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Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in calves
Understanding the immune dynamics in the respiratory mucosa of calves is necessary for a good management of bovine respiratory disease. Immune dynamics in the respiratory mucosa in humans and experimental animals has been assessed by flow cytometric analysis of bronchoalveolar lavage fluid (BALF); h...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096045/ https://www.ncbi.nlm.nih.gov/pubmed/35153256 http://dx.doi.org/10.1292/jvms.21-0522 |
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author | ISHIKAWA, Shingo MIYAZAWA, Masataka ZIBIKI, Yoshinori KAMIKAKIMOTO, Rie HOBO, Seiji |
author_facet | ISHIKAWA, Shingo MIYAZAWA, Masataka ZIBIKI, Yoshinori KAMIKAKIMOTO, Rie HOBO, Seiji |
author_sort | ISHIKAWA, Shingo |
collection | PubMed |
description | Understanding the immune dynamics in the respiratory mucosa of calves is necessary for a good management of bovine respiratory disease. Immune dynamics in the respiratory mucosa in humans and experimental animals has been assessed by flow cytometric analysis of bronchoalveolar lavage fluid (BALF); however, few reports have addressed this subject in calves. The aim of this study was to establish a universal method to analyze bronchoalveolar lavage fluid (BALF) by flow cytometry and to obtain basic knowledge of bovine respiratory mucosal immune dynamics. We investigated the immune cell populations in BALF and evaluated the surface antigen expression of alveolar macrophages in calves using flow cytometer. To further analyze the surface antigen variation observed in alveolar macrophages in detail, stimulation assays were performed in vitro. BALF cells were separated into three distinct populations based on their light scatter plot, which were considered to be macrophages, lymphocytes, and neutrophils. In most individuals, most of the BALF immune cells were alveolar macrophages, but an increased proportion of lymphocytes and neutrophils was observed in some individuals. Analysis of each surface antigen expression in alveolar macrophages showed that CD21 and MHC class II expression changed in response to changes in the leukocyte population. Moreover, when alveolar macrophages were stimulated with interferon-γ in vitro, the expression of CD21 was drastically reduced and MHC class II was increased, suggesting that functional changes in alveolar macrophages themselves are involved in the immune dynamics. |
format | Online Article Text |
id | pubmed-9096045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-90960452022-05-18 Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in calves ISHIKAWA, Shingo MIYAZAWA, Masataka ZIBIKI, Yoshinori KAMIKAKIMOTO, Rie HOBO, Seiji J Vet Med Sci Immunology Understanding the immune dynamics in the respiratory mucosa of calves is necessary for a good management of bovine respiratory disease. Immune dynamics in the respiratory mucosa in humans and experimental animals has been assessed by flow cytometric analysis of bronchoalveolar lavage fluid (BALF); however, few reports have addressed this subject in calves. The aim of this study was to establish a universal method to analyze bronchoalveolar lavage fluid (BALF) by flow cytometry and to obtain basic knowledge of bovine respiratory mucosal immune dynamics. We investigated the immune cell populations in BALF and evaluated the surface antigen expression of alveolar macrophages in calves using flow cytometer. To further analyze the surface antigen variation observed in alveolar macrophages in detail, stimulation assays were performed in vitro. BALF cells were separated into three distinct populations based on their light scatter plot, which were considered to be macrophages, lymphocytes, and neutrophils. In most individuals, most of the BALF immune cells were alveolar macrophages, but an increased proportion of lymphocytes and neutrophils was observed in some individuals. Analysis of each surface antigen expression in alveolar macrophages showed that CD21 and MHC class II expression changed in response to changes in the leukocyte population. Moreover, when alveolar macrophages were stimulated with interferon-γ in vitro, the expression of CD21 was drastically reduced and MHC class II was increased, suggesting that functional changes in alveolar macrophages themselves are involved in the immune dynamics. The Japanese Society of Veterinary Science 2022-02-10 2022-04 /pmc/articles/PMC9096045/ /pubmed/35153256 http://dx.doi.org/10.1292/jvms.21-0522 Text en ©2022 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Immunology ISHIKAWA, Shingo MIYAZAWA, Masataka ZIBIKI, Yoshinori KAMIKAKIMOTO, Rie HOBO, Seiji Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in calves |
title | Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in
calves |
title_full | Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in
calves |
title_fullStr | Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in
calves |
title_full_unstemmed | Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in
calves |
title_short | Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in
calves |
title_sort | flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in
calves |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096045/ https://www.ncbi.nlm.nih.gov/pubmed/35153256 http://dx.doi.org/10.1292/jvms.21-0522 |
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