Cargando…

H1N1 Influenza Virus-Infected Nasal Mucosal Epithelial Progenitor Cells Promote Dendritic Cell Recruitment and Maturation

The barrier function of nasal mucosal epithelial cells plays an irreplaceable role in the spread and expansion of viruses in the body. This study found that influenza A virus H1N1 could induce apoptosis of nasal mucosal epithelial progenitor cells, cause an inflammatory response, and trigger the mat...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Fangyuan, Teng, Zhenxiao, Zhou, Xuanchen, Xu, Runtong, Bing, Xin, Shi, Lei, Guo, Na, Wang, Min, Liu, Chengcheng, Xia, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095954/
https://www.ncbi.nlm.nih.gov/pubmed/35572503
http://dx.doi.org/10.3389/fimmu.2022.879575
_version_ 1784705867264819200
author Zhu, Fangyuan
Teng, Zhenxiao
Zhou, Xuanchen
Xu, Runtong
Bing, Xin
Shi, Lei
Guo, Na
Wang, Min
Liu, Chengcheng
Xia, Ming
author_facet Zhu, Fangyuan
Teng, Zhenxiao
Zhou, Xuanchen
Xu, Runtong
Bing, Xin
Shi, Lei
Guo, Na
Wang, Min
Liu, Chengcheng
Xia, Ming
author_sort Zhu, Fangyuan
collection PubMed
description The barrier function of nasal mucosal epithelial cells plays an irreplaceable role in the spread and expansion of viruses in the body. This study found that influenza A virus H1N1 could induce apoptosis of nasal mucosal epithelial progenitor cells, cause an inflammatory response, and trigger the maturation and recruitment of nasal submucosal dendritic cells (DCs), but the mechanism remained unclear. Therefore, we used RNA sequencing and high-resolution untargeted metabolomics to sequence and perform combined bioinformatic analysis of H1N1 virus-infected nasal mucosal epithelial cells from 6 different patients. The abnormal arginine metabolism signaling pathway caused by H1N1 virus infection was screened out, and arginase inhibitors were used to interfere with the abnormal arginine metabolism and the maturation and recruitment of submucosal DCs caused by the H1N1 virus in vitro and in vivo. We conclude that H1N1 influenza virus promotes the recruitment and maturation of submucosal DCs by causing abnormal arginine metabolism in nasal mucosal epithelial cells, thereby triggering respiratory mucosal immunity.
format Online
Article
Text
id pubmed-9095954
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-90959542022-05-13 H1N1 Influenza Virus-Infected Nasal Mucosal Epithelial Progenitor Cells Promote Dendritic Cell Recruitment and Maturation Zhu, Fangyuan Teng, Zhenxiao Zhou, Xuanchen Xu, Runtong Bing, Xin Shi, Lei Guo, Na Wang, Min Liu, Chengcheng Xia, Ming Front Immunol Immunology The barrier function of nasal mucosal epithelial cells plays an irreplaceable role in the spread and expansion of viruses in the body. This study found that influenza A virus H1N1 could induce apoptosis of nasal mucosal epithelial progenitor cells, cause an inflammatory response, and trigger the maturation and recruitment of nasal submucosal dendritic cells (DCs), but the mechanism remained unclear. Therefore, we used RNA sequencing and high-resolution untargeted metabolomics to sequence and perform combined bioinformatic analysis of H1N1 virus-infected nasal mucosal epithelial cells from 6 different patients. The abnormal arginine metabolism signaling pathway caused by H1N1 virus infection was screened out, and arginase inhibitors were used to interfere with the abnormal arginine metabolism and the maturation and recruitment of submucosal DCs caused by the H1N1 virus in vitro and in vivo. We conclude that H1N1 influenza virus promotes the recruitment and maturation of submucosal DCs by causing abnormal arginine metabolism in nasal mucosal epithelial cells, thereby triggering respiratory mucosal immunity. Frontiers Media S.A. 2022-04-28 /pmc/articles/PMC9095954/ /pubmed/35572503 http://dx.doi.org/10.3389/fimmu.2022.879575 Text en Copyright © 2022 Zhu, Teng, Zhou, Xu, Bing, Shi, Guo, Wang, Liu and Xia https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Zhu, Fangyuan
Teng, Zhenxiao
Zhou, Xuanchen
Xu, Runtong
Bing, Xin
Shi, Lei
Guo, Na
Wang, Min
Liu, Chengcheng
Xia, Ming
H1N1 Influenza Virus-Infected Nasal Mucosal Epithelial Progenitor Cells Promote Dendritic Cell Recruitment and Maturation
title H1N1 Influenza Virus-Infected Nasal Mucosal Epithelial Progenitor Cells Promote Dendritic Cell Recruitment and Maturation
title_full H1N1 Influenza Virus-Infected Nasal Mucosal Epithelial Progenitor Cells Promote Dendritic Cell Recruitment and Maturation
title_fullStr H1N1 Influenza Virus-Infected Nasal Mucosal Epithelial Progenitor Cells Promote Dendritic Cell Recruitment and Maturation
title_full_unstemmed H1N1 Influenza Virus-Infected Nasal Mucosal Epithelial Progenitor Cells Promote Dendritic Cell Recruitment and Maturation
title_short H1N1 Influenza Virus-Infected Nasal Mucosal Epithelial Progenitor Cells Promote Dendritic Cell Recruitment and Maturation
title_sort h1n1 influenza virus-infected nasal mucosal epithelial progenitor cells promote dendritic cell recruitment and maturation
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095954/
https://www.ncbi.nlm.nih.gov/pubmed/35572503
http://dx.doi.org/10.3389/fimmu.2022.879575
work_keys_str_mv AT zhufangyuan h1n1influenzavirusinfectednasalmucosalepithelialprogenitorcellspromotedendriticcellrecruitmentandmaturation
AT tengzhenxiao h1n1influenzavirusinfectednasalmucosalepithelialprogenitorcellspromotedendriticcellrecruitmentandmaturation
AT zhouxuanchen h1n1influenzavirusinfectednasalmucosalepithelialprogenitorcellspromotedendriticcellrecruitmentandmaturation
AT xuruntong h1n1influenzavirusinfectednasalmucosalepithelialprogenitorcellspromotedendriticcellrecruitmentandmaturation
AT bingxin h1n1influenzavirusinfectednasalmucosalepithelialprogenitorcellspromotedendriticcellrecruitmentandmaturation
AT shilei h1n1influenzavirusinfectednasalmucosalepithelialprogenitorcellspromotedendriticcellrecruitmentandmaturation
AT guona h1n1influenzavirusinfectednasalmucosalepithelialprogenitorcellspromotedendriticcellrecruitmentandmaturation
AT wangmin h1n1influenzavirusinfectednasalmucosalepithelialprogenitorcellspromotedendriticcellrecruitmentandmaturation
AT liuchengcheng h1n1influenzavirusinfectednasalmucosalepithelialprogenitorcellspromotedendriticcellrecruitmentandmaturation
AT xiaming h1n1influenzavirusinfectednasalmucosalepithelialprogenitorcellspromotedendriticcellrecruitmentandmaturation