/10.1038/s41598-022-11753-0 |
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