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Unlocking the functional potential of polyploid yeasts
Breeding and domestication have generated widely exploited crops, animals and microbes. However, many Saccharomyces cerevisiae industrial strains have complex polyploid genomes and are sterile, preventing genetic improvement strategies based on breeding. Here, we present a strain improvement approac...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095626/ https://www.ncbi.nlm.nih.gov/pubmed/35545616 http://dx.doi.org/10.1038/s41467-022-30221-x |
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author | Mozzachiodi, Simone Krogerus, Kristoffer Gibson, Brian Nicolas, Alain Liti, Gianni |
author_facet | Mozzachiodi, Simone Krogerus, Kristoffer Gibson, Brian Nicolas, Alain Liti, Gianni |
author_sort | Mozzachiodi, Simone |
collection | PubMed |
description | Breeding and domestication have generated widely exploited crops, animals and microbes. However, many Saccharomyces cerevisiae industrial strains have complex polyploid genomes and are sterile, preventing genetic improvement strategies based on breeding. Here, we present a strain improvement approach based on the budding yeasts’ property to promote genetic recombination when meiosis is interrupted and cells return-to-mitotic-growth (RTG). We demonstrate that two unrelated sterile industrial strains with complex triploid and tetraploid genomes are RTG-competent and develop a visual screening for easy and high-throughput identification of recombined RTG clones based on colony phenotypes. Sequencing of the evolved clones reveal unprecedented levels of RTG-induced genome-wide recombination. We generate and extensively phenotype a RTG library and identify clones with superior biotechnological traits. Thus, we propose the RTG-framework as a fully non-GMO workflow to rapidly improve industrial yeasts that can be easily brought to the market. |
format | Online Article Text |
id | pubmed-9095626 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90956262022-05-13 Unlocking the functional potential of polyploid yeasts Mozzachiodi, Simone Krogerus, Kristoffer Gibson, Brian Nicolas, Alain Liti, Gianni Nat Commun Article Breeding and domestication have generated widely exploited crops, animals and microbes. However, many Saccharomyces cerevisiae industrial strains have complex polyploid genomes and are sterile, preventing genetic improvement strategies based on breeding. Here, we present a strain improvement approach based on the budding yeasts’ property to promote genetic recombination when meiosis is interrupted and cells return-to-mitotic-growth (RTG). We demonstrate that two unrelated sterile industrial strains with complex triploid and tetraploid genomes are RTG-competent and develop a visual screening for easy and high-throughput identification of recombined RTG clones based on colony phenotypes. Sequencing of the evolved clones reveal unprecedented levels of RTG-induced genome-wide recombination. We generate and extensively phenotype a RTG library and identify clones with superior biotechnological traits. Thus, we propose the RTG-framework as a fully non-GMO workflow to rapidly improve industrial yeasts that can be easily brought to the market. Nature Publishing Group UK 2022-05-11 /pmc/articles/PMC9095626/ /pubmed/35545616 http://dx.doi.org/10.1038/s41467-022-30221-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Mozzachiodi, Simone Krogerus, Kristoffer Gibson, Brian Nicolas, Alain Liti, Gianni Unlocking the functional potential of polyploid yeasts |
title | Unlocking the functional potential of polyploid yeasts |
title_full | Unlocking the functional potential of polyploid yeasts |
title_fullStr | Unlocking the functional potential of polyploid yeasts |
title_full_unstemmed | Unlocking the functional potential of polyploid yeasts |
title_short | Unlocking the functional potential of polyploid yeasts |
title_sort | unlocking the functional potential of polyploid yeasts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095626/ https://www.ncbi.nlm.nih.gov/pubmed/35545616 http://dx.doi.org/10.1038/s41467-022-30221-x |
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