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Efficient Green Light Acclimation of the Green Algae Picochlorum sp. Triggering Geranylgeranylated Chlorophylls

In analogy to higher plants, eukaryotic microalgae are thought to be incapable of utilizing green light for growth, due to the “green gap” in the absorbance profiles of their photosynthetic pigments. This study demonstrates, that the marine chlorophyte Picochlorum sp. is able to grow efficiently und...

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Autores principales: Paper, Michael, Glemser, Matthias, Haack, Martina, Lorenzen, Jan, Mehlmer, Norbert, Fuchs, Tobias, Schenk, Gerhard, Garbe, Daniel, Weuster-Botz, Dirk, Eisenreich, Wolfgang, Lakatos, Michael, Brück, Thomas B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095919/
https://www.ncbi.nlm.nih.gov/pubmed/35573232
http://dx.doi.org/10.3389/fbioe.2022.885977
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author Paper, Michael
Glemser, Matthias
Haack, Martina
Lorenzen, Jan
Mehlmer, Norbert
Fuchs, Tobias
Schenk, Gerhard
Garbe, Daniel
Weuster-Botz, Dirk
Eisenreich, Wolfgang
Lakatos, Michael
Brück, Thomas B.
author_facet Paper, Michael
Glemser, Matthias
Haack, Martina
Lorenzen, Jan
Mehlmer, Norbert
Fuchs, Tobias
Schenk, Gerhard
Garbe, Daniel
Weuster-Botz, Dirk
Eisenreich, Wolfgang
Lakatos, Michael
Brück, Thomas B.
author_sort Paper, Michael
collection PubMed
description In analogy to higher plants, eukaryotic microalgae are thought to be incapable of utilizing green light for growth, due to the “green gap” in the absorbance profiles of their photosynthetic pigments. This study demonstrates, that the marine chlorophyte Picochlorum sp. is able to grow efficiently under green light emitting diode (LED) illumination. Picochlorum sp. growth and pigment profiles under blue, red, green and white LED illumination (light intensity: 50–200 μmol m(−2) s(−1)) in bottom-lightened shake flask cultures were evaluated. Green light-treated cultures showed a prolonged initial growth lag phase of one to 2 days, which was subsequently compensated to obtain comparable biomass yields to red and white light controls (approx. 0.8 g(DW) L(−1)). Interestingly, growth and final biomass yields of the green light-treated sample were higher than under blue light with equivalent illumination energies. Further, pigment analysis indicated, that during green light illumination, Picochlorum sp. formed unknown pigments (X1-X4). Pigment concentrations increased with illumination intensity and were most abundant during the exponential growth phase. Mass spectrometry and nuclear magnetic resonance data indicated, that pigments X1-X2 and X3-X4 are derivatives of chlorophyll b and a, which harbor C=C bonds in the phytol side chain similar to geranylgeranylated chlorophylls. Thus, for the first time, the natural accumulation of large pools (approx. 12 mg g(DW) (−1)) of chlorophyll intermediates with incomplete hydrogenation of their phytyl chains is demonstrated for algae under monochromatic green light (Peak λ 510 nm, full width at half maximum 91 nm). The ability to utilize green light offers competitive advantages for enhancing biomass production, particularly under conditions of dense cultures, long light pathways and high light intensity. Green light acclimation for an eukaryotic microalgae in conjunction with the formation of new aberrant geranylgeranylated chlorophylls and high efficiency of growth rates are novel for eukaryotic microalgae. Illumination with green light could enhance productivity in industrial processes and trigger the formation of new metabolites–thus, underlying mechanisms require further investigation.
