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Combined effects of exercise training and D‐allulose intake on endurance capacity in mice

This study investigated the combined effects of exercise training and D‐allulose intake on endurance capacity in mice. Male C57BL/6J mice were fed either a control diet (Con) or a 3% D‐allulose diet (Allu) and further divided into the sedentary (Sed) or exercise training (Ex) groups (Con‐Sed, Con‐Ex...

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Autores principales: Tsuzuki, Takamasa, Suzuki, Ryo, Kajun, Risa, Yamada, Takako, Iida, Tetsuo, Liu, Bingyang, Koike, Teruhiko, Toyoda, Yukiyasu, Negishi, Takayuki, Yukawa, Kazunori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095992/
https://www.ncbi.nlm.nih.gov/pubmed/35546434
http://dx.doi.org/10.14814/phy2.15297
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author Tsuzuki, Takamasa
Suzuki, Ryo
Kajun, Risa
Yamada, Takako
Iida, Tetsuo
Liu, Bingyang
Koike, Teruhiko
Toyoda, Yukiyasu
Negishi, Takayuki
Yukawa, Kazunori
author_facet Tsuzuki, Takamasa
Suzuki, Ryo
Kajun, Risa
Yamada, Takako
Iida, Tetsuo
Liu, Bingyang
Koike, Teruhiko
Toyoda, Yukiyasu
Negishi, Takayuki
Yukawa, Kazunori
author_sort Tsuzuki, Takamasa
collection PubMed
description This study investigated the combined effects of exercise training and D‐allulose intake on endurance capacity in mice. Male C57BL/6J mice were fed either a control diet (Con) or a 3% D‐allulose diet (Allu) and further divided into the sedentary (Sed) or exercise training (Ex) groups (Con‐Sed, Con‐Ex, Allu‐Sed, Allu‐Ex, respectively; n = 6–7/group). The mice in the Ex groups were trained on a motor‐driven treadmill 5 days/week for 4 weeks (15–18 m/min, 60 min). After the exercise training period, all mice underwent an exhaustive running test to assess their endurance capacity. At 48 h after the running test, the mice in the Ex groups were subjected to run at 18 m/min for 60 min again. Then the gastrocnemius muscle and liver were sampled immediately after the exercise bout. The running time until exhaustion tended to be higher in the Allu‐Ex than in the Con‐Ex group (p = 0.08). The muscle glycogen content was significantly lower in the Con‐Ex than in the Con‐Sed group and was significantly higher in the Allu‐Ex than in the Con‐Ex group (p < 0.05). Moreover, exercise training increased the phosphorylation levels of adenosine monophosphate‐activated protein kinase (AMPK) in the muscle and liver. The phosphorylation levels of acetyl coenzyme A carboxylase (ACC), a downstream of AMPK, in the muscle and liver were significantly higher in the Allu‐Ex than in the Con‐Sed group (p < 0.05), suggesting that the combination of exercise training and D‐allulose might have activated the AMPK‐ACC signaling pathway, which is associated with fatty acid oxidation in the muscle and liver. Taken together, our data suggested the combination of exercise training and D‐allulose intake as an effective strategy to upregulate endurance capacity in mice. This may be associated with sparing glycogen content and enhancing activation of AMPK‐ACC signaling in the skeletal muscle.
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spelling pubmed-90959922022-05-18 Combined effects of exercise training and D‐allulose intake on endurance capacity in mice Tsuzuki, Takamasa Suzuki, Ryo Kajun, Risa Yamada, Takako Iida, Tetsuo Liu, Bingyang Koike, Teruhiko Toyoda, Yukiyasu Negishi, Takayuki Yukawa, Kazunori Physiol Rep Original Articles This study investigated the combined effects of exercise training and D‐allulose intake on endurance capacity in mice. Male C57BL/6J mice were fed either a control diet (Con) or a 3% D‐allulose diet (Allu) and further divided into the sedentary (Sed) or exercise training (Ex) groups (Con‐Sed, Con‐Ex, Allu‐Sed, Allu‐Ex, respectively; n = 6–7/group). The mice in the Ex groups were trained on a motor‐driven treadmill 5 days/week for 4 weeks (15–18 m/min, 60 min). After the exercise training period, all mice underwent an exhaustive running test to assess their endurance capacity. At 48 h after the running test, the mice in the Ex groups were subjected to run at 18 m/min for 60 min again. Then the gastrocnemius muscle and liver were sampled immediately after the exercise bout. The running time until exhaustion tended to be higher in the Allu‐Ex than in the Con‐Ex group (p = 0.08). The muscle glycogen content was significantly lower in the Con‐Ex than in the Con‐Sed group and was significantly higher in the Allu‐Ex than in the Con‐Ex group (p < 0.05). Moreover, exercise training increased the phosphorylation levels of adenosine monophosphate‐activated protein kinase (AMPK) in the muscle and liver. The phosphorylation levels of acetyl coenzyme A carboxylase (ACC), a downstream of AMPK, in the muscle and liver were significantly higher in the Allu‐Ex than in the Con‐Sed group (p < 0.05), suggesting that the combination of exercise training and D‐allulose might have activated the AMPK‐ACC signaling pathway, which is associated with fatty acid oxidation in the muscle and liver. Taken together, our data suggested the combination of exercise training and D‐allulose intake as an effective strategy to upregulate endurance capacity in mice. This may be associated with sparing glycogen content and enhancing activation of AMPK‐ACC signaling in the skeletal muscle. John Wiley and Sons Inc. 2022-05-11 /pmc/articles/PMC9095992/ /pubmed/35546434 http://dx.doi.org/10.14814/phy2.15297 Text en © 2022 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Tsuzuki, Takamasa
Suzuki, Ryo
Kajun, Risa
Yamada, Takako
Iida, Tetsuo
Liu, Bingyang
Koike, Teruhiko
Toyoda, Yukiyasu
Negishi, Takayuki
Yukawa, Kazunori
Combined effects of exercise training and D‐allulose intake on endurance capacity in mice
title Combined effects of exercise training and D‐allulose intake on endurance capacity in mice
title_full Combined effects of exercise training and D‐allulose intake on endurance capacity in mice
title_fullStr Combined effects of exercise training and D‐allulose intake on endurance capacity in mice
title_full_unstemmed Combined effects of exercise training and D‐allulose intake on endurance capacity in mice
title_short Combined effects of exercise training and D‐allulose intake on endurance capacity in mice
title_sort combined effects of exercise training and d‐allulose intake on endurance capacity in mice
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095992/
https://www.ncbi.nlm.nih.gov/pubmed/35546434
http://dx.doi.org/10.14814/phy2.15297
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