ColoriSens: An open-source and low-cost portable color sensor board for microfluidic integration with wireless communication and fluorescence detection

Microfluidic colorimetric biosensors have shown promising potential for detecting metal cations, anions, organic dyes, drugs, pesticides. As for today, most colorimetric sensors are read by a smartphone or professional optical imaging system, and there is still a lack of an affordable and reliable colorimetric detector for the microfluidic chip. Integrating those reading and detection capabilities into a microfluidic system is essential for point-of-care (POC) detection and can enable more complex microfluidic operations, such as lab-on-a-chip experiments or programmable microfluidics. We developed an open-source colorimetric detection sensor board that can be integrated into the existing microfluidic system. This sensor board has a built-in UV source that enables fluorescence detection. With built-in USB and Wi-Fi connectivity and a set of simple APIs, microfluidic systems can communicate directly with this sensor board, even wirelessly. The sensor was designed for low-cost. With a total build cost of less than 12 EUR per unit, it is ideal for low-cost systems and DIY microfluidic users. Along with the sensor board, we also designed a companion microfluidic chip carrier cartridge which can be modified depending on the chip’s dimension. To demonstrate the sensor, we also developed a cross-platform open-source client application to demonstrate the communication APIs and the functionality of the sensor board.