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spelling pubmed-90959192022-05-13 Efficient Green Light Acclimation of the Green Algae Picochlorum sp. Triggering Geranylgeranylated Chlorophylls Paper, Michael Glemser, Matthias Haack, Martina Lorenzen, Jan Mehlmer, Norbert Fuchs, Tobias Schenk, Gerhard Garbe, Daniel Weuster-Botz, Dirk Eisenreich, Wolfgang Lakatos, Michael Brück, Thomas B. Front Bioeng Biotechnol Bioengineering and Biotechnology In analogy to higher plants, eukaryotic microalgae are thought to be incapable of utilizing green light for growth, due to the “green gap” in the absorbance profiles of their photosynthetic pigments. This study demonstrates, that the marine chlorophyte Picochlorum sp. is able to grow efficiently under green light emitting diode (LED) illumination. Picochlorum sp. growth and pigment profiles under blue, red, green and white LED illumination (light intensity: 50–200 μmol m(−2) s(−1)) in bottom-lightened shake flask cultures were evaluated. Green light-treated cultures showed a prolonged initial growth lag phase of one to 2 days, which was subsequently compensated to obtain comparable biomass yields to red and white light controls (approx. 0.8 g(DW) L(−1)). Interestingly, growth and final biomass yields of the green light-treated sample were higher than under blue light with equivalent illumination energies. Further, pigment analysis indicated, that during green light illumination, Picochlorum sp. formed unknown pigments (X1-X4). Pigment concentrations increased with illumination intensity and were most abundant during the exponential growth phase. Mass spectrometry and nuclear magnetic resonance data indicated, that pigments X1-X2 and X3-X4 are derivatives of chlorophyll b and a, which harbor C=C bonds in the phytol side chain similar to geranylgeranylated chlorophylls. Thus, for the first time, the natural accumulation of large pools (approx. 12 mg g(DW) (−1)) of chlorophyll intermediates with incomplete hydrogenation of their phytyl chains is demonstrated for algae under monochromatic green light (Peak λ 510 nm, full width at half maximum 91 nm). The ability to utilize green light offers competitive advantages for enhancing biomass production, particularly under conditions of dense cultures, long light pathways and high light intensity. Green light acclimation for an eukaryotic microalgae in conjunction with the formation of new aberrant geranylgeranylated chlorophylls and high efficiency of growth rates are novel for eukaryotic microalgae. Illumination with green light could enhance productivity in industrial processes and trigger the formation of new metabolites–thus, underlying mechanisms require further investigation. Frontiers Media S.A. 2022-04-28 /pmc/articles/PMC9095919/ /pubmed/35573232 http://dx.doi.org/10.3389/fbioe.2022.885977 Text en Copyright © 2022 Paper, Glemser, Haack, Lorenzen, Mehlmer, Fuchs, Schenk, Garbe, Weuster-Botz, Eisenreich, Lakatos and Brück. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Paper, Michael
Glemser, Matthias
Haack, Martina
Lorenzen, Jan
Mehlmer, Norbert
Fuchs, Tobias
Schenk, Gerhard
Garbe, Daniel
Weuster-Botz, Dirk
Eisenreich, Wolfgang
Lakatos, Michael
Brück, Thomas B.
Efficient Green Light Acclimation of the Green Algae Picochlorum sp. Triggering Geranylgeranylated Chlorophylls
title Efficient Green Light Acclimation of the Green Algae Picochlorum sp. Triggering Geranylgeranylated Chlorophylls
title_full Efficient Green Light Acclimation of the Green Algae Picochlorum sp. Triggering Geranylgeranylated Chlorophylls
title_fullStr Efficient Green Light Acclimation of the Green Algae Picochlorum sp. Triggering Geranylgeranylated Chlorophylls
title_full_unstemmed Efficient Green Light Acclimation of the Green Algae Picochlorum sp. Triggering Geranylgeranylated Chlorophylls
title_short Efficient Green Light Acclimation of the Green Algae Picochlorum sp. Triggering Geranylgeranylated Chlorophylls
title_sort efficient green light acclimation of the green algae picochlorum sp. triggering geranylgeranylated chlorophylls
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095919/
https://www.ncbi.nlm.nih.gov/pubmed/35573232
http://dx.doi.org/10.3389/fbioe.2022.885977
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