Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations

Hypertrophic cardiomyopathy (HCM) is a common inherited heart disease with a prevalence of about 0.2%. HCM is typically caused by mutations in genes encoding sarcomere or sarcomere-associated proteins. Here, we characterized induced pluripotent stem cell (iPSC) lines generated from the peripheral bl...

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Autores principales: Zhao, Shane Rui, Shen, Mengcheng, Lee, Chelsea, Zha, Yanjun, Guevara, Julio V., Wheeler, Matthew T., Wu, Joseph C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095754/
https://www.ncbi.nlm.nih.gov/pubmed/34798544
http://dx.doi.org/10.1016/j.scr.2021.102597
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author Zhao, Shane Rui
Shen, Mengcheng
Lee, Chelsea
Zha, Yanjun
Guevara, Julio V.
Wheeler, Matthew T.
Wu, Joseph C.
author_facet Zhao, Shane Rui
Shen, Mengcheng
Lee, Chelsea
Zha, Yanjun
Guevara, Julio V.
Wheeler, Matthew T.
Wu, Joseph C.
author_sort Zhao, Shane Rui
collection PubMed
description Hypertrophic cardiomyopathy (HCM) is a common inherited heart disease with a prevalence of about 0.2%. HCM is typically caused by mutations in genes encoding sarcomere or sarcomere-associated proteins. Here, we characterized induced pluripotent stem cell (iPSC) lines generated from the peripheral blood mononuclear cells of three HCM patients each carrying c.433C > T, c.610C > T, or c.235C > T mutation in the TNNI3 gene by non-integrated Sendai virus. All of the three lines exhibited normal morphology, expression of pluripotent markers, stable karyotype, and the potential of trilineage differentiation. The cardiomyocytes differentiated from these iPSC lines can serve as useful tools to model HCM in vitro.
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spelling pubmed-90957542023-05-12 Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations Zhao, Shane Rui Shen, Mengcheng Lee, Chelsea Zha, Yanjun Guevara, Julio V. Wheeler, Matthew T. Wu, Joseph C. Stem Cell Res Article Hypertrophic cardiomyopathy (HCM) is a common inherited heart disease with a prevalence of about 0.2%. HCM is typically caused by mutations in genes encoding sarcomere or sarcomere-associated proteins. Here, we characterized induced pluripotent stem cell (iPSC) lines generated from the peripheral blood mononuclear cells of three HCM patients each carrying c.433C > T, c.610C > T, or c.235C > T mutation in the TNNI3 gene by non-integrated Sendai virus. All of the three lines exhibited normal morphology, expression of pluripotent markers, stable karyotype, and the potential of trilineage differentiation. The cardiomyocytes differentiated from these iPSC lines can serve as useful tools to model HCM in vitro. 2021-11-12 /pmc/articles/PMC9095754/ /pubmed/34798544 http://dx.doi.org/10.1016/j.scr.2021.102597 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Zhao, Shane Rui
Shen, Mengcheng
Lee, Chelsea
Zha, Yanjun
Guevara, Julio V.
Wheeler, Matthew T.
Wu, Joseph C.
Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations
title Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations
title_full Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations
title_fullStr Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations
title_full_unstemmed Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations
title_short Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations
title_sort generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying tnni3 mutations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095754/
https://www.ncbi.nlm.nih.gov/pubmed/34798544
http://dx.doi.org/10.1016/j.scr.2021.102597
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